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  • 1
    Electronic Resource
    Electronic Resource
    Effect of ligation of common bile duct on somatostatin levels and binding in cytosol of rabbit duodenal mucosa (1986)
    Springer
    Bioscience reports 6 (1986), S. 1077-1083 
    Details
    ISSN: 1573-4935
    Keywords: bile duct ; duodenal mucosa ; somatostatin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Rabbits with ligation of the common bile duct, of one and three weeks duration, showed a significant increase of somatostatin content in duodenal mucosa and plasma as compared with control animals. The increase of mucosal somatostatin was associated with a decrease in the binding capacity of both high- and low-affinity binding sites without changes in the affinity values in cytosol of duodenal mucosa. These findings suggest that the number of somatostatin binding sites is inversely related to local levels of the peptide and support the hypothesis of somatostatin regulating its own binding sites.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01141030
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Specific somatostatin-binding to cytosol of bovine gallbladder mucosa (1986)
    Springer
    Bioscience reports 6 (1986), S. 283-291 
    Details
    ISSN: 1573-4935
    Keywords: somatostatin ; gallbladder ; mucosa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The binding of somatostatin was studied in the cytosolic fraction of bovine gallbladder mucosa. The binding reaction depended on time, temperature and pH, and was reversible, saturable and specific. Stoichiometric data suggested the presence of two classes of binding sites: a class with high affinity (K d=23.6 nM) and low capacity (3.7 pmol somatostatin/mg protein) and a class with low affinity (K d=284.6 nM) and high capacity (85.0 pmol somatostatin/mg protein) at 37°C and pH 7.4. The binding sites were highly specific for somatostatin since peptides such as [Leu]enkephalin, neurotensin, vasoactive intestinal peptide and substance P showed practically no effect upon somatostatin binding. The presence of somatostatin-binding sites in the cytosolic fraction of gallbladder mucosa, together with the known occurrence of somatostatin nerve endings in the gallbladder strongly suggests that this peptide may be involved in the physiology and physiopathology of gallbladder mucosa.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01115157
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  • 3
    Electronic Resource
    Electronic Resource
    Effects of alloxan-induced diabetes on somatostatin binding to cytosolic components of rabbit gastric fundic mucosa (1988)
    Springer
    Bioscience reports 8 (1988), S. 233-240 
    Details
    ISSN: 1573-4935
    Keywords: somatostatin binding ; gastric mucosa ; alloxan ; diabetes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Diabetes was induced by administration of alloxan (150 mg/Kg) to 24 h-fasted rabbits. Somatostatinlike immunoreactivity (SLI) and cytosolic binding sites for somatostatin in gastric fundic mucosa were studied using radiolabelled Tyr11-somatostatin. Three months after the onset of the disease, the specific binding of somatostatin to these sites was seen to be significantly lower, due to a reduction in the number (but not the affinity) of specific somatostatin binding sites of high-affinity and a disappearance of the specific, somatostatin binding sites of low-affinity. These changes were associated with an increase in the SLI concentration in both gastric fundic mucosa and plasma.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01115040
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  • 4
    Electronic Resource
    Electronic Resource
    Characterization and age dependence of the stimulatory effect of VIP on cyclic AMP accumulation in rat Leydig cells (1987)
    Springer
    Bioscience reports 7 (1987), S. 805-811 
    Details
    ISSN: 1573-4935
    Keywords: VIP ; cyclic AMP ; development ; Leydig cell
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The neuropeptide vasoactive intestinal peptide (VIP) has been shown to stimulate cyclic AMP accumulation in Leydig cells isolated from rat testis. The effect was dependent on time, temperature and cell concentration. At 15° half-maximal and maximal stimulation were observed at about 1 and 100 nM VIP, respectively. The interaction was specific since an order of potencies chicken VIP〉 rat VIP〉 secretin〉glucagon and no effect of neurotensin and substance P were obtained. The efficiency of VIP was lower in pubertal rats and then increased in young-adult and adult animals. These results together with the known presence of VIP in the testis support the idea that VIP may be involved in the regulation and function of Leydig cells during development.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01116754
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  • 5
    Electronic Resource
    Electronic Resource
    The effects of experimental uremia in rats on duodenal VIP levels and the interaction of VIP with duodenal epithelial cells (1989)
    Springer
    Bioscience reports 9 (1989), S. 207-212 
    Details
    ISSN: 1573-4935
    Keywords: VIP ; cyclic AMP ; uremia ; enterocyte
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The effects of experimental uremia on the concentration of vasoactive intestinal peptide (VIP) in duodenum as well as on the interaction of this neuropeptide with the corresponding epithelial cells were studied in rats. Duodenal VIP concentration was significantly decreased in uremic rats as compared to control animals. The specific binding of VIP to duodenal epithelial cells increased in rats with uremia due to an increase in the number of VIP receptors rather than a change in the binding affinity or in the extent of VIP degradation. On the other hand, the efficacy but not the potency of VIP upon cyclic AMP generation varied in parallel to that observed at the receptor level.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01115997
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  • 6
    Electronic Resource
    Electronic Resource
    Characterization of insulin receptors in isolated epithelial cells of rat ventral prostate: Effect of fasting (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    Cell Biochemistry and Function 4 (1986), S. 19-24 
    Details
    ISSN: 0263-6484
    Keywords: Insulin ; peptide hormone receptor ; prostatic epithelial cells ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Insulin receptors have been characterized in rat prostatic epithelial cells by using [125I]insulin and a variety of physicochemical conditions. The binding data at equilibrium (2h at 15°C) could be interpreted in terms of two populations of insulin receptors: a class of receptors with high affinity (Kd = 2·16 nM) and low binding capacity (28·0 fmol mg-1 protein), and another class of receptors with low affinity (Kd = 0·29 μM) and high binding capacity (1·43 pmol mg-1 protein). Proinsulin exhibited a 63-fold lower affinity than insulin for binding sites whereas unrelated peptides were ineffective. The specific binding of insulin increased by about 50 per cent after 96 h of fasting; this increase could be explained by an increase of both the number of the high affinity-low capacity sites and the affinity of the low affinity-high capacity sites. These results together with previous studies on insulin action at the prostatic level strongly suggest that insulin may exert a physiological role on the prostatic epithelium.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cbf.290040103
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    Paper (German National Licenses)
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  • 7
    Electronic Resource
    Electronic Resource
    Growth hormone binding and stimulation of amino acid uptake in epithelial cells of rat ventral prostate (1987)
    New York, NY [u.a.] : Wiley-Blackwell
    Cell Biochemistry and Function 5 (1987), S. 63-68 
    Details
    ISSN: 0263-6484
    Keywords: Growth hormone ; peptide hormone receptor ; prostatic epithelial cells ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The binding of [125I]-human growth hormone (hGH) was studied in epithelial cells isolated from rat ventral prostate. Binding and degradation were dependent on time and temperature. The effect of a lysosomotropic agent suggested internalization and lysosomal degradation of the hormone. Dissociation and stoichiometric studies indicated the existence of a single class of GH receptors with a Kd of 0·7 nM and a binding capacity of 46 fmol hGH bound mg-1 cell protein. The receptor appeared to possess a somatotrophic nature since lactogenic hormones such as human placental lactogen and rat prolactin exhibited a very low degree of competition (whereas a variety of unrelated hormones and neuropeptides showed no effect). GH-stimulated leucine uptake by the cells in a time- and dose-dependent manner, half maximal effect being observed at 0·32 nM GH thus suggesting a direct relationship with the binding step.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cbf.290050108
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    Paper (German National Licenses)
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