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  • 1
    ISSN: 1432-1076
    Keywords: Protein phosphorylation ; Neutrophil ; Chronic granulomatous disease
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The phosphorylation and dephosphorylation of proteins in neutrophils from normal children and patients with chronic granulomatous disease (CGD) were studied with two-dimensional gel electrophoresis and autoradiography, followed by densitometric scanning. In normal neutrophils the radio-activities of 11 spots among approximately 50 radioactive spots were changed by stimulation with phorbol 12-myristate 13-acetate (PMA) and 6 of the 11 spots were also changed by stimulation with N-formyl-methionyl-leucyl-phenylalanine (FMLP) and NaF. The phosphorylation of only two spots (Mr=48000 and 62000) was inhibited by 2-deoxyglucose and N-(6-aminohexyl)-5-chloro-1-naphthalene sulphonamide (W-7), which inhibits superoxide production, while it was not affected by dibutyryl cAMP, KCN and ethyleneglycol-bis-(β-aminoethylether)-N,N′-tetraacetic acid (EGTA), which do not affect superoxide production. The observation indicates that the Mr=48000 and 62000 proteins may be involved in the activation process of superoxide production. When the neutrophils of four male and two female CGD patients were examined, the changes in 11 spots on stimulation were similar to those of normal children, indicating that the (de)phosphorylation of the proteins which seems to be involved in the activation process is not affected in CGD neutrophils.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-1076
    Keywords: Primary hypothyroidism ; Pituitary enlargement ; Slipped capital femoral epiphysis ; Cystic ovaries
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract A case of primary hypothyroidism with pituitary enlargement, slipped capital femoral epiphysis and cystic ovaries is reported. The pituitary abnormality and cystic ovaries disappeared dramatically after thyroid hormone therapy. Hip pinning was performed. The recognition of these associations may eliminate unnecessary surgery and lead to the choice of hormone replacement therapy.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 66 (1989), S. 5851-5853 
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: Using a new process, a large grain polycrystalline silicon layer was grown directly from molten Si on a single-crystal silicon wafer at a rate 100 times faster than the conventional chemical vapor deposition process. A polycrystalline silicon layer was also produced on a single-crystal silicon wafer, which had an oxide layer and etched V grooves, through use of an additional ultrasonic wave effect in this process. A layer thickness of 100–600 μm and a grain size of 10 μm–3 mm were controllably obtained. After the growth of the polycrystalline layer, dislocation densities of 4×105 cm−2 for the single-crystal silicon substrate, and 2×106 cm−2 for the polycrystalline layer were obtained. Some devices were fabricated from the products of this new process, and the characteristics of these devices were investigated.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    [S.l.] : American Institute of Physics (AIP)
    Review of Scientific Instruments 56 (1985), S. 1036-1037 
    ISSN: 1089-7623
    Source: AIP Digital Archive
    Topics: Physics , Electrical Engineering, Measurement and Control Technology
    Notes: A method combining the techniques of laser blowoff and laser-induced fluorescence has been developed in order to measure the local electron density below 1013 cm−3. Characteristics of a Li0 beam produced by laser blowoff of a thin Li film are investigated using beam–plasma interactions and laser-induced fluorescence. Such a beam has a near-Maxwellian velocity distribution with a temperature around 4.5 eV and a density of the order of 1010 cm−3 at a distance of about 1 m from the film target. The feasibility of measuring electron density with this Li0 beam and a dye laser is demonstrated with an ECR plasma.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1420-9071
    Keywords: Gastrin-like immunoreactivity ; CCK8/gastrin-like immunoreactivity ; antrum ; pituitary ; rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Gel filtration of antral extract of adult rats revealed gastrin-17 and gastrin-34. Gel filtration of anterior pituitary extract showed CCK8 and gastrin-17, whereas posterior pituitary extract showed only a CCK8. Antral gastrin-like immunoreactivity (G-LI) increased after milk feeding and especially after weaning was started. Changes in diet may exert a profound influence on the ontogenic development of antral G-LI, but not pituitary CCK8/G-LI.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1573-2665
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We have described the clinical and biochemical status of two patients with tetrahydrobiopterin (BH4) deficiency due to impaired dihydrobiopterin biosynthesis. BH4 administration appeared to improve the mental and psychological status more than did neurotransmitter replacement therapy alone. This enhancement of activities of daily life was seen with a dose of BH4 as low as 1.25 mg kg−1 day−1. Granulocyte adherence capacity was below normal and recovered after BH4 therapy in both patients. B-cell differentiation capacity was altered either before or after therapy.
    Type of Medium: Electronic Resource
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