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  • 1
    Electronic Resource
    Electronic Resource
    Biochemical switching device realizing McCulloch-Pitts type equation (1988)
    Springer
    Biological cybernetics 58 (1988), S. 295-299 
    Details
    ISSN: 1432-0770
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Computer Science , Physics
    Notes: Abstract We analyzed cyclic enzyme systems, one of the best candidates for biochemical switching devices, especially focusing on their control mode against external perturbations. Since these systems have the reliability of ON-OFF types of operation (McCulloch-Pitts' neuronic equation), we shall present here the mechanical difference between these systems and electronic switching circuit, especially on the mnemonic mechanism of biochemical switching devices.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00363938
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  • 2
    Electronic Resource
    Electronic Resource
    Free radicals formed by reaction of germane with hydrogen atoms in xenon matrix at very low temperatures (1987)
    College Park, Md. : American Institute of Physics (AIP)
    The Journal of Chemical Physics 86 (1987), S. 4949-4951 
    Details
    ISSN: 1089-7690
    Source: AIP Digital Archive
    Topics: Physics , Chemistry and Pharmacology
    Notes: The radicals formed by the reaction of GeH4 with H in the Xe matrix were investigated by electron spin resonance (ESR) spectroscopy at very low temperatures. The radicals observed were identified as GeH3 and GeH5. The newly observed radical GeH5 is considered as the intermediate of the reaction H+GeH4→H2+GeH3. The ESR parameters of these radicals were determined by numerical deconvolution of the observed spectra.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.452665
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  • 3
    Electronic Resource
    Electronic Resource
    Electronic spin resonance spectra of SiH3 radicals trapped in the low temperature matrix of nonmagnetic isotopes of xenon (1989)
    College Park, Md. : American Institute of Physics (AIP)
    The Journal of Chemical Physics 90 (1989), S. 2992-2994 
    Details
    ISSN: 1089-7690
    Source: AIP Digital Archive
    Topics: Physics , Chemistry and Pharmacology
    Notes: Xenon enriched with nonmagnetic isotopes, in which the content of magnetic isotopes is less than 0.4%, has been used as the low temperature matrix for the observation of electronic spin resonance (ESR) spectra of SiH3 radicals and H atoms. The SiH3 radicals were generated by the reaction of SiH4 with H atoms produced by the photolysis of HI. Well resolved proton hyperfine structures, which were smeared out into a broad singlet spectrum when natural xenon was used as the matrix, were observed. The ESR parameters were determined by numerical deconvolution and compared with those obtained for the radicals trapped in other matrices. Simple doublet spectrum of H atoms without superhyperfine structures was also observed after the photolysis of HI at 4.2 K.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.455900
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  • 4
    Electronic Resource
    Electronic Resource
    Ultrastructural and immunohistochemical studies on the zona-reticularis cells of the adrenal cortex of normal and 3-methylcholanthrene-treated mice (1985)
    Springer
    Histochemistry and cell biology 83 (1985), S. 207-211 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The drug-metabolism activity of adrenocortical cells of normal and 3-methylcholanthrene (MC)-treated ddY mice were examined ultrastructurally and immunohistochemically. Immunoblot analyses performed prior to immunohistochemistry revealed the presence of a protein — possible equivalent to cytochrome P-450 found in the liver microsomes of MC-treated male rats — in the adrenal homogenate of MC-treated female mice. Immunohistochemistry demonstrated the presence of a cytochrome-P-450-like protein in the adrenal-cortical cells (especially in the zona-reticularis cells) of MC-treated female mice; at the same time, a remarkable increase in the amount of SER in these cells was observed by electron microscopy. These findings suggest that cells of the mouse adrenal gland, particularly those of the zona-reticularis, might participate not only in steroid biosynthesis but also in some sort of drug metabolism (detoxication). In addition, there might be a sex-related difference in ddY mice.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00953985
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  • 5
    Electronic Resource
    Electronic Resource
    The role of intracellular Ca2+ in the degranulation of skinned mast cells (1987)
    Springer
    Inflammation research 20 (1987), S. 157-160 
    Details
    ISSN: 1420-908X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The intracellular pH of rat peritoneal mast cells was slightly acidic and compound 48/80 induced a decrease in the cytoplasmic pH of these cells. By means of chemical skinning, it was revealed that perfusion with Ca2+ or inositol 1,4,5-trisphosphate (IP3) induced degranulation dose-dependently in mast cells at concentrations higher than 10 μM and 0.1 μM, respectively. Na+ was essential for the release of histamine from mast cells. An assay based on the binding of45Ca to mast cell fragments revealed that the intracellular Ca store of the mast cell is located in the endoplasmic reticulum. IP3 liberated Ca from the endoplasmic reticulum.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02074656
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  • 6
    Electronic Resource
    Electronic Resource
    Changes in intracellular Ca2+ distribution of rat peritoneal mast cells before and after histamine release (1986)
    Springer
    Inflammation research 18 (1986), S. 61-64 
    Details
    ISSN: 1420-908X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Rat peritoneal mast cells were stained with quin 2, a fluorescent Ca2+ chelator. By means of a fluorescence microscope and real time image processer, it was revealed that the fluorescence derived from the Ca-quin 2 complex was weak in the area occupied by the nucleus and distributed unevenly in the cytoplasm of the resting cells so as to encompass the individual granules. When compound 48/80 or substance P was added in a Ca-free medium, the fluorescence intensity of quin 2 increased markedly all over the cell, suggesting that a large amount of Ca2+ was released from intracellular Ca stores. The increase in the fluorescence intensity produced by compound 48/80 or substance P in a Ca-free medium was inhibited by pretreatment with certain drugs eliciting an increase of c-AMP levels, such as dibutyryl c-AMP and theophylline, or by some anti-allergic drugs providing a membrane stabilizing action.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01987983
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  • 7
    Electronic Resource
    Electronic Resource
    Liquid chromatographic determination of dimethadione and trimethadione in human serum on octadecyl treated microporous glass columns (1987)
    Springer
    Chromatographia 23 (1987), S. 325-327 
    Details
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Packings of octadecylated microporous glass ; Dimethadione and trimethadione in serum ; Pancreatic stones
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary The liquid chromatographic determination of trimethadione (TMO) and its methabolite, dimethadione (DMO) were studied by high-performance liquid chromatography (HPLC) on octadecyl-modified microporous glasses, prepared with toluene solution containing octadecyl-dimethylchlorosilane, using two types of microporous glass with various mean pore diameters and/or specific surface areas. Using acetonitrile-water mixtures as eluents, TMO and DMO in human serum were separated on the glasses studied, but with different degrees of resolution. In this present study, we report the development of a rapid and selective HPLC method for the simultaneous analysis of TMO and DMO in human serum.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02316176
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  • 8
    Electronic Resource
    Electronic Resource
    Study on retention behavior of peropyrene-type polycyclic aromatic hydroccrbons with various bonded stationary phases in reversed-phase liquid chromatography (1988)
    Springer
    Chromatographia 25 (1988), S. 483-486 
    Details
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Reversed-phase diphenyl and naphtylethyl bonded phases ; Retention/molecular polarizability relationships ; Peropyrene aromatics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary The retention behavior of 15 peropyrene-type polycyclic aromatic hydrocarbons was investigated on various bonded stationary phases in reversed-phase liquid chromatography. On diphenyl and naphthylethyl bonded phases, high correlations were obtained between the molecular polarizability of solutes and their retention. However, very low or no correlations were found on various octadecyl bonded phases. These facts are discussed by using the electrostatic interaction concept between the solutes and the stationary phase. We conclude that these observations are due to two reasons: the difference in the degree of planarity of polycyclic aromatic hydrocarbons and the high ability of planarity recognition of octadecyl bonded phases.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02324817
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  • 9
    Electronic Resource
    Electronic Resource
    Compatibility and universality of the retention prediction system for polycyclic aromatic hydrocarbons in reversed-phase liquid chromatography (1986)
    Springer
    Chromatographia 21 (1986), S. 24-28 
    Details
    ISSN: 1612-1112
    Keywords: Column-liquid chromatography ; Retention prediction system ; Reversed-phase ; Compatibility ; Polycyclic aromatic hydrocarbons
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary System-compatibility and universality of the retention prediction concept has been investigated for polycyclic aromatic hydrocarbons in reversed-phase liquid chromatography. The results clearly indicate that the retention prediction approach has a very high potential for optimization of separation conditions in almost all reversed-phase systems, and allows more precise and rapid analysis. This approach may be one of the best optimization techniques, because the system does not require any standard materials.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02310590
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  • 10
    Electronic Resource
    Electronic Resource
    Determination of chenodeoxycholic acid on 3-cyanopropyltrichlorosilane-treated high-performance thin-layer chromatographic plates (1987)
    Springer
    Chromatographia 23 (1987), S. 601-603 
    Details
    ISSN: 1612-1112
    Keywords: High-performance, thin-layer chromatography (HPTLC) ; 3-Cyanopropyltrichlorosilane-treated plates ; Chenodeoxycholic acid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary Chenodeoxycholic acid in commerical drugs was analyzed by high-performance, thin-layer chromatography (HPTLC) using a cyanoalkyl chemically-bonded stationary phase, prepared by treating pre-coated silica with 3-cyanopropyltrichlorosilane (3CPTS). The 3CPTS-treated plates were used to evaluate commerical chenodeoxycholic acid in drug capsules. After development with methanol on the 3CPTS-treated plate, the chenodeoxycholic acid spot in the chromatogram was measured with a TLC densitometer equipped with a dual-wave length TLC scanner at λ=370nm. By this method, the fluorescence intensity of chenodeoxycholic acid was measured within 2.6% error over the range 30–240ng and the limit of detection was 30ng.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02324873
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