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  • 1
    Electronic Resource
    Electronic Resource
    Prosomes (1987)
    Springer
    Molecular biology reports 12 (1987), S. 232-232 
    Details
    ISSN: 1573-4978
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00356915
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Cytoplasmic poly(ADP-ribose) polymerase and poly(ADP-ribose) glycohydrolase in AEV-transformed chicken erythroblasts (1988)
    Springer
    Molecular biology reports 13 (1988), S. 35-44 
    Details
    ISSN: 1573-4978
    Keywords: poly(ADP-ribose) polymerase ; poly(ADP-ribose) glycohydrolase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Poly(ADP-ribose) polymerase and poly(ADP-ribose) glycohydrolase activities were both investigated in chicken erythroblasts transformed by Avian Erythroblastosis Virus. Respectively 21% and 58% of these activities were found to be present in the post-mitochondrial supernatant (PMS). Fractionation of the PMS on sucrose gradients and poly(A+) mRNA detection by hybridization to [3H] poly(U) show that cytoplasmic poly(ADP-ribose) polymerase is exclusively localized in free mRNP. The glycohydrolase activity sedimented mostly in the 6 S region but 1/3 of the activity was in the free mRNP zone. Seven poly(ADP-ribose) protein acceptors were identified in the PMS in the Mr 21000–120000 range. The Mr 120000 protein corresponds to automodified poly(ADP-ribose) polymerase. A Mr 21000 protein acceptor is abundant in PMS and a Mr 34000 is exclusively associated with ribosomes and ribosomal subunits. The existence of both poly(ADP-ribose) polymerase and glycohydrolase activities in free mRNP argues in favour of a role of poly(ADP-ribosylation) in mRNP metabolism. A possible involvement of this post translational modification in the mechanisms of repression-derepression of mRNA is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00805637
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Correlations of repetitive and AT-rich DNA segments within the chicken globin gene domains (1986)
    Springer
    Molecular biology reports 11 (1986), S. 177-187 
    Details
    ISSN: 1573-4978
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The repetitive DNA segments were mapped within a 30 Kbp genomic domain including (in 5′ to 3′ order) the chicken embryonic pi and adult alpha D (minor) and alpha A (major) globin genes. Two repeats map 5 and 8 Kbp upstream from the embryonic pi gene and another 3 Kbp downstream of the adult alpha A gene. These repetitive DNA sequences are placed within, or immediately adjacent to the AT-rich DNA segments framing this domain. Similar correlations exist also within the chicken beta globin gene domain. The positions of these AT-rich and repetitive DNA segments framing the alpha globin gene domain also correlate with other already explored features of long range DNA organisation, as clusters of sites of DNAse I hypersensitivity and differential methylation, sites of Matrix-DNA attachment, and with the beginning and end of the transcribed domain.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00419739
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  • 4
    Electronic Resource
    Electronic Resource
    Conservation and variation in the large scale organisation of the globin gene domains of duck and chicken (1986)
    Springer
    Molecular genetics and genomics 203 (1986), S. 193-201 
    Details
    ISSN: 1617-4623
    Keywords: Duck globin genes ; Cosmid library ; Repetitive sequences ; AT rich sequences
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The genomic DNA of cloned recombinants containing the duck globin genes was compared to that of the analogous domains of the chicken. A 36 kb insert including the three alpha-type globin genes was isolated from a newly prepared duck genomic library in the cosmid PJB8; another recombinant contained a 45 kb insert with the four beta globin genes. In the alpha globin gene domain, the relative positions of genes, of repetitive sequences, and of the A+T-rich segments (AT-rich linkers, ATRLs) which frame the gene cluster (Moreau et al. 1982), were found to be closely maintained between duck and chicken. Although ATRLs and repetitive sequences also frame the gene cluster in the beta globin domains of duck and chicken, there is more genetic drift in their relative positions than in the alpha domain. It is of interest that several repetitive DNA segments were detected in the chicken beta globin domain which do not exist in corresponding positions in the duck. In view of the strict conservation in both species of genes and their relative positions in the cluster, this observation seems to exclude a simple function of repetitive sequences in the control of individual genes. The data are discussed with regard to the possible significance of repetitive and AT-rich DNA segments in genome organisation and function.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00333954
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  • 5
    Electronic Resource
    Electronic Resource
    AT-rich linkers in long range organisation of Drosophila DNA (1985)
    Springer
    Molecular genetics and genomics 199 (1985), S. 357-364 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary AT-rich segments were mapped in 6 different domains of the Drosophila melanogaster genome by partial denaturation of cloned genomic DNA segments and observation in the electron microscope (EM). As found in a previous investigation (Moreau et al., 1982), three types of AT-rich segments could be distinguished: 1) AT-rich linkers with a very high AT content, 600±200 bp long; 2) clusters of such AT-linkers extending over up to 10 Kpb and 3) AT-rich stretches which are shorter and of lower AT content compared to AT-linkers. Six genes previously localized in these domains were found to lie in relatively GC-rich segments framed by AT-rich elements of the 3 types: the Larval Serum Protein LSP-1 α gene is framed by 2 AT-linkers, the LSP-1β and LSP-2 genes by two AT-rich stretches, and the LSP-1γ gene by a cluster and a stretch. The 55 Kbp genomic segment encompassing the P6 gene contains and AT-cluster of about 15 Kbp and several AT-linkers and AT-rich stretches. The 85 Kbp domain containing the P1 gene includes 3 AT-rich clusters of about 10 Kbp each framing GC-rich domains punctuated by AT-linkers and stretches. This study shows that AT-mapping allows a rapid diagnosis of large genomic DNA domains in relation to the AT-rich segments which, possibly, are of significance with regard to genome organisation and function.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00330743
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  • 6
    Electronic Resource
    Electronic Resource
    Transcription of the alpha globin gene domain in normal and AEV-transformed chicken erythroblasts: Mapping of giant globin-specific RNA including embryonic and adult genes (1987)
    Springer
    Molecular genetics and genomics 209 (1987), S. 210-220 
    Details
    ISSN: 1617-4623
    Keywords: Globin genes ; Pre-mRNA ; Transcription ; AT-rich linkers ; Repetitive DNA ; Oncogenes ; AEV
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The genomic domain of about 20 kbp of the chicken alpha-type globin genes, framed by AT-rich linkers (ATRLs; Moreau et al. 1982) and repetitive sequences (Broders et al. 1986), was cut into 13 fragments and subcloned. The in vitro labelled individual restriction fragments were used to test the extent of the transcribed domain by blot-hybridization of nuclear RNA in large excess from normal adult chicken and Avian Erythroblastosis Virus (AEV)-transformed erythroblasts. In both these types of cells, the AT-rich segments situated 6 kbp upstream of the first gene as well as all the domain including the embryonic pi and the adult alpha D and alpha A genes down to the AT-rich segment placed 3 kbp downstream were found to be transcribed. Electrophoresis of nuclear RNA, Northern blotting and hybridization with most of the nick-translated DNA probes revealed in all cases the presence of heterogeneous globin RNA molecules in the 3–13 kb range, as well as some distinct RNA bands. Single-stranded RNA probes of some genomic segments indicated asymmetrical transcription of the minus strand. A 12 kb globin-specific RNA including the pi and alpha A genes but not the intervening alpha D gene was observed in AEV-transformed cells: it includes sequences located far upstream and downstream from the alpha globin genes and might represent a processing product of a full length transcript spanning the whole domain. Reverse transcription by extension of primers placed in the first exon of each of the three globin genes confirmed the presence of continuous transcripts of the domain including the two adult and the embryonic globin genes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00329645
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    Paper (German National Licenses)
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