ISSN:
1750-3841
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
The fine structure of laboratory purified broadbean and smooth pea starches, with an amylose content of 32-34%, has been studied by pullulanase debranching, before or after beta-amylolysis, and by the properties of the chemically fractionated amylose and amylopectin. The enzymatic study has shown the presence of the three chain populations (DP 〉 60, 45 and 15) observed with other starches. The linear DP 15 and 45 chains occur in a ratio of 8.5 for broadbean and 9.75 for pea, which indicates an amylopectin similar to cereal starches. The λmax, beta-amylolysis limit and intrinsic viscosity of the two amylopectins confirm the cereal-like nature. The two amylose components are not completely linear according to their beta-amylolysis limit of 81.5% which corroborates the in complete debranching of the total starch. The physical structure, studied by X-ray diffractometry, is of C-type. By submitting legume starch granules to mild acid hydrolysis (lintnerization), a residue has been obtained from both starches, which has an increase in the crystalline fraction, with a tendency towards the A-type pattern for broadbean and the B-type for pea. The crystallites are mainly formed of linear chains (CL 15) with some singly branched material (DP 25). Gelatinization of starch granules occurs at 44-65-86°C for broadbean with a heat of gelatinization of 3.8 cal g−1 and at 48-61-80°C for pea with a heat of gelatinization of 3.2 cal g−1.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1365-2621.1981.tb14537.x
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