Electronic Resource
Oxford, UK
:
Blackwell Publishing Ltd
Journal of oral pathology & medicine
13 (1984), S. 0
ISSN:
1600-0714
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Medicine
Notes:
To study the regulation of collagen degradation in periodontium, human gingival homogenate was incubated at 36°C and the release of hydroxyproline was assayed as a measure of collagenase activity. Phenylmethylsulfonyl fluoride, soybean trypsin inhibitor and scrum albumin inhibited the in vitro collagenolysis while p-aminophenylmcreuric acetate, a sulfhydryl reagent, increased the degradation. When latent collagenase obtained from gingival fibroblast culture was added to the incubation a marked increase in the collagen degradation was found. This increase was prevented by phenylmethylsulfonyl fluoride. The data suggests that collagenase may exist in gingiva partly in a latent form and its activation may be brought about by 2 mechanisms. A serine proteinase present in tissue may activate collagenase by producing a limited clevage, or the activation may occur through a reaction that involves the sulfhydryl groups of the collagenase molecule.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1600-0714.1984.tb01441.x
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