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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Diabetologia 25 (1983), S. 291-295 
    ISSN: 1432-0428
    Keywords: Mammalian embryo culture ; diabetes ; glucose ; hyperglycaemia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary To elucidate the role of hyperglycaemia in causing fetal malformation, rat embryos have been grown in vitro from the head-fold stage for 48 h in the presence of excess glucose during different parts of the culture period. Culture of rat embryos for 10 or 21 h in 55 mmol/l exogenous glucose produced abnormalities observable after 48 h in culture. When embryos cultured for 10 or 21 h in excess glucose were observed by scanning electron microscopy at the end of the glucose treatment, abnormalities could be observed which may indicate how later malformations are formed. Thus it is possible that a relatively brief hyperglycaemic episode at a critical stage of embryogenesis may endanger the fetus of a diabetic mother.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-2013
    Keywords: Electron microprobe analysis ; Sympathetic neurones ; Cellular electrolyte concentrations ; Carbachol ; Ouabain
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Intracellular element concentrations were measured in rat sympathetic neurones using energy dispersive electron microprobe analysis. The resting intracellular concentrations of sodium potassium and chloride measured in ganglia maintained for about 90 min in vitro at 25° C were 3, 155 and 25 mmol/kg total tissue wet weight respectively. Recalculated in mmol/l cell water, these values are 5, 196 and 32 respectively. There were no significant differences between the nuclear and cytoplasmic values of these ions. Incubation in either carbachol (108 μmol/l, 4 min) or ouabain (1 mmol/l, 60 min) significantly increased the intracellular sodium and decreased the intracellular potassium concentrations. Neither substance materially altered the intracellular chloride concentration. The data obtained are compared and contrasted to those obtained in mammalian sympathetic neurones using chemical analysis and ion-sensitive microelectrodes.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Pflügers Archiv 399 (1983), S. 241-242 
    ISSN: 1432-2013
    Keywords: Single renal tubules ; Electron microprobe analysis ; Cellular concentrations
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract A method is described whereby single renal tubules, incubated in varying incubation media can be captured and prepared for electron microprobe analysis for intracellular sodium, potassium, chloride, phosphorus, magnesium amongst others.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    The European physical journal 299 (1981), S. 85-89 
    ISSN: 1434-601X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract High-spin levels in189Hg have been populated via the175Lu(19F, 5n) reaction. A rotation-aligned band built on a 13/2+ state originating from theνi 13/2 subshell is identified which is interpreted within the framework of “quasiparticle+rotor” models. A negative parity band built on a 21/2− state which can be accounted for by a three quasi-particle state is also observed.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1434-601X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract High-Spin states in188Hg have been investigated using the168Er(24Mg,4n) reaction. The rotation aligned vi 13 2/2 band, which intersects the weakly oblate ground state band atI≈10, is observed up toI π =20+ with an isomeric 12+ level with T1/2=134(15)ns. The coexisting strongly deformed band, built on the 825 keV 0+ level, is observed up to the 14+ state. The systematics of the vi 13 2/2 rotation aligned bands is discussed.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    The European physical journal 313 (1983), S. 289-292 
    ISSN: 1434-601X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Using the reaction175Lu(19F, 6n) theg-factor of the188Hg isomeric state (J π=12+,T 1/2=135 ns) has been measured with the TDPAD method. The experimental value g=−0.168(10) supports the interpretation of an almost pure (vi13/2)−2 configuration for this isomer.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Journal of applied electrochemistry 12 (1982), S. 505-515 
    ISSN: 1572-8838
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Electrical Engineering, Measurement and Control Technology
    Notes: Abstract Natural graphite flakes (80 wt%), with polypropylene (20 wt%) as a binder, constitute a practical and non-expensive graphite electrode of high crystallinity ‘CPP’. Galvanostatic cycling of these electrodes with current densities in the range 0.3–30 mA cm−2 (charging time 5–120 min) has been investigated in aqueous acids (12, 20 and 36 mol dm−3 HF, 6 and 12 mol dm−3 H2SO4, 4 mol dm−3 HClO4). The anion of the acid is anodically intercalated and cathodically de-intercalated. In spite of the high water concentrations, quantitative current efficiencies have been obtained. From variation of the rest times after charging, a corrosion current density of less than 0.03 mA cm−2 (j ch=3 mA cm−2) has been derived. The overvoltage during charge and discharge is typically about 0.1 V. The potential at the start of the charging process coincides with the intercalation potential defined previously. A strong electrode formation effect is observed upon cycling. The electrode is initially smooth and non-porous; it acquires a high surface roughness after a few cycles, which is then stable. The initial charging curves increase witht 1/2, while the charging curve after electrode formation is linear. Both clearly indicate a linear relationship between surface concentration of intercalated anions and potential. This agrees with our previous finding of linear dependence with respect to acid concentration in the solution.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1434-601X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract High spin states in the nucleus154Er have been reinvestigated using the123Sb(35Cl, 4n) reaction and a variety of spectroscopic techniques including excitation functions,γ-γ coincidences,γ angular distribution and linear polarization measurements. From the measured energies, relative intensities and transition multipolarities a new level scheme has been deduced up to an excitation energy of ∼12 MeV and spin 36. An interpretation of the experimental results is given in terms of the deformed Woods-Saxon orbitals. Gigantic backbending (superdeformation) effect is studied theoretically within the cranking model.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Journal of molecular histology 13 (1981), S. 667-679 
    ISSN: 1573-6865
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Enbryopathy has been produced by inhibition of histiotrophic nutrition in the rat using a number of agents which prevent this process. The experiments were carried outin vitro at various stages of development before the inception of a chorio allantoic placenta. Dose-dependent effects on the embryo were demonstrated using the acid bisazo dye Trypan Blue, which inhibits endocytosis, and an enzyme inhibitor of bacterial origin known as leupeptin which inhibits cathepsin B, H and L. Homogenates of rat kidney and placenta also produced congenital defects; the concommitant electron microscopical changes in the yolk sac suggest that these effects too are due to alterations in the availability or quality of histiotroph.
    Type of Medium: Electronic Resource
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