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  • 1
    Electronic Resource
    Electronic Resource
    Tests for the presence of gametoclonal variation in barley and wheat doubled haploids produced using the Hordeum bulbosum system (1988)
    Springer
    Theoretical and applied genetics 75 (1988), S. 509-513 
    Details
    ISSN: 1432-2242
    Keywords: Gametoclonal variation ; Barley doubled haploids ; Wheat doubled haploids ; Hordeum bulbosum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary To investigate whether the Hordeum bulbosum system of doubled haploid production generates gametoclonal variation, populations of second generation doubled haploid lines were developed from first generation doubled haploid lines of two barley varieties and three wheat genotypes. In barley, no variation between doubled haploids from doubled haploids was detected for a range of quantitative characters, suggesting the absence of any gametoclonal effects. However, the original selfed-seed stocks were shown to contain cryptic allelic variation for some of the characters investigated. In wheat, gametoclonal variation was detected for ear emergence time, plant height and yield, and its components for two out of the three genotypes investigated. The type and range of variation was similar to that reported from studies of somaclonal variation from immature embryos and gametoclonal variation from anther culture. Generally, the effects appeared to reduce the yield performance of individual lines. The difference in response between the two species and the consequences for the use of the doubled haploid system in breeding programmes are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00276758
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  • 2
    Electronic Resource
    Electronic Resource
    Intrachromosomal mapping of crossability genes in wheat (Triticum aestivum) (1985)
    Springer
    Theoretical and applied genetics 70 (1985), S. 309-314 
    Details
    ISSN: 1432-2242
    Keywords: Crossability genes ; Wheat ; Intrachromosomal mapping ; Rye ; Hordeum bulbosum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Intrachromosomal mapping studies were used to locate the positions of the genes Kr1 and Kr2, which control the crossability of wheat with Hordeum bulbosum, on chromosomes 5B and 5A, respectively. The location of Kr1 was established using the telocentric mapping technique and found to be on the long arm of chromosome 5B, distal to the centromere with a mean recombination frequency of 44.8±3.28%. Kr2 was located on the long arm of chromosome 5A by linkage with the major gene markers Vrn1, controlling vernalization requirement, and q, controlling ear morphology. Kr2 is closely linked to Vrn1, with a mean recombination frequency of 4.8±4.66%, and is distal to q with a mean recombination frequency of 38.1±10.60%. The similar locations of Kr1 and Kr2 on homoeologous chromosomes suggest that these two loci are homoeoallelic. Significant correlations between Hordeum bulbosum and rye crossability confirmed that Kr1 and Kr2 control the crossability of wheat with both species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00304917
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  • 3
    Electronic Resource
    Electronic Resource
    Factors affecting haploid production in wheat using the Hordeum bulbosum system. 2. The effect of the timing of pollination (1987)
    Springer
    Euphytica 36 (1987), S. 497-504 
    Details
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Hordeum bulbosum ; crossability ; timing of pollination ; frequency of fertilization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The effect of manipulating the timing of pollination on the fertilization frequency in wheat × tetraploid Hordeum bulbosum hybridizations was investigated as a possible means of overcoming varietal incompatibility in this cross. Pre- and post-anthesis pollinations were made on previously identified highly crossable and poorly crossable wheat genotypes and the stage of development of the individual florets at pollination was expressed in ‘days from anthesis’ units. In both crossable and non-crossable wheat genotypes, a significant negative linear relationship was observed between floret age and fertilization frequency. The magnitude of the response of early pollination was dependent on the number of alleles for non-crossability present at the Kr loci. The non-crossable cultivar Highbury, possessing more than one allele for incompatibility, showed a slight response. The greatest response was shown by the highly crossable cultivar Chinese Spring, possessing alleles for crossability at all Kr loci. The single chromosome substitution line, Chinese Spring (Hope 5B), possessing the most potent allele for non-crossability, Kr 1, showed an intermediate response. The H. bulbosum genotype had no effect on the magnitude of this response, influencing only the mean fertilization frequency.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00041493
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  • 4
    Electronic Resource
    Electronic Resource
    Factors affecting haploid production in wheat using the Hordeum bulbosum system. 1. Genotypic and environmental effects on pollen grain germination, pollen tube growth and the frequency of fertilization (1987)
    Springer
    Euphytica 36 (1987), S. 483-496 
    Details
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Hordeum bulbosum ; pollen grain germination ; pollen tube growth ; frequency of fertilization ; gibberellic acid ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A quantitative examination of pollen grain germination and pollen tube growth within the overy wall was made in selected wheat × tetraploid H. bulbosum hybridizations, to investigate the effect of the wheat and the H. bulbosum genotype on these characters. As expected, variation at the known crossability loci had no effect on pollen grain germination. The frequency of pollen tube penetration of the ovary wall was, however, severely reduced when the dominant alleles were present. Pollen tube penetration was nevertheless observed in 3 of the 110 ovaries examined of the non-crossable cultivar Highbury. The H. bulbosum genotype had a much smaller effect on these characters, but significant differences between the clones were observed in the numbers of pollen tubes initially penetrating the ovary wall. Although two H. bulbosum genotypes showed no significant differences in the number of ovaries with pollen tubes at the base of the ovule, significant differences in the frequency of fertilization were observed. The possible cause of this discrepancy is discussed. The frequency of fertilization in crossable wheat × H. bulbosum hybridizations was improved by the application of gibberellic acid within 10 minutes of pollination, and reduced by an increase in the ambient temperature from 20°C to 26°C. Fertilization following the pollination of non-crossable wheat genotypes was not affected by either of these factors.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00041492
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  • 5
    Electronic Resource
    Electronic Resource
    Factors affecting haploid production in wheat using the Hordeum bulbosum system. 3. Post-fertilization effects on embryo survival (1987)
    Springer
    Euphytica 36 (1987), S. 763-773 
    Details
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Hordeum bulbosum ; frequency of fertilization ; seed set ; seed survival ; gibberellic acid ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary An investigation was made of factors affecting the frequency of development of fertilized ovules following wheat x Hordeum bulbosum hybridizations. Seed survival, a term used here to represent the relationship between seed set and fertilization, was shown to vary between the wheat genotypes tested, but was unrelated to the crossability genes present within the wheat genotype. A high seed survival was obtained in crosses involving the variety TH3929 and the single chromosome substitution line, Chinese Spring (Hope 5B). Chinese Spring showed a poor seed survival. Some influence of the H. bulbosum genotype on seed survival was detected. The two environmental factors studied, namely the post-pollination application of gibberellic acid and the ambient temperature, were both shown to affect seed survival. The application of gibberellic acid immediately after pollination or 1 and 2 days after pollination improved seed survival, although the extent of the response was dependent upon the number and timing of the application(s). A genotype-dependent response to the ambient temperature was observed, TH3929 showing a slight but insignificant reduction and Chinese Spring a significant improvement in seed survival with an increase in temperature from 20°C to 26°C. The physiological processes which may be influenced by GA and the ambient temperature and may thereby affect seed survival are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00051859
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  • 6
    Electronic Resource
    Electronic Resource
    Doubled haploid production in winter wheat and triticale genotypes, using the Hordeum bulbosum system (1986)
    Springer
    Euphytica 35 (1986), S. 1045-1051 
    Details
    ISSN: 1573-5060
    Keywords: Hordeum bulbosum ; Triticum aestivum ; wheat ; triticale ; haploids ; doubled haploids ; crossability ; seed set ; embryo regeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary An attempt was made to produce doubled haploids on 16 winter wheat and six spring and winter triticale genotypes thought to carry genes for interspecific incompatibility. The potential for haploid production was maximized by the use of Hordeum bulbosum genotypes selected for high crossability on crossable wheat genotypes, the use of two post-pollination applications of gibberellic acid and by the pollination of immature florets. A low frequency of seed was set on both the wheat and the triticale genotypes, having mean seed sets of 0.20 per cent and 0.27 per cent respectively. Although the frequency of embryos (‘seed quality’) was high, doubled haploid production was further limited by poor embryo differentiation and regeneration. Haploid plantlets were obtained from the wheat cultivars Moulin and Renard, although successful chromosome doubling and doubled haploid production was achieved in Moulin only.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00028617
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