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  • 1985-1989  (3)
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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Clinical & experimental allergy 15 (1985), S. 0 
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: In the present study, it was shown that allergen challenge in vitro produced an increase in the number of antigen-specific IgE-plaque-forming cells of peripheral-blood lymphocytes from grass- or ragweed-allergic patients. Thus, the blood lymphocytes of all twelve (four rye grass I and eight AgE) sensitive donors responded whereas the blood lymphocytes of five non-atopic controls were unresponsive to antigen challenge. Allergen challenge doses of 10−10−10−12 g/ml were found to give the greatest number of plaque-forming cells whereas the number of plaque-forming cells at challenging doses between 10−9 and 10−7 g/ml were either the same or less than those obtained with unchallenged cells. The results are discussed as to whether this in vitro model system represents in vivo response to allergen of the allergic patient.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Clinical & experimental allergy 15 (1985), S. 0 
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The reverse haemolytic plaque assay was developed to measure antigen (AgE or rye grass I)-specific IgE-plaque-forming cells from the peripheral-blood lymphocytes of ragweed- and grass-allergic patients. The anti-IgE-developing antisera was shown to be isotype-specific, and the response of the assay was inhibited by 52% by the addition of 10 pg of antigen. In addition, the assay was shown to have a reproducibility (s.d.) of 15%. The blood lymphocytes from all fifteen atopic (grass and ragweed) patients were shown to form antigen-specific IgE-plaque-forming cells during the pollen season (mean value 115 cells) and up to 6 months after the season (mean value 56 cells). Cycloheximide appears to block the formation of the plaque-forming cells. This method appears to be sensitive and reproducible enough to study in vitro IgE antibody synthesis of peripheral-blood lymphocytes from atopies.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1434-601X
    Keywords: 25.55.−e ; 25.55 Ek ; 25.10.+s
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Experiments on the reaction:3He+3He→4He+2He using a single four detector telescope have shown evidence for the production of deuteron pulses, consistent with the2He→2H+e++v fusion reaction in the final state. New measurements on the same reaction using improved techniques viz.A) a windowless3He gas target andB) α- d correlation detection with 5 ns resolving time and antipileup circuitry have been performed. Calibrations were obtained from the7Li+3He→2H+4He+4He reaction spectra. The correlation angles were 30° and 97° for the alphas and deuterons respectively. Deuteron pulses have been observed, correlated with alpha particles. A cross section of (1.7 ±0.5)nbsr−1MeV−1 was obtained consistent with previous measurements using a single telescope.
    Type of Medium: Electronic Resource
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