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  • 1980-1984  (7)
Source
Material
Years
Year
  • 1
    Electronic Resource
    Electronic Resource
    Rheumatoid nodules (1984)
    Springer
    Histochemistry and cell biology 80 (1984), S. 463-467 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The presence and localization of fibrin and fibronectin in rheumatoid nodules were studied using an indirect immunoperoxidase technique on tissue specimens fixed in formaldehyde, embedded in paraffin and pretreated with pepsin and testicular hyaluronidase. Three zones characteristic for rheumatoid nodules was recognized. 1) Central area with necrosis, containing at least in part fibrinogen-antigenic material and fibronectin especially in the peripheral part of the necrotic area. 2) Around the necrosis a layer of mesenchymal cells in a palisade arrangement was found. Especially in the external part of this layer fibronectin was demonstrated around and between the cells, where fibrin was absent. 3) Peripherally, a zone of non-specific granulation tissue containing moderate amount of fibronectin decreasing towards the surround mature connective tissue, was seen. In the border of the cellular layer vessels were found in variable amount. In some of the vessels vasculitis was demonstrated with the presence of inflammatory cell infiltration, fibrin deposition and occasionally thrombosis. The pathogenesis of the inflammatory reaction in rheumatoid nodules is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00495435
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Sequential appearance of fibronectin and collagen fibres in experimental arthritis in rabbits (1984)
    Springer
    Histochemistry and cell biology 80 (1984), S. 39-44 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The sequential changes in the presence of fibronectin in the synovial membrane during the development of antigen-induced arthritis in rabbits were studied using an indirect immunoperoxidase technique on the tissue specimens fixed in formaldehyde, embedded in paraffin and pretreated with pepsin and testicular hyaluronidase. The relation to the distribution of fibronectin and connective tissue fibres, demonstrated as either argyrophilic or red by van Gieson method, was studied. Initial after the induction of the arthritis the synoviocytes became increased in size and number. The subsynoviocytial tissue was invaded by granulocytes and the number of vessels was increased. Fibronectin in increased amount was seen around the lining cells. After 2–4 weeks a markedly reduced amount of granulocytes were seen together with an increase in the number of macrophages. At this stage, fibronectin was also found together with argyrophilic fibres in the subsynoviocytial connective tissue. After 8–13 weeks the synovial membrane was found hypertrophic and folded. The lining layer was unchanged, but in the subsynoviocytial tissue lymphocytes and plasma cells were more focally arranged. At that time fine fibres, stained by the van Gieson method, were present together with fibronectin and argyrophilic fibres in the subsynoviocytial tissue. The morphological change and the distribution of fibronectin in experimentally induced arthritis correlates temporally to the morphological change and the presence of fibronectin found in experimentally induced granulation tissue.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00492769
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Demonstration of fibronectin in human articular cartilage by an indirect immunoperoxidase technique (1982)
    Springer
    Histochemistry and cell biology 76 (1982), S. 51-56 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Fresh frozen tissue sections of human articular cartilage was treated without and with human testicular hyaluronidase (2×106 units/l) for 60 min at 37° C and stained by the indirect immunoperoxidase technique with rabbit antihuman fibronectin. The rabbit antihuman fibronectin was purified by affinity chromatography on human fibronectin-Sepharose. Fibronectin was only found on the acellular surface of the articular cartilage in tissue sections not treated with hyaluronidase. In this surface layer, probably identical to “lamina splendens”, the arrangement of fibronectin was as a membrane. No collagen was seen in this area by van Gieson staining. No staining for fibronectin was found in the cartilage matrix or in the chondrocytes. Treatment of the cartilage tissue with hyaluronidase resulted in visualization of high amount of fibronectin in the cartilage matrix, with the highest intensity around the chondrocytes. The staining of the acellular surface layer of the articular cartilage was identical with the results obtained without hyaluronidase treatment. These results indicate that articular cartilage is rich in fibronectin probably in complex with hyaluronic acid, and that the chondrocytes produce fibronectin in situ. It also demonstrates the steric hindrance of hyaluronic acid aggregates in diffusion of the antibody and the value of hyaluronidase treatment of tissue before demonstration of fibronectin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00493284
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    The influence of fixation and tissue preparation on the immunohistochemical demonstration of fibronectin in human tissue (1981)
    Springer
    Histochemistry and cell biology 72 (1981), S. 291-299 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The influence of fixation and tissue preparation on the immunohistochemical localization of human fibronectin in gastrointestinal tract tissue has been examined using indirect immunoperoxidase technique. The most optimal staining result with strong intensity and well defined localization was obtained on frozen sections of unfixed material. Nearly identical results with improved morphology were obtained when staining paraffin sections of tissue fixed in 96% ethanol, 96%+1% acetic acid and absolute acetone. All other fixatives tested, 10% neutral buffered formalin, Lillie's AAF, Bouin's fixative, Clarke's fixative, 4% formaldehyde, 4% formaldehyde+ 0.5% cetylpyridiniumchloride (F-CPC), 4% formaldehyde +0.1% glutaraldehyde gave unsatisfactory results. However, proteolytic digestion with pepsin of paraffin sections prior to staining of buffered formalin and F-CPCfixed material gave results comparable with those obtained on unfixed frozen sections as regards definition of the staining whereas staining intensity was decreased in some degree. No improvement was observed when using proteolytic digestion of tissue fixed in other fixatives.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00517142
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    The value of hyaluronidase treatment of different tissues before demonstration of fibronectin by the indirect immunoperoxidase technique (1982)
    Springer
    Histochemistry and cell biology 76 (1982), S. 517-525 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The influence of testicular hyaluronidase treatment on the immunohistochemical localization of fibronectin in different tissues (human articular cartilage, large intestine, synovial membrane and experimental granulation tissue) as well on frozen as on formaldehyde fixed, paraffin embedded tissue, has been studied using the indirect immunoperoxidase technique. Pretreatment with hyaluronidase is essential in demonstrating fibronectin in frozen sections of human articular cartilage. In the other tissues examined treatment with hyaluronidase was not essential, but gave a more optimal staining quality. The effect of hyaluronidase treatment was to some extent dependent on the duration of treatment. In formaldehyde fixed, paraffin processed tissue the improvement with hyaluronidase treatment was only seen when the hyaluronidase followed pepsin digestion of the deparaffinized tissue sections.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00489906
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    Demonstration of fibronectin in normal and injured aorta by an indirect immunoperoxidase technique (1983)
    Springer
    Histochemistry and cell biology 77 (1983), S. 395-403 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The presence and localization of fibronectin in normal and mechanically injured aorta in rabbits was studied using an indirect immunoperoxidase technique on tissue specimens fixed in formaldehyde, embedded in paraffin and pretreated with pepsin. The effect on staining quality of treatment with testicular hyaluronidase prior to immunoperoxidase staining was also examined. In the intima from normal aorta fibronectin was present in the subendothelial basal layer, along the internal and external elastic laminae, around and between the smooth muscle cells of the media and along the collagen and elastic fibres in the adventitia. Sixteen days after a single mechanical dilatation of the descending thoracic aorta all animals developed gross atherosclerotic-like changes. Microscopic examination revealed prominent neo-intimal hyperplasia with subendothelial, cushion-like thickenings but no medial or adventitial alterations. Fibronectin, in increased amounts, was found between and around the endothelial cells and in the subendothelial thickenings between the proliferating smooth muscle cells in relation to the fine, thin elastic and argyrophilic fibres. In the media and adventitia the amount and distribution of fibronectin was indistinguishable from uninjured control aortas. Treatment with testicular hyaluronidase before immunoperoxidase staining resulted in a higher staining resolution in normal and injured aorta. The conspicuous observation in the present study is that fibronectin exclusively accumulates in areas of tissue repair. The origins and functions of fibronectin during tissue injury and repair are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00490900
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    Paper (German National Licenses)
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  • 7
    Electronic Resource
    Electronic Resource
    Opsonic activity of fibronectin in the phagocytosis ofStaphylococcus aureus by polymorphonuclear leukocytes (1984)
    Springer
    European journal of clinical microbiology & infectious diseases 3 (1984), S. 108-112 
    Details
    ISSN: 1435-4373
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The effect of the opsonic activity of human purified fibronectin on phagocytosis ofStaphylococcus aureus by human polymorphonuclear leukocytes was investigated. After opsonization with fibronectin there was a significant increase in the rate of phagocytosis of four out of sixStaphylococcus aureus strains. Both attachment to and ingestion by leukocytes was affected, as revealed by lysostaphin treatment. Incubation of leukocytes with fibronectin prior to phagocytosis did not enhance the phagocytosis ofStaphylococcus aureus. This shows that the observed enhancement of phagocytosis was dependent on the binding of fibronectin toStaphylococcus aureus. The ability of fibronectin to opsonize different strains ofStaphylococcus aureus varied with the strain. A variation was also observed in fibronectin-induced phagocytosis by leukocytes from different donors.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02014326
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