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  • 1
    Electronic Resource
    Electronic Resource
    Influence of conventionalization on cecal wall structure of germ-free Wistar rats: quantitative light and qualitative electron microscopic observations (1989)
    Springer
    Anatomy and embryology 180 (1989), S. 191-198 
    Details
    ISSN: 1432-0568
    Keywords: Cecum ; Germ-free rat ; Microflora inoculation ; Morphometry ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The structural changes of the cecal wall in germfree rats were observed at regular intervals after the inoculation of fecal microflora from conventional rats. Quantitative light microscopy showed that most of the elements in the cecal wall increased at 12 or 24 h and reached peak values at 4 days after inoculation. On the 7th day, they decreased approximately to the values for conventional rats. The crypts were bent or widely open till 24 h but were not after the 4th day. Hyperplasia of the crypt epithelial cells including mucous-type cells was observed following microbial inoculation. Electron microscopy revealed that most of the epithelial cells lining the mucosa were typical columnar cells. Desquamation of the epithelial cells and contraction of the muscle fibers were often seen on 4th day. The mucous-type cells were divided into two types, goblet and non-goblet mucous-type cells. Reduction of cecal volume after microbial inoculation may be mainly caused by muscle contraction in the early period and hyperplasia and desquamation of the epithelial cells may suggest their role as the first and non-specific defense line prior to operation of the specific immune system.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00309771
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  • 2
    Electronic Resource
    Electronic Resource
    Electron microscopic study on brain of macular mutant mouse after copper therapy (1988)
    Springer
    Acta neuropathologica 76 (1988), S. 574-580 
    Details
    ISSN: 1432-0533
    Keywords: Macular mouse ; Menkes kinky hair disease ; Copper therapy ; Mitochondrial abnormalities
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The hemizygote of the macular mutant mice, which is clinically and neuropathologically considered to be a model of Menkes kinky hair disease (MKHD), were injected intraperitoneally four times with 10, 20, 20 and 30 μg of cupric chloride on days 4, 6, 8 and 10 after birth, respectively. Their cerebral and cerebellar cortices were chronologically examined by electron microscopy. In the cerebral cortes, only a few abnormal mitochondria with electron-lucent matrix and short peripherally located cristae were scattered in the neurons on day 14, and these had almost entirely vanished after day 21. In the cerebellar cortex, abnormal mitochondria were frequently found on day 14 in the dendrites of the Purkinje cells, whereas they were only occasionally observed in their cytoplasm. Those in the dendrites had decreased in number on day 30, and only a few of them were seen in the cerebellum after day 45. These results show that the copper therapy reduced ultrastructural abnormalities in the hemizygote of this mutant mouse.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00689595
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  • 3
    Electronic Resource
    Electronic Resource
    Contents and Retention of Nutrients in Extra Lean, Lean and Regular Ground Beef (1985)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 50 (1985), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Contents and retention of nutrients in raw and cooked (broiled, roasted and grilled to medium-well stage) ground beef patties were established. Fat levels of the raw patties were 18.4, 21.5, and 27.0%. Cooking yields were slightly lower and energy contents were higher for patties containing 27.0% fat than 18.4 or 21.5%. Total lipids, energy content, thiamin and pantothenic acid were the least retained nutrients while zinc was the highest. Generally, retentions after broiling or grilling were the same but were higher than after roasting. The polyunsaturated to saturated fatty acid ratios (P/S) rose slightly after cooking. For most nutrients, differences due to fat levels or cooking methods appear to be too small to be of practical nutritional significance.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.1985.tb13776.x
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  • 4
    Electronic Resource
    Electronic Resource
    Assessment of Methodologies for Calorimetric Cholesterol Assay of Meats (1988)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 53 (1988), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The objective of this study was to assess the critical factors influencing the values for meat cholesterol determined colorimetrically. Procedure variations tested included: no saponification of total lipid extract before color development; with 15 min saponification at 80°C, with or without antioxidant protection; and with 60 min saponification at 80°C, with or without antioxidant protection. Without the saponification step, meat cholesterol values were overestimated. Samples with a large percentage of unsaturated fatty acids yielded higher cholesterol values when lipid extracts were saponified without antioxidant protection than with. Meat cholesterol values obtained by the procedure involving 15 min saponification with antioxidant protection were similar to those obtained by a commonly used gas chromatographic procedure.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.1988.tb07804.x
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  • 5
    Electronic Resource
    Electronic Resource
    Impact of Sodium Ascorbate and Sodium Erythorbate Used in Meat Processing on the Vitamin B12 Contents of Cured Ham (1989)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 54 (1989), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Effects of sodium ascorbate (Na ascorbate) and sodium erythorbate (Na erythorbate) on vitamin B]2 content of cured, cooked ham were investigated. Paired hams were injected with cure solution containing no curing accelerators or with cure solution containing Na ascorbate or Na erythorbate (3.28, 6.56, 9.84 g/L). There were no differences between vitamin B12 contents of hams containing added curing accelerators and paired controls, indicating that B12 was not destroyed by either Na ascorbate or Na erythorbate. Analysis of ascorbate, dehy-droascorbate, isoascorbate and dehydroisoascorbate showed ascorbate to be the only substance present in Na ascorbate-treated hams and isoascorbic acid to be the only substance present in Na erythorbate-treated hams.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.1989.tb05138.x
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  • 6
    Electronic Resource
    Electronic Resource
    Nutrient Composition of Some Fresh and Cooked Retail Cuts of Veal (1986)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 51 (1986), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: In order to account for changes in veal production technology and to provide more details than presently available, nutrient composition of the lean from milk-fed calves (Bob) and special fed veal (SFV) were determined. Values for proximate composition, cholesterol, 8 fatty acids, 8 vitamins, and 8 inorganic nutrients were obtained. Bob veal tended to have lower fat content and significantly (P〈0.05) higher levels of cholesterol and Fe than SFV. Percent true retentions were above 100% for fat, cholesterol and energy content. Bob and SFV are good sources for niacin, vitamin B12, P and Zn, but contain slightly higher amounts of cholesterol than literature values for beef, pork or lamb.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.1986.tb13120.x
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  • 7
    Electronic Resource
    Electronic Resource
    Generation of pure ion beams by field ionization of laser-excited Rydberg atoms (1988)
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 64 (1988), S. 1619-1624 
    Details
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: Sodium atoms (108–1011 cm−3) effused from an oven were stepwise excited from their ground state to a Rydberg state ns or nd (20≤n≤26) by two pulsed dye lasers (∼5–10 ns, ∼100 μJ, ∼1.6-mm-diam spot). The laser energy density necessary to saturate the excitation processes from the ground state to the Rydberg state is ∼ several mJ/cm2. About 50 ns after the laser irradiation, a pulsed electric field (0.5–5.5 kV/cm) was applied to the Rydberg atoms to ionize them and accelerate the resulting ions. Thus, a pulsed sodium ion beam of purity 〉99.99% was obtained with a maximum total electric charge of ∼5 pC and a pulse width of ∼120 ns full width at half maximum. The total electric charge of the ion beam is estimated including the effect of space-charge field of the beam ions, and is in good agreement with the experimental values.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.341809
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  • 8
    Electronic Resource
    Electronic Resource
    Transcriptional regulation of F plasmid gene srnB: rifampicin-promoted in vitro readthrough of a terminator in the leader region (1989)
    Oxford, UK : Blackwell Publishing Ltd
    Molecular microbiology 3 (1989), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: srnB is an F-plasmid encoded gene, otherwise silent, whose expression is induced by added rifampicin, leading to the release of cellular Mg2+ and degradation of stable RNA. In the absence of rifampicin, transcripts from the srnB gene were relatively short. S1 nuclease mapping revealed that the short mRNA species terminated within the leader, at the 3’end of a potential stem-and-loop structure. A deletion in the stem-loop resulted in constitutive synthesis of the mRNA that extended beyond the termination site into the structural gene. Even with the wild-type gene, transcription continued beyond the terminator sequence in the presence of added rifampicin. Most of the transcripts synthesized in the presence of rifampicin were long enough to encode the srnB protein. We hypothesize from these results that RNA polymerase associated with rifampicin can read through the terminator to induce srnB expression.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2958.1989.tb00227.x
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  • 9
    Electronic Resource
    Electronic Resource
    Early-time behavior of spectral emissions from the interaction of intense relativistic electron beams with initially neutral gases (1988)
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 63 (1988), S. 4392-4396 
    Details
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: Visible-UV spectral emissions from electron beam (700 kV, 12 kA, 50 ns) generated neon plasmas have been investigated over a wide pressure range (0.1–100 Torr) with and without axial magnetic guide field. The Ne I–III line emissions were viewed perpendicular to the beam propagation; attention was focused on a time delay of the onset of the emission with respect to the beam front arrival in the field of view, and on correlations of the subsequent intensity evolution with that of the beam and net (beam plus plasma) currents. The observations are interpreted as direct confirmation of the major processes for gas ionization and excitation that occur depending on the gas pressure and magnetic guide field.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.340182
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  • 10
    Electronic Resource
    Electronic Resource
    Replication of rat coronavirus in a rat cell line, LBC (1985)
    Springer
    Archives of virology 85 (1985), S. 301-304 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Rat coronavirus readily propagated and induced marked cytopathic effect in a rat cell line, LBC cell culture, which provided a sensitive, practical assay system for viral infectivity and neutralizing antibody, and a satisfactory source of the virus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01314238
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