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  • 1985-1989  (3)
  • 1
    Electronic Resource
    Electronic Resource
    Comparative study on Lewis lung tumour lines with ‘low’ and ‘high’ metastatic capacity III. Glycosaminoglycan synthesis, transport and degradation in cell lines (1989)
    Springer
    Clinical & experimental metastasis 7 (1989), S. 659-669 
    Details
    ISSN: 1573-7276
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The glycosaminoglycans (GAGs) of low (LM) and highly metastatic (HM) cell lines of the Lewis lung tumour (3LL) were compared using [3H]glucosamine labelling techniques. The GAGs isolated from nuclei, cytoplasm, pericellular fractions and medium were analysed by cellulose acetate electrophoresis and by digestion with specific enzymes, and the following conclusions were drawn. 1. Increased cellular uptake and incorporation of [3H]glucosamine into glycoconjugates of the cytoplasm was a typical feature of the highly metastatic cell line after a 48-h labelling. However, there was no elevated radioactivity in glycolipids. 2. Radioactivity of the purified GAGs was two and three times higher in nuclear and cytoplasmic fractions of HM cells than in those of LM cells. There was much less difference between the two cell lines in the pericellular fractions. 3. A definite change from chondroitin sulphate to dermatan sulphate dominancy was recorded in each GAG fraction. Higher heparan sulphate labelling was observed in the cytoplasmic and pericellular GAGs of HM cultures. 4. In the post-labelling period about three times more GAG was present in the extracellular compartment of the HM cultures compared with the LM cultures. 5. In the LM cultures the total GAG-associated radioactivity decreased by 73 per cent in the 48-h chase period whereas in the HM cultures it decreased by only 30 per cent. This indicates a higher rate of GAG degradation in the LM cultures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01753676
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Two human melanoma xenografts with different metastatic capacity and glycosaminoglycan pattern (1989)
    Springer
    Journal of cancer research and clinical oncology 115 (1989), S. 554-557 
    Details
    ISSN: 1432-1335
    Keywords: Human melanoma ; Metastasis ; Glycosaminoglycan ; Xenograft
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Two human melanoma xenografts were compared with respect to their in vivo growth and metastatic potentials as well as glycosaminoglycan patterns. The less differentiated HT 168 tumor showed faster growth at primary sites and a more pronounced capacity for metastasis into the liver. Although chondroitin sulfate was the dominant glycosaminoglycan subtype in both tumors, the more invasive xenograft had a higher heparan sulfate/chondroitin sulfate (HS/CS) ratio. We suggest that tumor progression is influenced by this ratio in this human melanoma system.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00391357
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Flow cytometric measurements and electron microscopy of cell surface glycosaminoglycans using Acridine Orange (1985)
    Springer
    Journal of molecular histology 17 (1985), S. 71-79 
    Details
    ISSN: 1573-6865
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Cell surface glycosaminoglycans (GAGs) were measured, after various treatments, by their binding to Acridine Organge using flow cytometry. Using a critical electrolyte concentration and combining it with specific degradation of individual GAG elements, it was found possible to differentiate between GAG components. The technique was adapted for electron microscopy level to reveal characteristics of membrane-associated GAG. By this means, the cell membrane of the human leukaemic cell line K562 was shown to contain a large amount of GAG; 75% of it was highly sulphated GAG, mostly heparan sulphate. This component was evenly distributed in the outer plasma membrane layer. In the presence of other GAGs, the appearance of complex proteoglycan granules was detected.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01003404
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    Paper (German National Licenses)
    Fulltext
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