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Potentiometric quantitation of glycerol using immobilized glycerol dehydrogenase (1982)

Chen, A. K. ; Starzmann, J. A. ; Liu, C. C.

New York, NY [u.a.] : Wiley-Blackwell

Biotechnology and Bioengineering 24 (1982), S. 971-975

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Details

ISSN: 0006-3592

Keywords: Chemistry ; Biochemistry and Biotechnology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology

Notes: Glycerol dehydrogenase was immobilized in polyacrylamide gel layered over a small platinum screen and used to catalyze the oxidation of glycerol. In the presence of NAD+ and potassium ferricyanide, the coupling reaction generated a measurable electrical potential which was found to be Nernstian with respect to the glycerol concentration range of 10-4M to 10-1M. The reproducibility of the measurement and the optimal conditions for glycerol determination were described.

Additional Material: 1 Ill.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/bit.260240416

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