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Digitale Medien

Miniaturization of a standard liquid chromatographic system (1980)

Hermansson, J.

Springer

Chromatographia 13 (1980), S. 741-744

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Details

ISSN: 1612-1112

Schlagwort(e): Miniaturization ; HPLC ; Micro-columns ; 0.67 mm3 micro flow-cell ; Sulphonamides

Quelle: Springer Online Journal Archives 1860-2000

Thema: Chemie und Pharmazie

Notizen: Summary Methods for the construction of micro-columns equipped with low dead volume end fittings and an easy way to miniaturize a standard flow-cell to a volume of 0.67 mm3 are described. Operation characteristics of the microcolumns were evaluated by plate height versus linear velocity and plate height versus capacity factor plots. The influence of the volume of the flow-cell on the resolution is also demonstrated. An improvement of the resolution factor (RS) from 0.9 to 1.45 between sulphapyridine and sulphamerazine was obtained by using the 0.67 mm3 flow-cell instead of the 12.5 mm3 original flow-cell. The influence of the sample volume on the separation efficiency is also shown.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF02265552

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