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  • 1980-1984  (9)
  • 1965-1969  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Orotidine 5'-monophosphate decarboxylase inhibitors formed by spontaneous reaction of barbituric acid and ribose 5-phosphate, a surprising reaction (1980)
    s.l. : American Chemical Society
    Journal of the American Chemical Society 102 (1980), S. 1208-1210 
    Details
    ISSN: 1520-5126
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/ja00523a072
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Additions and Corrections - Bacteriorhodopsins Containing Cyanine Dye Chromophores. Support for the External Point-Charge Model (1983)
    s.l. : American Chemical Society
    Journal of the American Chemical Society 105 (1983), S. 4501-4501 
    Details
    ISSN: 1520-5126
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/ja00351a605
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Computer evaluation of sprinkler irrigation uniformity (1980)
    Springer
    Irrigation science 2 (1980), S. 23-32 
    Details
    ISSN: 1432-1319
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A method for evaluating the water application rate (WAR) and uniformity coefficient (Cu) of overlapping irrigation sprinklers is given for realistic field conditions which includes wind drift of the sprinkler spray. The method requires as input — the geometry of the sprinkler arrangement, trajectories of water drops from the sprinkler nozzle as calculated by the equations of motion and the WAR distribution (discharge) profile of a single sprinkler experimentally observed under windless conditions. Wind direction with respect to the main sprinkler line is shown to have a small effect on Cu and is assumed to be parallel to the main line. Results show that the effect of wind drift of sprinkler spray on Cu can be neglected for wind velocities less than 1 ms−1 (Fig. 8). Analysis of simulated discharge profiles (Table 1) shows that the maximum value of the uniformity coefficient was obtained with triangular sprinkler discharge profiles at low values of spacing, changing to trapezoidal profiles as the spacing increases (Figs. 8 and 9). The effect of nozzle pressure on WAR was evaluated for the pressure range between 294 and 490 kPa and an optimum layout of overlapping sprinklers, designed to minimize the effect of wind drift and nozzle pressure on the uniformity of WAR distribution, is presented.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00285427
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Cytoflurometric nuclear DNA-determinations in infant, adolescent, adult and aging human hearts (1983)
    Springer
    Histochemistry and cell biology 77 (1983), S. 485-494 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The progress of polyploidization in the human heart muscle cell was investigated by cytofluorometry, involving selective measurements of heart muscle cell nuclei. Thirty-two tissue samples, taken from the free wall of the left ventricle of each autopsied heart, were fixed in Carnoy's fluid. From thick (100–150 μm) paraffin sections, isolated cells for smears were obtained by enzyme digestion and ultrasonic treatment. The smears were stained with azocarmin G to eliminate background fluorescence and subsequently stained by an acriflavine-Feulgen reaction. Cytofluorometric DNA-determinations were carried out selectively on heart muscle cell nuclei, using the muscle striations revealed by azocarmin G-fluorescence as specific markers. The dynamic process of polyploidization in normal hearts could be divided into four stages. In the first stage (under 1 year of age), almost all heart muscle cell nuclei (94.3±1.8%) were diploid. In the second stage (1 to 9 years of age), the number of tetraploid nuclei increased (13.6±7.1%). In the third stage (9 to 22 years of age), octaploid nuclei first appeared and the number of tetraploid nuclei increased (26.7±3.9%). The DNA pattern in the fourth stage (22 to 75 years of age) was relatively constant, with a ratio of diploid (62.4±8.7%), tetraploid (31.4±6.7%) and octaploid (5.8±3.9%) nuclei. From these results it was concluded that physiological polyploidization progresses in proportion to the increase of heart weight. The frequency of polyploid nuclei in human heart was not so high as resported by previous investigators.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00495803
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Fluorescence fading and stabilization in cytofluorometry (1980)
    Springer
    Histochemistry and cell biology 65 (1980), S. 269-276 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The factors affecting fluorescence fading in cytofluorometry were investigated using different kinds of nuclear staining, mounting media, and procedure of specimen preparation. Acceleration of fluorescence fading was observed in smear specimens treated with RNase, trypsin, or hypotonic solution before pararosaniline Feulgen nuclear staining. Similar effect was found for other DNA-stainings such as “33258 Hoechst” and Feulgen reactions with different Schiff-type dyes, such as acriflavine-SO2 and cresylviolet-SO2, when chemically pure DNA was used. Fluorescence decay was rapid for all fluorochromes examined, when glycerin or buffer solution was used as mounting medium. Marked stabilization of fluorescence emission was induced in specimen mounted in non-fluorescent resin, Entellan (Merck), after post-staining fixation with absolute methanol for all tested fluorochromes. The same treatment induced almost complete fluorescence stabilization of fluorescein isothiocyanate (FITC); no detectable fluorescence fading was observed in a specimen stained with indirect immunofluorescence reaction using anti-UV-DNA antibody, during storage for 2 years at room temperature without special protection against light. These observations suggest that factors which bring about conformational stability of macromoleculedye complexes generally induce fluorescence stabilization.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00493176
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    Cytofluorometry on cells isolated from paraffin sections after blocking of the background fluorescence by azocarmin G (1981)
    Springer
    Histochemistry and cell biology 71 (1981), S. 161-170 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A technique for isolation of cells from paraffin embedded tissue is indispensable for the performance of Feulgen-DNA cytofluorometry in parallel with the definition of histological characteristics. Background fluorescence due to nonspecific dye-binding by a “pseudo-plasmal reaction” is usually found to be so intense on cells isolated from formalin-fixed tissues, that we are often forced to abandon quantitative DNA determinations. In the present work, we report that fixation of tissues with Carnoy's fixative for 12 h at 5° C not only reduces nonspecific dye-binding but also facilitates the process of cell isolation. Furthermore, we find that pre-treatment of cells isolated from Carnoy-fixed tissues with acidic azocarmin G solution completely blocks nonspecific dye-binding in subsequent acriflavine Feulgen nuclear staining. This combination of techniques for specimen preparation enables us to carry out Feulgen-DNA cytofluorometry on cells isolated from histological sections with satisfactorily low coefficients of variation (less than 8%). The techniques should be widely applicable for parallel DNA determinations and histology.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00507820
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  • 7
    Electronic Resource
    Electronic Resource
    Nonspecific (“pseudo-plasmal”) dye-binding in the Feulgen nuclear stain and its blocking by azocarmin G (1980)
    Springer
    Histochemistry and cell biology 66 (1980), S. 169-180 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In Feulgen nuclear staining nonspecific dye-binding due to the “pseudo-plasmal reaction” is intensified in isolated cells with intact cytoplasm, and cannot be eliminated by the post-irradiation method. Fluorescence intensity in the cytoplasm sometimes exceeds that of specific nuclear fluorescence, especially in brain and heart muscle cells, and it was almost impossible to perform cytofluorometric DNA quantification on such specimens. Various kinds of aldehyde-blocking agents such as sodium borohydride, 2,4-dinitrophenylhydrazine, aniline, and sodium pyrosulfite were effective in reducing the “pseudo-plasmal reaction”. But the blocking effects were not complete because of additional release of reactive aldehyde groups during subsequent Feulgen hydrolysis. Acidic azocarmin G produces a complete block of all “pseudo-plasmal reaction” in acriflavine-Feulgen nuclear staining, allowing accurate DNA-cytofluorometry to be carried out.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00494643
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    Paper (German National Licenses)
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  • 8
    Electronic Resource
    Electronic Resource
    Detection of laser-UV microirradiation-induced DNA photolesions by immunofluorescent staining (1980)
    Springer
    Human genetics 〈Berlin〉 54 (1980), S. 107-110 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A low-power laser-UV microbeam of wave-length 257 nm was used for microirradiation of a small part of the nucleus of Chinese hamster cells. Following fixation in interphase or in the subsequent metaphase indirect immunofluorescent staining was performed with antiserum to photoproducts of DNA treated with far UV light. The results show that antibodies specific for UV-irradiated DNA can be used for a direct detection of laser-UV microirradiation-induced DNA photolesions. The potential usefulness of this method for investigation of the spatial arrangement of chromosomes in the interphase nucleus is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00279058
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    Paper (German National Licenses)
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  • 9
    Electronic Resource
    Electronic Resource
    Pulse response in an immobilized-enzyme column: Theoretical method for predicting elution curves (1980)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 22 (1980), S. 779-797 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A theoretical method for predicting the elution profile of a pulse response in an immobilized-enzyme column is proposed. The method is based on a mass balance model, which is extensively used in gel chromatography. To test the method, a pulse of sucrose solution was applied to a column of spherical acrylamide gel in which was entrapped invertase from yeast, and it was eluted with 0.05M acetate buffer at pH 5.0. The elution curves of the substrate and the product were in fairly good agreement with the theoretically calculated ones. The method was extended to the system of reversible as well consecutive reactions.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260220405
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    Paper (German National Licenses)
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  • 10
    Electronic Resource
    Electronic Resource
    Mass spectrometric studies of cycloalkane-α-glycols (1968)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 1 (1968), S. 61-70 
    Details
    ISSN: 0030-493X
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The mass spectral fragmentations of stereoisomeric cyclohexane-1,2-diols, p-menthane-2,3-diols, 1-hydroxycarvomenthols and 4-hydroxymenthols are discussed.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/oms.1210010107
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