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  • 1980-1984  (8)
Material
Years
Year
  • 1
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Macromolecules 13 (1980), S. 588-592 
    ISSN: 1520-5835
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Histochemistry and cell biology 77 (1983), S. 485-494 
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The progress of polyploidization in the human heart muscle cell was investigated by cytofluorometry, involving selective measurements of heart muscle cell nuclei. Thirty-two tissue samples, taken from the free wall of the left ventricle of each autopsied heart, were fixed in Carnoy's fluid. From thick (100–150 μm) paraffin sections, isolated cells for smears were obtained by enzyme digestion and ultrasonic treatment. The smears were stained with azocarmin G to eliminate background fluorescence and subsequently stained by an acriflavine-Feulgen reaction. Cytofluorometric DNA-determinations were carried out selectively on heart muscle cell nuclei, using the muscle striations revealed by azocarmin G-fluorescence as specific markers. The dynamic process of polyploidization in normal hearts could be divided into four stages. In the first stage (under 1 year of age), almost all heart muscle cell nuclei (94.3±1.8%) were diploid. In the second stage (1 to 9 years of age), the number of tetraploid nuclei increased (13.6±7.1%). In the third stage (9 to 22 years of age), octaploid nuclei first appeared and the number of tetraploid nuclei increased (26.7±3.9%). The DNA pattern in the fourth stage (22 to 75 years of age) was relatively constant, with a ratio of diploid (62.4±8.7%), tetraploid (31.4±6.7%) and octaploid (5.8±3.9%) nuclei. From these results it was concluded that physiological polyploidization progresses in proportion to the increase of heart weight. The frequency of polyploid nuclei in human heart was not so high as resported by previous investigators.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Histochemistry and cell biology 65 (1980), S. 269-276 
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The factors affecting fluorescence fading in cytofluorometry were investigated using different kinds of nuclear staining, mounting media, and procedure of specimen preparation. Acceleration of fluorescence fading was observed in smear specimens treated with RNase, trypsin, or hypotonic solution before pararosaniline Feulgen nuclear staining. Similar effect was found for other DNA-stainings such as “33258 Hoechst” and Feulgen reactions with different Schiff-type dyes, such as acriflavine-SO2 and cresylviolet-SO2, when chemically pure DNA was used. Fluorescence decay was rapid for all fluorochromes examined, when glycerin or buffer solution was used as mounting medium. Marked stabilization of fluorescence emission was induced in specimen mounted in non-fluorescent resin, Entellan (Merck), after post-staining fixation with absolute methanol for all tested fluorochromes. The same treatment induced almost complete fluorescence stabilization of fluorescein isothiocyanate (FITC); no detectable fluorescence fading was observed in a specimen stained with indirect immunofluorescence reaction using anti-UV-DNA antibody, during storage for 2 years at room temperature without special protection against light. These observations suggest that factors which bring about conformational stability of macromoleculedye complexes generally induce fluorescence stabilization.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A technique for isolation of cells from paraffin embedded tissue is indispensable for the performance of Feulgen-DNA cytofluorometry in parallel with the definition of histological characteristics. Background fluorescence due to nonspecific dye-binding by a “pseudo-plasmal reaction” is usually found to be so intense on cells isolated from formalin-fixed tissues, that we are often forced to abandon quantitative DNA determinations. In the present work, we report that fixation of tissues with Carnoy's fixative for 12 h at 5° C not only reduces nonspecific dye-binding but also facilitates the process of cell isolation. Furthermore, we find that pre-treatment of cells isolated from Carnoy-fixed tissues with acidic azocarmin G solution completely blocks nonspecific dye-binding in subsequent acriflavine Feulgen nuclear staining. This combination of techniques for specimen preparation enables us to carry out Feulgen-DNA cytofluorometry on cells isolated from histological sections with satisfactorily low coefficients of variation (less than 8%). The techniques should be widely applicable for parallel DNA determinations and histology.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In Feulgen nuclear staining nonspecific dye-binding due to the “pseudo-plasmal reaction” is intensified in isolated cells with intact cytoplasm, and cannot be eliminated by the post-irradiation method. Fluorescence intensity in the cytoplasm sometimes exceeds that of specific nuclear fluorescence, especially in brain and heart muscle cells, and it was almost impossible to perform cytofluorometric DNA quantification on such specimens. Various kinds of aldehyde-blocking agents such as sodium borohydride, 2,4-dinitrophenylhydrazine, aniline, and sodium pyrosulfite were effective in reducing the “pseudo-plasmal reaction”. But the blocking effects were not complete because of additional release of reactive aldehyde groups during subsequent Feulgen hydrolysis. Acidic azocarmin G produces a complete block of all “pseudo-plasmal reaction” in acriflavine-Feulgen nuclear staining, allowing accurate DNA-cytofluorometry to be carried out.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Human genetics 〈Berlin〉 54 (1980), S. 107-110 
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A low-power laser-UV microbeam of wave-length 257 nm was used for microirradiation of a small part of the nucleus of Chinese hamster cells. Following fixation in interphase or in the subsequent metaphase indirect immunofluorescent staining was performed with antiserum to photoproducts of DNA treated with far UV light. The results show that antibodies specific for UV-irradiated DNA can be used for a direct detection of laser-UV microirradiation-induced DNA photolesions. The potential usefulness of this method for investigation of the spatial arrangement of chromosomes in the interphase nucleus is discussed.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    GeoJournal 4 (1980), S. 125-132 
    ISSN: 1572-9893
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geography
    Notes: Abstract This review article is intended to introduce periglacial environment present and past in Japan, and to summarize some studies on it. The periglacial climate in Japan is characterized by a wide annual range of air temperature and much snow accumulation in winter. This makes for local variability in appearance of periglacial processes on the mountain slopes near crests. In Japan, the main interest in periglacial studies concerns the above-mentioned variability and its causes, that is, how periglacial processes relate to vegetation, local climate especially snow accumulation and its seasonal duration, soil, micro-relief, properties of bed rock etc. Seasonal soil freezing was measured in lowlands of Hokkaido. Depth of frost penetration without snow cover increases proportionally to the increments of accumulated degree-days. Depth of frost penetration on natural conditions is affected not only by the degree-days but also by the depth of snow cover which differs locally even within a small area. The oldest frozen ground phenomena was recognized in a horizon about 50,000 years old in the lowlands of Hokkaida. Involutions about 40,000 years ago exceeded active earth hummocks in size. And the phenomena reached the maximum phase is represented by icewedge casts of the En-a pumice (ca. 15,000 abp). Most of fossil features are covered with undisturbed Ta-d pumice (cz. 9,000 abp), but earth hummocks and vertical stones have been still formed in the Holocene tephra layers.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    GeoJournal 4 (1980), S. 125-132 
    ISSN: 1572-9893
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geography
    Notes: Abstract This review article is intended to introduce periglacial environment present and past in Japan, and to summarize some studies on it. The periglacial climate in Japan is characterized by a wide annual range of air temperature and much snow accumulation in winter. This makes for local variability in appearance of periglacial processes on the mountain slopes near crests. In Japan, the main interest in periglacial studies concerns the above-mentioned variability and its causes, that is, how periglacial processes relate to vegetation, local climate especially snow accumulation and its seasonal duration, soil, micro-relief, properties of bed rock etc. Seasonal soil freezing was measured in lowlands of Hokkaido. Depth of frost penetration without snow cover increases proportionally to the increments of accumulated degree-days. Depth of frost penetration on natural conditions is affected not only by the degree-days but also by the depth of snow cover which differs locally even within a small area. The oldest frozen ground phenomena was recognized in a horizon about 50,000 years old in the lowlands of Hokkaida. Involutions about 40,000 years ago exceeded active earth hummocks in size. And the phenomena reached the maximum phase is represented by icewedge casts of the En-a pumice (ca. 15,000 abp). Most of fossil features are covered with undisturbed Ta-d pumice (cz. 9,000 abp), but earth hummocks and vertical stones have been still formed in the Holocene tephra layers.
    Type of Medium: Electronic Resource
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