ISSN:
1573-6776
Source:
Springer Online Journal Archives 1860-2000
Topics:
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Summary Friable calli and cell suspension cultures were obtained from leaf segments ofEuphorbia characias L. subsp.characias, in Murashige and Skoog (MS) basal medium supplemented with 1g.l−1 casein hydrolyzate (CH), 5mg.l−1 ascorbic acid, 1.0mg.l−1 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.75mg.l−1 benzyl adenine (BA). The highest callus specific growth rate (μ=0.085.day−1), calculated for 1 year old calli cultures, was obtained with 0.25 mg.l−1 2,4-D and 0.50mg.l−1 BA. Suspension cultures started with an inoculum of 8.0×104 cells.ml−1 in supplemented liquid MS medium, gave a specific growth rate μ=0.256.day−1.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01031574
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