ISSN:
1572-9931
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract A Vero cell line (Vero 153) resistant to 8-azaguanine and unresponsive to viral induction of interferon was isolated. This primate (African green) cell line was fused with mouse myeloma (S194/5) and normal human lymphocytes from peripheral blood. All Vero 153-mouse hybrids, 8 primary and 12 secondary clones, produced virus-induced mouse but not primate interferon. This occurred even in cultures where 〉90% of primate chromosomes were retained. Similarly 7 primary and 3 secondary Vero 153-human clones synthesized virus-induced Interferon. This could be neutralized by anti-human fibroblast (Β) but not by anti-human leukocyte (α) interferon antisera. The unresponsive nature of Vero 153 cells to interferon induction by viruses was not changed by the presence of interferon producing genomes from other cells. However, despite the inability to produce interferon, the Vero cell was able to play a role in the determination of the type ofinterferon made in the hybrid cell.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01543045
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