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  • 1
    Electronic Resource
    Electronic Resource
    Regeneration of bovine and octopus opsins in situ with natural and artificial retinals (1989)
    s.l. : American Chemical Society
    Biochemistry 28 (1989), S. 2732-2739 
    Details
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/bi00432a055
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  • 2
    Electronic Resource
    Electronic Resource
    Antimutagenic unusual amino acids from plants (1985)
    Springer
    Cellular and molecular life sciences 41 (1985), S. 1622-1623 
    Details
    ISSN: 1420-9071
    Keywords: Antimutagens ; natural product ; unusual amino acids ; isolation and structural determination ; Liliaceae ; Sapindaceae ; Salmonella typhimurium TA100
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Five unusual amino acids were identified as antimutagens against spontaneous mutation ofSalmonella typhimurium TA100: L-azetidine-2-carboxylic acid (1) from Liliaceae plants, α-(methylenecyclopropyl)glycine (2) fromLitchi chinensis seeds, and 2-amino-4-methylhex-5-ynoic acid (3), hypoglycin A (4), and (2S, 4R)-2-amino-4-hydroxyhept-6-ynoic acid (5) fromEuphoria longana seeds. The absolute stereochemistry of5 was determined by its chiral synthesis from L-allylglycine, proving that5 is the C-4 epimer of the amino acid previously isolated from dried longan seeds.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01964840
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  • 3
    Electronic Resource
    Electronic Resource
    Formation of Oligosaccharides During Hydrolysis of Lactose in Milk Using β-Galactosidase from Bacillus circulans (1985)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 50 (1985), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The characteristics of β-galactosidase from Bacillus circulans, and its suitability for hydrolysis of milk lactose were compared with those of Escherichia coli and Kluyveromyces lactis enzymes. Enzyme activity of β-galactosidase from B. circulans was not affected by major cations and proteins present in skim milk, whereas milk proteins and sodium and calcium ions decreased the activity of the enzymes from E. coli and K. lactis. The enzyme from B. circulans produced less oligosaccharides than E. coli and K. lactis during hydrolysis of lactose in skim milk at different lactose concentrations. Oligosaccharides produced by the enzyme from B. circulans disappeared almost completely at the latter stage of hydrolysis, while a large amount of oligosaccharides were still detected even at 98% conversion of lactose with enzymes from E. coli and K. lactis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.1985.tb10543.x
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  • 4
    Electronic Resource
    Electronic Resource
    Penning ionization ternary gas mixtures for diffuse discharge switching applications (1985)
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 58 (1985), S. 633-641 
    Details
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: The increase in the total ionization produced by high-energy α particles in Ar/C2F6 mixtures, which have conduction and insulation properties appropriate for use in diffuse discharge switching applications by addition of low ionization energy additives has been quantitatively studied. The energy to produce an electron-positive ion pair (ip) W in C2F6 was found to be 34.7 eV/ip; this rather high value is attributed to the large cross section for electron impact-induced dissociation of C2F6. The W values of Ar/C2F6 mixtures have also been measured and are reported; they increase with increasing C2F6 content. The W values of Ar/C2F6 binary gas mixtures have been found to decrease by addition of C2H2 or 2-C4H8. Quantitative measurements of the W values of the ternary gas mixtures are reported. The amounts of C2H2 or 2-C4H8 in Ar/C2F6, which maximize the increase in total ionization have been estimated; some of these ternary gas mixtures may be useful for diffuse discharge switches sustained by external electron beams. The experimental results on the W values for the binary and ternary gas mixtures studied have been modeled and good agreement has been found between the experimental and the calculated results. From an analysis of the data it has been found that the α-particle energy is partitioned between C2F6 and Ar approximately in the ratio 4.2:1. It has also been found that at the argon pressures employed, the probability of deexcitation of excited argon atoms by C2F6 (or C2H2 or 2-C4H8) molecules is more than three orders of magnitude larger than the probability of radiative deexcitation of the excited argon atoms.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.336201
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  • 5
    Electronic Resource
    Electronic Resource
    Different instability of nuclear DNA at acid hydrolysis in cancerous and noncancerous cells as revealed by fluorescent staining with acridine orange (1986)
    Springer
    Histochemistry and cell biology 84 (1986), S. 556-560 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Ehrlich cancer cells and inflammatory cells in mouse ascitic fluid were hydrolyzed and stained with acridine orange (AO). The AO hydrolysis curves for G1/G2+M phase cancer cells and inflammatory cells were differentially determined using flow cytometry by monitoring the metachromatic red-shifted fluorescence of the fluorochrome bound to the single-stranded DNA produced by acid hydrolysis. By computer fitting of the Bateman function to the hydrolysis curves, the kinetic parameters k 1 (rate constant for the degradation of the produced single-stranded DNA), and y 0 (theoretical value of the single-stranded DNA present initially) were determined. It was found that the k 2 value, which reflects the degree of DNA instability, was much higher for cancer cells in both the G1 and G2+M phases than for inflammatory cells. This finding led us to develop a method for the differential AO staining of cancer cells and non-cancerous cells utilizing the different degree of DNA instability at acid hydrolysis. AO staining after hydrolysis with 2N HCl at 30°C for 8.5 min was found to be the optimal method. In the 60 cases of human malignant epithelial and nonepithelial tumors tested, all of the malignant tumor cells emitted metachromatic red fluorescence, while all of the nonmalignant tumor cells (5 cases of benign tumor) and normal cells emitted orthochromatic green fluorescence when observed with a violet excitation light under a fluorescence microscope. This new technique can be a useful tool for the screening of malignancy in exfoliative cytology and also for basic cancer research.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00482991
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  • 6
    Electronic Resource
    Electronic Resource
    Continuous Synthesis of N-(Benzyloxycarbonyl)- L -Aspartyl- L -Phenylalanine Methyl Ester with Immobilized Thermolysin in an Organic Solvent (1985)
    [s.l.] : Nature Publishing Company
    Nature biotechnology 3 (1985), S. 459-464 
    Details
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] N - (benzyloxycarbonyl) - L - aspartyl - L - phenylalanine methyl ester, the precursor of the synthetic sweetener aspartame, was continuously synthesized in an organic solvent with immobilized thermolysin. Ethyl acetate was found to be the most effective organic solvent for the synthesis of this ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/nbt0585-459
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  • 7
    Electronic Resource
    Electronic Resource
    Serial section method for cytofluorometric determinations of the DNA content of component cells of the human cochlea (1987)
    Springer
    Histochemistry and cell biology 87 (1987), S. 115-121 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary We describe a method for measuring the DNA content of the component cells of the organ of Corti using serial sections of human cochleae obtained at autopsy. Cochleae were fixed in Carnoy's solution and embedded in Acrytron E, a water-miscible methacrylate resin. A procedure was developed to reduce the background fluorescence in methacrylate-embedded sections; the resin was pretreated with ion-exchange resin (Amberlite IRA-410). Experiments showed that pretreatment reduce the background fluorescence practically to zero. Seventy 3 μm-thick serial sections were prepared on fluorescence free glass slides and stained with azocarmin G and acriflavine-Feulgen. After postirradiation using blue excitation light, the amount of Feulgen-DNA present in the target nucleus in each section was determined using a microfluorometer. The amount of DNA in the entire nucleus was determined by adding together the DNA contnet of the segments of the nucleus. The characteristic appearance of the organ of Corti made it easy to detect these cells; under green excitation light the cells of this organ exhibited red cytoplasmic azocarmin-G fluorescence. Due to the relatively wide internuclear spaces, cytofluorometry of individual nuclei could be performed without interference from the neighboring cells. Our technique using serial sections allowed us to measure the DNA contnet of individual cells and obtain histological information about particular cells and their neighboring cells. Several polyploid cells were found among the Hensen's cells in the cochlea, while all other component cells of the organ of Corti were diploid.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00533395
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  • 8
    Electronic Resource
    Electronic Resource
    Promoting effect of sodium chloride in 2-stage urinary bladder carcinogenesis in rats initiated by N-Butyl-N-(4-hydroxybutyl)-nitrosamine (1986)
    Springer
    Urological research 14 (1986), S. 201-206 
    Details
    ISSN: 1434-0879
    Keywords: Rat bladder carcinogenesis ; Initiation and promotion ; N-Butyl-N-(4-hydroxybutyl)nitrosamine ; Urinary composition ; Sodium chloride
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The promoting effect of sodium chloride (NaCl) in 2-stage urinary bladder carcinogenesis in F344 rats initiated by 2 doses of N-butyl-N-(4-hydroxybutyl)nitrosamine (BBN) was investigated. The incidences of PN hyperplasia were significantly higher in rats initiated with 0.01 or 0.05% BBN when they were given diet containing 10% NaCl for 32 weeks than when they were given control diet. The incidence of papilloma in rats given 0.05% BBN followed by diet containing 10% or 5% NaCl tended to be higher than that in control rats. The urine of rats given diet containing NaCl was larger in volume and had lower osmolality than that of controls. The total urinary sodium and chloride contents were also increased, whereas those of potassium and phosphorus were decreased. No calculus formation or crystalluria was observed. These data suggest that excess intake of sodium as NaCl has a weak promoting effect in 2-stage urinary bladder carcinogenesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00441114
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  • 9
    Electronic Resource
    Electronic Resource
    Genetic diversity among sublines originating from a single somatic hybrid cell of Duboisia hopwoodii + Nicotiana tabacum (1988)
    Springer
    Theoretical and applied genetics 76 (1988), S. 641-646 
    Details
    ISSN: 1432-2242
    Keywords: Duboisia hopwoodii ; Nicotiana tabacum ; Somatic hybrid ; Nuclear DNA content ; Genetic instability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The genetic instability of an intertribal hybrid cell line, Duboisia hopwoodii + Nicotiana tabacum, obtained by mechanical isolation of a single hybrid cell was studied. Ten subclones of calli derived from this hybrid cell line were cultured for 3 years, and their genetic makeup clarified as to nuclear DNA content, chromosome constitution, and peroxidase isozymes. Nuclear DNA content differed in each subclone. In most subclones, mean DNA content was lower than the mean DNA content in the original hybrid cell line determined 1 year after fusion. This decrease in DNA content is partly attributable to the elimination of tobacco chromosomes that occurred in all subclones. The extent to which tobacco chromosomes were eliminated varied among the subclones — evidence that chromosome elimination occurred slowly. Peroxidase isozyme analysis indicated the loss of a tobacco-specific isozyme, thus confirming results obtained by chromosome analysis. Shoots regenerated from two hybrid subclones after 2 years were also heterogeneous in morphology and nuclear DNA content.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00303507
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  • 10
    Electronic Resource
    Electronic Resource
    Endogenous inhibitor of ecdysone synthesis in crabs (1988)
    Springer
    Cellular and molecular life sciences 44 (1988), S. 50-52 
    Details
    ISSN: 1420-9071
    Keywords: Molt-inhibiting hormone (MIH) ; inhibitor ; ecdysone biosynthesis ; crustaceans ; xanthurenic acid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Attempts to isolate the molt-inhibiting hormone (MIH) of crustaceans from crab eyestalks (ES) resulted in the characterization of xanthurenic acid as an inhibitor of ecdysone biosynthesis in the cultured Y-organ-complex (YOC) homogenate. It was also found that 3-hydroxyl-l-kynurenine present in the ES is transformed into xanthurenic acid in the YOC and body fluid. Its mode of inhibitory action in ecdysone biosynthesis is probably inactivation of cytochrome P-450.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01960242
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