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  • 1980-1984  (4)
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  • 1
    Digitale Medien
    Digitale Medien
    In Vitro Cultivation of Bloodstream Forms of Trypanosoma brucei, T. rhodesiense, and T. gambiense (1981)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 28 (1981), S. 0 
    Details
    ISSN: 1550-7408
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Notizen: . A series of new in vitro systems for the cultivation of bloodstream forms of Trypanosoma (Trypanozoon) brucei brucei, T. (T.) b. rhodesiense, and T. (T.) b. gambiense was developed. The standard system consists of a feeder layer of fibroblast-like cells derived from embryos of New Zealand White rabbits (REF) or a mountain vole, Microtus montanus (MEF), with HEPES-buffered Minimum Essential Medium (MEM), with Earle's salts, supplemented with 15% inactivated rabbit serum. These two and other feeder layers were cross-checked with different sera to test for growth support of bloodstream forms of the three trypanosome subspecies studied. Cultures could be initiated with bloodstream forms from mammalian hosts or from cryopreserved stabilates. Metacyclic forms from infected Glossina m. morsitans could also be used as inoculum; they transformed within 6 h to bloodstream forms. Maintenance of cultures and growth properties are described in detail. Experiments were undertaken to confirm that the cultivated bloodstream forms still possess some of the characteristic features of pleomorphic bloodstream populations. Cultivated bloodstream forms were always infective for mice, and a surface coat could be demonstrated by electron microscopy. They could also be cyclically transmitted through tsetse flies, and the metacyclic forms from these flies could be brought back into culture. In vitro cloning with single bloodstream forms and metacyclic forms could be achieved with high cloning efficiency. The consumption of glucose and the production of pyruvate and lactate were determined.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1550-7408.1981.tb05322.x
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  • 2
    Digitale Medien
    Digitale Medien
    Cyclical transmission of in vitro cultivated bloodstream forms and procyclic trypomastigotes ofTrypanosoma brucei brucei byGlossina morsitans morsitans (1982)
    Springer
    Parasitology research 68 (1982), S. 1-5 
    Details
    ISSN: 1432-1955
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Abstract In vitro cultivated bloodstream and procyclic forms ofTrypanosoma b. brucei STIB 247 were cyclically transmitted byGlossina m. morsitans. The tsetse flies were infected artificially on a silicon membrane. Metacyclic trypanosomes from mature salivary gland infections were used to initiate bloodstream form cultures. They transformed into slender bloodstream forms and gave rise to established cultures that proved to be infective for the vector. The metacyclic forms retained the strain-specific basic set of variable antigen types.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00926651
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  • 3
    Digitale Medien
    Digitale Medien
    Stimulating effect of citrate and cis-aconitate on the transformation ofTrypanosoma brucei bloodstream forms to procyclic forms in vitro (1981)
    Springer
    Parasitology research 66 (1981), S. 17-24 
    Details
    ISSN: 1432-1955
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Abstract Transformation ofTrypanosoma brucei bloodstream forms to procyclic forms can be stimulated in vitro by adding 2–10 mM citrate and/or cis-aconitate to the culture medium for at least 3 h. Optimal transformation was obtained in the presence of 3 mM cis-aconitate for 48 h. Citrate and cis-aconitate may activate isocitrate dehydrogenase and/or other Krebs' cycle enzymes and thus act as a metabolic trigger for the morphological changes involved in transformation.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00941941
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  • 4
    Digitale Medien
    Digitale Medien
    Cultivation of procyclic trypomastigotes ofTrypanosoma congolense in a semi-defined medium with direct adaptation from bloodstream forms (1982)
    Springer
    Parasitology research 67 (1982), S. 129-135 
    Details
    ISSN: 1432-1955
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Abstract Bloodstream forms ofTrypanosoma congolense added to the semidefined medium 109-c (Minimum Essential Medium with Earle's salts, 60 mM HEPES, haemin, and heat-inactivated FBS) and incubated at 28° C transformed into procyclic trypomastigotes. Established cultures of the procyclic forms yielded up to 4.5×107 parasites/ml. The cultivated populations consisted only of trypomastigotes and were not infective for mice. The amino acids consumed to the greatest extent were glutamine, proline, and threonine. Changes in glucose, pyruvate, and lactate concentrations during growth were determined. The procyclic organisms possessed a well-developed mitochondrion.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00928108
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