ISSN:
1365-2230
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Medicine
Notes:
Spindle cells found in malignant fibrous histiocytoma (MFH) were examined using histochemical, immunohistochemical, ultrastructural and tissue culture studies. They were deeply eosinophilic on haematoxylin-eosin staining, strongly PAS-positive and darkly stained with nicotinamide adenine dinucleotide tetrazolium reductase and menadione linked 2-glycerophosphate dehydroge-nase. They also labelled with anti-S-100 and actin antibodies. Fuchsin positive intra-cytoplasmic filaments were recognizable with phosphotungstic acid haematox-ylin stains. Ultrastructurally, the spindle cells had an indented nucleus and elongated cytoplasm containing myofilaments with electron dense bodies. In tissue culture, the spindle cells had plump cytoplasm and were closely apposed to each other within a sheath of fibro-blasts. Using electron microscopy the cultured spindle cells were found to contain numerous glycogen particles, rod-shaped mitochondria, rough endoplasmic reticulum and bundles of myofilaments within the cytoplasm. The spindle cells were identified as ‘myofibroblasts’ and the presence of myofibroblasts in MFH was thus confirmed. The function of myofibroblasts in malignant fibrous histiocytoma is discussed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1365-2230.1988.tb00738.x
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