ISSN:
0377-0486
Keywords:
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
,
Physics
Notes:
The Raman spectra of L-histidine, L-histidine- 13C (labelled at C2 of the imidazole ring) and related compounds, namely, imidazole, 4-methylimidazole, N-acetyl-L-histidine methylamide, were observed in H2O and D2O solutions at various pH and pD values. A strong Raman band at around 1410 cm-1 was found for these compounds in acidic D2O solutions, and it was assigned to a mode characteristic of the N-deuterated imidazolium ring (Im+) in which the N1—C2—N3 symmetric stretching and N—D bending vibrations are mixed. It is concluded that this band would be useful as a probe for studying the ionization states of histidine residues in proteins.
Additional Material:
5 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jrs.1250120212
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