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  • 1
    Electronic Resource
    Electronic Resource
    Are carotenoids the blue-light photoreceptor in the photoinduction of protoperithecia in Neurospora crassa? (1986)
    Springer
    Planta 168 (1986), S. 56-60 
    Details
    ISSN: 1432-2048
    Keywords: Albino mutant ; Ascomycetes ; Carotenoid ; Blue light ; Mutant (Neurospora) ; Neurospora ; Photoreceptor (blue light) ; Protoperithecia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A triple albino mutant of Neurospora crassa with a measured content of carotenoids absorbing at 470 nm less than 0.5% of that of the wild type (calculated value less than 8·10-4%) had the same threshold for photoinduction of protoperithecia as the wild type when illuminated with monochromatic light at 471 nm. This is strong evidence against the hypothesis that the bulk of carotenoids are the blue-light photoreceptor for this phenomenon. However, it is impossible to exclude traces of carotenoids acting as the photoreceptor at less than 3·10-12 M in a very efficient sensory transduction chain.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00407009
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  • 2
    Electronic Resource
    Electronic Resource
    Role of nitrogen in the photoinduction of protoperithecia and carotenoids in Neurospora crassa (1987)
    Springer
    Planta 170 (1987), S. 205-208 
    Details
    ISSN: 1432-2048
    Keywords: Ascomycetes ; Blue light ; Carotenoid ; Light and carotenoid induction ; Light and protoperithecia induction ; Neurospora ; Nitrogen and photoinduction ; Protoperithecium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Nitrogen, as KNO3 or NH4NO3, can inhibit the photoinduction of protoperithecia in Neurospora crassa when present in the medium at a high concentration but does not inhibit the photoinduction of carotenoids. The point at which the presence of high nitrogen levels is no longer inhibitory is 5 h after illumination.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00397889
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Visualization of halos in the epidermal cell wall of Allium cepa caused by Colletotrichum dematium f. circinans and Botrytis allii using fluorochromes (1987)
    Springer
    Mycopathologia 97 (1987), S. 137-141 
    Details
    ISSN: 1573-0832
    Keywords: fungal-induced halo ; fluorochromes for fungi
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Halos were detected with epifluorescence microscopy around penetration sites of Colletotrichum dematium f. circinans and Botrytis allii in onion epidermal cell walls as areas of less intense fluorescence or negatively stained areas in fluorescing cell walls following treatments with berberin sulphate and acridine orange but not with brilliant sulphaflavine (which stained the cell wall), ninhydrin, dansylchloride, or analine blue. Since pectin, pectic acid, avacil (microcrystaline cellulose super fine), filter paper, and Sephadex G-100–120 fluoresced with acridine orange and berberin sulphate, it was inferred that the halos were negatively stained or appeared as areas with less intense fluorescence because enzymes from these pathogens degraded cell wall pectin and cellulose at the point of penetration. Spores of both pathogens fluoresced when stained with brilliant sulphaflavine, acridine orange, ninhydrin, and dansylchloride. These stains and berberin sulphate caused germ tubes, appressoria, and primary infection mycelia to fluoresce. Nuclei in these fungal structures fluoresced when stained with acridine orange and brilliant sulphaflavine.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00437236
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  • 4
    Electronic Resource
    Electronic Resource
    Degenerative changes in the epidermal cell wall of Allium cepa caused by Botrytis allii: Loss of dry mass, pectin, and cellulose in halos (1988)
    Springer
    Mycopathologia 101 (1988), S. 47-52 
    Details
    ISSN: 1573-0832
    Keywords: Botrytis allii ; Allium cepa ; halo composition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Halos detected using interference microscopy (even- and fringle-field modes with monoand poly-chromatic light) around penetration sites of Botrytis allii in cell walls of normal and protoplast-free outer epidermal tissue of white, yellow, and red onions were alike. Halos in protoplast-free cell walls contained 33% less dry mass than areas of these walls adjacent to halos (quantitative interference miscroscopy with 546 nm light in the even-field mode). Halos were significantly larger in the white onion than in the yellow and red varieties. The loss of cell wall dry mass during the production of halos involved the loss of pectin and cellulose. We infer that this is caused by enzymes released from the pathogen. Cuticle degradation at penetration sites was not observed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00455668
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  • 5
    Electronic Resource
    Electronic Resource
    Protoplast responses in the epidermis of Allium cepa induced by penetration by Botrytis allii (1988)
    Springer
    Mycopathologia 102 (1988), S. 169-173 
    Details
    ISSN: 1573-0832
    Keywords: Allium cepa ; Botrytis allii ; penetration responses
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Penetration of Allium cepa epidermal cells (white, yellow, and red varieties) by Botrytis allii induced a response by host protoplasts in normal tissue which was not observed when penetrations were made in protoplast-free host cell walls. Callose and auto-fluorescing substances (possibly phenolic compounds) were located at the penetration sites only in normal host cells containing protoplasts. Lignin tests were negative. Halos were clearly visible in both types of tissue. Autofluorescence was observed at penetration sites in normal cells of all cultivars but general wall background autofluorescence was not observed in white onions. Autofluorescence was generally yellow green and when treated with ammonium hydroxide became green. Treatment with sodium hydroxide abolished autofluorescence. No attempt was made to isolate the autofluorescing material.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00437400
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  • 6
    Electronic Resource
    Electronic Resource
    Inorganic nutrition of Fusarium oxysporum f. sp. lycopersici and Rhizoctonia solani (1985)
    Springer
    Mycopathologia 89 (1985), S. 123-126 
    Details
    ISSN: 1573-0832
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Fusarium oxysporum f. sp. lycopersici and Rhizoctonal solani were grown in a complete 1.0 mM nutrient solution, and in solutions where Ca, Fe, K, Mg, N, P, and S were either excluded (0.0 mM) or included at depleted levels (0.1 mM) while all other constituents were maintained at 1.0 mM levels. Dry weight of both fungi were determined. For both fungi some of the lowest dry weights were recorded for samples grown in the complete solution. Exclusion of K, Mg, and S significantly increased dry weight of Fusarium. Inclusion at the 0.1 mM level of most components significantly increased Fusarium dry weight over values for both the complete and corresponding excluded nutrient solutions. The exception was S where there was no difference between excluded and 0.1 mM solutions. For Rhizoctonia dry weights in Fe excluded solutions were less than the complete solution, while dry weights in S excluded solutions were greater than the complete solution. At the 1.0 mM level Fe, K, and Mg dry weights were significantly increased over the dry weights produced in both the complete and deficient solutions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00431481
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