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  • 1980-1984  (6)
  • 1
    Electronic Resource
    Electronic Resource
    d-lactic acidosis in two patients with short bowel syndrome: Bacteriological analyses of the fecal flora (1982)
    Springer
    European journal of pediatrics 138 (1982), S. 324-326 
    Details
    ISSN: 1432-1076
    Keywords: d-lactic acidosis ; Short bowel syndrome ; Lactobacillus ; Kanamycin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Two cases of d-lactic acidosis associated with short bowel syndrome are described. The administration of kanamycin to the patients showed a decrease in d-lactate in blood and urine in parallel with disappearance of metabolic acidosis. Bacteriological analyses of the fecal flora showed an increase in Lactobacillus buchneri in the first patient and Lactobacillus fermenti IVa in the second; both bacteria were sensitive to kanamycin. Quantification of in vitro production of d-lactate by each species of bacteria isolated from the feces revealed that Lactobacillus produced more d-lactate than other species of bacteria. These observations indicate that Lactobacillus may play an important role in the induction of d-lactic acidosis in patients with short bowel syndrome.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00442509
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Epstein-Barr Virus-induced Lymphoblastoid Cell Lines from Patients with Primary Immunodeficiency Diseases (1980)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 11 (1980), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Peripheral lymphocytes from eight patients with congenital immunodeficiency diseases were infected with Epstein-Barr virus (EBV) in an attempt to establish B lymphoblastoid cell lines (LCL). These patients included three boys with congenital agammaglobulinaemia, two girls with hypogammaglobulinaemia, one boy with common variable immunodeficiency, one boy with severe combined immunodeficiency with adenosine deaminase deficiency, and one boy with DiGeorge syndrome. Five of the patients bore no surface immunoglobulins (slg) on their peripheral lymphocytes. LCL were established from seven of the eight patients. All the LCL established formed rosettes with EAC3 and had the ability to produce cytoplasmic immunoglobulins (clg) of various classes. Culture supernatants concentrated up to 100-fold developed precipitin bands by Ouchterlony's method with antisera to human Ig in all the established LCL. These results suggested that both slg-, clg- and C3+ cells and slg+, clg- and C3+ cells might be the target cells for EBV and that slg-, clg- and C3+ cells might be the precursor cells of B lymphocytes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1980.tb00221.x
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Further observations on the morphogenesis of human rotavirus in MA 104 cells (1984)
    Springer
    Archives of virology 79 (1984), S. 147-159 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Human rotavirus “KUN” strain was cultivated in a fetal rhesus monkey kidney cell line, MA 104 cells. Four types of virus particles in cells infected with KUN strain were clearly identified: nucleoid cores, single-shelled particles, double-shelled particles, and membrane band, “enveloped” particles. “Enveloped” particles were found only in the thin sections of infected cells. When first visible, the virus precursors appeared at the ribosome free membrane of rough endoplasmic reticulum (RER), increasing in size while simultaneously being coated with nucleocapsid, inner shell. Single-shelled particles were also synthesized within bundles of filaments of viroplasm in the cytoplasma. During subsequent virus maturation two types of “budding” processes were observed. Double-shelled particles arising at the RER membrane entered the cisternae of the RER through an exocytosis-like process. In contrast, the “enveloped” particles developed in the cisternae by being completely enclosed with RER membrane, and later during cytolysis released the single-shelled particles. These “enveloped” virus particles appeared to be the result of inefficient virus maturation at the last stage of outer capsid formation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01310808
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Effect of tunicamycin on human rotavirus morphogenesis and infectivity (1984)
    Springer
    Archives of virology 81 (1984), S. 363-369 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In the presence of tunicamycin, decreases in the number of double-shelled human rotavirus particles coincided with a reduction in cell lysates infectivity. The majority of particles within the endoplasmic reticulum were membrane bound, “enveloped” particles.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01310008
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Morphogenesis of human rotavirus type 2 Wa strain in MA 104 cells (1981)
    Springer
    Archives of virology 70 (1981), S. 33-41 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Morphogenesis of human rotavirus type 2 Wa strain in MA 104 cells was observed. The virus antigen in the cytoplasm was detected by indirect immunofluorescence twelve hours after infection. The cytopathic effect occurred 24 hours after infection when virus particles were detected by EM in the culture fluid as well as in thin sections of the infected cells. Virus particles were observed in the dilated RER, nuclear envelope (perinuclear space), viroplasm, and a lysosome-like body. Three types of virus particles were noted: double-shelled particles 75–85 nm in diameter, single-shelled particles 64–68 nm in diameter and electron-dense nucleoids or cores 32–40 nm in diameter. The outer shell of virus particles was acquired by budding through the membrane of the dilated RER. Tubular structures, similar in diameter to the single-shelled particles, were found in the cytoplasm and nucleus of infected MA 104 cells. Bundles of filaments or the leaflet-like inclusion bodies of membrane-bounded bundles of filaments were found in the cytoplasm and seemed to be associated with virus particles.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01320791
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    S-Adenosylhomocysteine hydrolase activity in a lymphoblastoid cell line from a patient with adenosine deaminase deficiency disease (1981)
    Springer
    Journal of inherited metabolic disease 4 (1981), S. 197-201 
    Details
    ISSN: 1573-2665
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract S-Adenosylhomocysteine (S-AdoHcy) hydrolase activity in a lymphoblastoid cell line from a patient with adenosine deaminase deficiency disease (ADA(−)LCL) was found to be approximately 60% of that in ADA(+)lymphoblastoid cell lines.S-AdoHcy hydrolase of ADA(−)LCL was more sensitive to inhibition by 2′-deoxyadenosine as compared with that of ADA(+)LCL. The inhibitory effect of 2′-deoxyadenosine was evident in cell growth and immunoglobulin production of lymphoblastoid cell lines.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02263651
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    Paper (German National Licenses)
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