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  • 1985-1989  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 147 (1987), S. 88-91 
    ISSN: 1432-072X
    Keywords: Aspergillus niger ; ATP:citrate lyase ; Citric acid accumulation ; Lipid biosynthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract ATP:citrate lyase (EC 4.1.3.8) has been identified in cell-free extracts from the filamentous fungus Aspergillus niger. The enzyme was located in the cytosol. It exhibits an activity at least ten times that of acetate-CoA-kinase (EC 6.2.1.1) during growth on carbohydrates as carbon sources, and is thus considered responsible for acetyl-CoA formation under these conditions. It is formed constitutively and its biosynthesis does not appear to be controlled by changes in the nitrogen or carbon source or type. ATP:citrate-lyase appears to be very labile during conventional purification procedures; a method involving fast protein liquid anion exchange chromatography was thus developed in order to obtain enzyme preparations sufficiently free of enzymes which could interfere with kinetic investigations. This preparation displays commonly known characteristics of ATP:citrate lyase with respect to substrate affinities and cofactor requirements, with the exception that the affinity for citrate is rather low (2.5 mM). No activator was found. The enzyme is inhibited by nucleoside diphosphates, nucleoside monophosphates and palmitoyl-CoA. Regulation of ATP:citrate lyase be the energy charge of the cytosol in relation to lipid or citric acid accumulation is discussed in view of these findings.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    The European physical journal 331 (1988), S. 43-51 
    ISSN: 1434-601X
    Keywords: 24.10.Cn ; 24.60 ; 25.70
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract We show how the fluctuating part of the residual coupling between collective and intrinsic motion of a dissipative heavy-ion collision induces correlations in either subspace. They lead in general to a transport equation for the collective motion, and to a new term in the equation for the one-body density which describes collisions with the collective fluctuations. The resulting redistribution of the single-particle occupation numbers ρα and the evolution of the fluctuations are coupled with each other due to the dependence of the transition rates in the master equation on the fluctuations, and of the transport coefficients on ρα. Considering the special case of a long contact phase, we find the fluctuations to be most effective, with respect to a randomization of ρα, within a certain critical region where they pass from stable to unstable behaviour. Estimates are made for the corresponding relaxation times employing a schematic model.
    Type of Medium: Electronic Resource
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