ISSN:
0148-7280
Keywords:
glycoprotein
;
immunocytochemistry
;
protein A-colloidal gold
;
embryo cortex
;
Life and Medical Sciences
;
Cell & Developmental Biology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
Notes:
The intracellular and cell surface localization of an embryonic glycoprotein antigen (BL) has been investigated in preimplantation mouse embryos using ultrastructural immunocytochemistry. Several interesting points have emerged: (1) BL antigens are exclusively localized subjacent to the plasma membrane in the cortical region of cells, whereas antigens detected by a control antibody against mouse L cells are distributed throughout the embryo. (2) The distribution of BL antigens is polarized beginning with the first cleavage, with expression confined to the cortex underlying the free or apical portions of cells. No antigen is present underlying regions of cell contact. (3) Although embryonic synthesis of BL antigens does not begin until the two-cell stage, BL antigens are observed in unfertilized eggs, a fact verified by immunoblotting.
Additional Material:
9 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/mrd.1120120402
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