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  • 1980-1984  (6)
Source
Material
Years
Year
  • 1
    Electronic Resource
    Electronic Resource
    Simple and rapid separation of ortho- and paramyxovirus glycoproteins (1980)
    Springer
    Medical microbiology and immunology 168 (1980), S. 249-259 
    Details
    ISSN: 1432-1831
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The hemagglutinin (HA) and neuraminidase (NA) of influenza viruses, as well as the fusion protein (F) and hemagglutinin-neuraminidase (HN) of paramyxoviruses, have been separated in native form using a two-step procedure. The glycoproteins are efficiently extracted from virions using the non-ionic detergent octyl-Β-D-glucoside and are then applied to a column of agarose beads coupled with tyrosine-sulfanilic acid. PureHA andF are obtained in good yield in the flow-through from this column.NA andHN bind strongly and can be eluted, albeit somewhat contaminated withHA orF, by raising the pH of the column buffer. The separated non-denaturated fractions can be used for structural, functional, and antigenic studies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02121808
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Evidence for the contribution of the host species to the extent of antigenic variation of N1 influenza virus neuraminidase (1983)
    Springer
    Medical microbiology and immunology 172 (1983), S. 1-11 
    Details
    ISSN: 1432-1831
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract N1 influenza virus neuraminidases (NA) derived from avian, swine and human virus isolates, including the genetically related classic strains A/FPV/Rostock/34, A/Swine/1976/31, A/PR8/34 and A/FM1/47, were analysed serologically by neuraminidase inhibition (NI), inhibition of virus release (IVR) and competitive radio-immunoassays (competitive RIA). Comparing the three tests, competitive RIA appeared to be more reliable than NI and IVR for a quantitative assessment of antigenic relatedness. Together with evidence presented by others, these studies indicate that the host species contributes to the extent of antigenic variation of NAses. In contrast to NAses of human viruses where antigenic drift occurs readily, NAses of animal influenza viruses, from birds or mammalians, undergo far fewer antigen changes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02123672
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Viral glycoproteins as determinants of pathogenicity (1982)
    Springer
    Medical microbiology and immunology 170 (1982), S. 145-153 
    Details
    ISSN: 1432-1831
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Conclusion With myxoviruses we are now beginning to understand the complex biological phenomenon of pathogenicity at the molecular level. Proteolytic activation of the viral glycoproteins proved to be a very important determinant for pathogenicity. Variations in pathogenicity are the result of structural variations of the glycoproteins. The available evidence indicates that these structural variations are confined to the cleavage site, i.e., to a small but functionally important part of the molecule.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02298195
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    The quasimolecular 1sσ orbital in collision systemsα(Z 1+Z 2)≳1, its excitation and spectroscopy (1980)
    Springer
    The European physical journal 296 (1980), S. 11-21 
    Details
    ISSN: 1434-601X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract An analytical formula for the excitation probability of the quasimolecular 1sσ orbital as a function of the impact parameterb is derived for collision systems withα(Z 1+Z 2)≳1. This formula describes well all existing experimental data for those collision systems except for the heaviest system Pb+Cm (Z 1+Z 2=178) at impact parametersb≲40 fm. It is discussed in which way energies of the quasimolecular 1sσ orbital can be extracted from experimental 1sσ vacancy production data.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01415610
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Study of high energetic electrons emitted during heavy ion collisions (1982)
    Springer
    The European physical journal 304 (1982), S. 207-219 
    Details
    ISSN: 1434-601X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Double differential cross sectionsd 2 σ/dΩdE were measured for high energetic electrons emitted in heavy ion collisions. Electrons were detected in the energy range of 60 keV-500 keV for various target projectile combinations 66≦Z u =Z t +Z p ≦145 and projectile velocities between 7 % and 10 % of the speed of light. Clear evidence was found that these electrons stem from the united atom formed during the collision. Slope and height of the spectra are discussed with respect to the momentum distribution of strongly bound states (i.e. theL-shell) at momenta far above the mean value. In addition for the systems S, Ni, Br→Pb electrons were detected in coincidence withK x-rays of Pb. By this method the contribution of theK-shell of the combined system to the total spectrum could be separated. Binding energies of theK-shell were estimated by a slope comparison between the coincident and single spectrum. The resulting values are close to the united atom limit.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01414495
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    The impact parameter dependence of the K MO X-ray emission in208Pb +208Pb collisions (1984)
    Springer
    The European physical journal 319 (1984), S. 239-251 
    Details
    ISSN: 1434-601X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The emission probabilities for quasimolecular K-x-radiation (K-MOR) have been measured in208Pb +208Pb collisions at 4.3 MeV/u and 4.8 MeV/u as a function of the scattering angle using the particle-photon-coincidence technique. The probabilities exhibit a superposition of two exponential slopes with different fall-off constants. We identify the sharp fall off at very small impact parameters (〈60 fm) with the K-MOR contribution from the decay of 1sσ-vacancies. The flatter slope for impact parameters larger than 60 fm is attributed to the radiative decay of holes in 2p 1/2 σ molecular states. In this way, experimental “1sσ-MOR-emission probabilities” could be extracted and compared with theory. For 4.3 MeV/u the1sσ- and 2p 1/2 σ-excitation probabilities could also be determined and have been compared to coupled-channel calculations and to a scaling law for 1sσ excitation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01412536
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    Paper (German National Licenses)
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