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  • 1
    Electronic Resource
    Electronic Resource
    Movement and segregation of kinetochores experimentally detached from mammalian chromosomes (1988)
    [s.l.] : Nature Publishing Group
    Nature 336 (1988), S. 251-254 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] When Chinese hamster cells were arrested at the Gl/S boun-dary of the cell cycle by treatment with 2 mM hydroxyurea for 20 h and subsequently treated for 4-6 h with caffeine (5 mM), the cells entered mitosis without completing DNA synthesis as originally observed in BHK (baby hamster kidney) ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/336251a0
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  • 2
    Electronic Resource
    Electronic Resource
    Trisomic categories in pearl millet (Pennisetum americanum (L.) Leeke) (1988)
    Springer
    Theoretical and applied genetics 75 (1988), S. 340-343 
    Details
    ISSN: 1432-2242
    Keywords: Pearl millet ; Primary ; Secondary ; Tertiary trisomy ; Interchanges
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In pearl millet [Pennisetum americanum (L.) Leeke], in the open pollinated and crossed progenies of autotriploids, desynaptics and translocation heterozygotes, two primary trisomics, one each of secondary and tertiary trisomics, two primary trisomics with interchanges, two interchange secondary trisomics, and three interchange tertiary trisomics were located. These categories were determined on the basis of chromosomal associations formed at meiosis. In one other trisomic, its category, whether tertiary or interchange trisomy, could not be determined. Some of these categories, like the secondary trisomy and interchange tertiary trisomy, are reported for the first time.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00303974
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  • 3
    Electronic Resource
    Electronic Resource
    A study of morphology, cytology and sterility in interspecific hybrids and amphidiploids of Nicotiana knightiana X N. umbratica (1985)
    Springer
    Theoretical and applied genetics 70 (1985), S. 330-332 
    Details
    ISSN: 1432-2242
    Keywords: Nicotiana knightiana ; N. umbratica ; Interspecific hybrids ; Cytology ; Female sterility
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Interspecific hybrids and amphidiploids of Nicotiana knightiana Goodspeed (n= 12)x N. umbratica Burbidge (n = 23) resembled either parent in some characters and were intermediate in other characters. The F1 hybrids (2n = 35) showed mostly univalents during meiosis, while the amphidiploids (2n = 70) formed bivalents almost regularly. The former were completely sterile and the latter fully male fertile but predominantly female sterile. This female sterility was due to disintegration of the embryo sacs leading to collapsed ovules. The few fertile ovules, however, showed normal development of embryo sac and embryo. The occurrence of fertile and sterile ovules was believed to be due to segregation of the genes governing sterility.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00304921
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  • 4
    Electronic Resource
    Electronic Resource
    Distribution of phosphorylated spindle-associated proteins in the diatom Stephanopyxis turris (1989)
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 12 (1989), S. 33-41 
    Details
    ISSN: 0886-1544
    Keywords: phosphorylation ; MPM-2 ; mitotic spindle ; microtubule-associated protein ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Mitotic spindles isolated from the diatom Stephanopyxis turris become thiophosphorylated in the presence of ATPγS at specific locations within the mitotic apparatus, resulting in a stimulation of ATP-dependent spindle elongation in vitro. Here, using indirect immunofluorescence, we compare the staining pattern of an antibody against thiophosphorylated proteins to that of MPM-2, an antibody against mitosis-specific phosphoproteins, in isolated spindles. Both antibodies label spindle poles, kinetochores, and the midzone. Neither antibody exhibits reduced labeling in salt-extracted spindles, although prior salt extraction inhibits thiophosphorylation in ATPγS. Furthermore, both antibodies recognize a 205 kd band on immunoblots of spindle extracts. Microtubule-organizing centers and mitotic spindles label brightly with the MPM-2 antibody in intact cells. These results show that functional mitotic spindles isolated from S. turris are phosphorylated both in vivo and in vitro. We discuss the possible role of phosphorylated cytoskeletal proteins in the control of mitotic spindle function.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cm.970120105
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