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  • 1
    Electronic Resource
    Electronic Resource
    Processing of Cricket paralysis virus induced polypeptides inDrosophila cells: Production of high molecular weight polypeptides by treatment with iodoacetamide (1981)
    Springer
    Archives of virology 68 (1981), S. 1-8 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Infection ofDrosophila cells with Cricket paralysis virus in the presence of Actinomycin D results in virtual complete inhibition of host cell protein synthesis by four hours post-infection. Using35S-methionine or14C-amino acids to pulse infected cells three major classes of viral induced proteins can be detected, (A) high molecular weight precursor proteins, (B) viral structural proteins and (C) low molecular weight cleavage products. The large number of high molecular weight proteins found in the infected cells suggests that a multiple cleavage cascade mechanism is partially utilized to produce virus structural proteins. In infected cells, even with short pulses, the largest viral induced protein obtained has a molecular weight of 144,000. However with pretreatment of the infected cells with iodoacetamide before pulsing, two further proteins are obtained with molecular weights of 205,000 and 190,000. Other changes occur in viral protein precursors in the presence of iodoacetamide.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01315161
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  • 2
    Electronic Resource
    Electronic Resource
    In vivo andin vitro synthesis of the proteins expressed by the RNA of black beetle virus (1981)
    Springer
    Archives of virology 69 (1981), S. 131-139 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Black beetle virus is a small RNA virus with a single capsid protein of molecular weight ⋍ 40,000. Two intracellular proteins, a presumed polymerase (molecular weight 110,000) and a protein having a molecular weight slightly greater than the capsid, were observed when infectedDrosophila cells were pulsed with35S-methionine. Viral RNA coded for the synthesis of several major proteins with molecular weights between 110,000 and 38,000 in rabbit reticulocyte lysate. Long chases of the translated products demonstrated processing of capsid protein precursors into capsid protein. Partial proteolysis demonstrated similarities in the proteins synthesisedin vitro andin vivo.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01315156
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    The proteins expressed by different isolates ofDrosophila C virus (1982)
    Springer
    Archives of virology 74 (1982), S. 21-30 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Isolates ofDrosophila C virus (DCV) fromDrosophila flies obtained in geographically different regions were adapted to growth inDrosophila tissue culture cells. The viruses, purified from tissue culture cells, were shown to be serologically related to one of the isolates (“O” from Ouarzazate, morocco). Analysis of the structural proteins by polyacrylamide gel electrophoresis demonstrated differences between the isolates. Labelling intracellular proteins of infectedDrosophila melanogaster cells with35S-methionine at 28° C demonstrated the presence of the virus structural proteins and their immediate precursors. Raising the temperature to 37° C both before and during the pulse period inhibited the processing of the high molecular weight proteins and resulted in a greater “shut-off” of host cell proteins than viral induced proteins. This allowed the precursor proteins to be compared as well as the structural proteins of the different strains. It was possible to clearly distinguish differences between the isolates on the basis of the induced proteins, although limited proteolysis of corresponding proteins showed marked similarities. Hence it is possible to distinguish between different isolates of the “same” small RNA-virus of insects from geographically different regions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01320779
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  • 4
    Electronic Resource
    Electronic Resource
    The intracellular proteins induced by cricket paralysis virus inDrosophila cells: The effect of protease inhibitors and amino acid analogues (1981)
    Springer
    Archives of virology 70 (1981), S. 1-9 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Treatment of Cricket paralysis virus infectedDrosophila cells with iodoacetamide before radiolabelling with35S-methionine results in the appearance of two high molecular weight polypeptides of ≃200,000 molecular weight, not apparent in untreated infected cells (17). To attempt to differentiate between the effects of iodoacetamide being attributable to either alteration of initial polyprotein or inhibition of the protease (either cellular or viral) the effects of a spectrum of protease inhibitors were examined. These included aprotinin, leupeptin, pepstatin, elevated zinc concentration, phenyl methyl sulphonyl-fluoride, N-tosyl-L-lysine chloromethyl ketone (TLCK) and N-tosyl-L-phenylalanine chloromethyl ketone (TPCK). TLCK and TPCK both inhibited the cleavage of proteins which demonstrates an inhibition of the protease activity. The introduction of amino acid analogues into the infected cells before pulsing also results in the appearance of higher molecular weight proteins. This could be attributed to alternation of the polyprotein making it nonsusceptible to digestion with pre-existing cellular protease or newly synthesized viral protease. The possibility that the presence of the amino acid analogues results in alteration of a viral coded protease cannot be eliminated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01320788
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  • 5
    Electronic Resource
    Electronic Resource
    In vitro translation of cricket paralysis virus RNA (1981)
    Springer
    Archives of virology 67 (1981), S. 175-180 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Cricket paralysis virus RNA acted as a messenger in a translation system and directed incorporation of35S-methionine into protein. Polyacrylamide gel analysis of the proteins demonstrated the presence of proteins of comparable molecular weight to the viral structural proteins and also potential high molecular weight precursors.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01318602
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  • 6
    Electronic Resource
    Electronic Resource
    The biochemistry of orbiviruses (1984)
    Springer
    Archives of virology 82 (1984), S. 1-18 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01309364
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  • 7
    Electronic Resource
    Electronic Resource
    Cell-free translation ofDrosophila C virus RNA: Identification of a virus protease activity involved in capsid protein synthesis and further studies onin vitro processing of Cricket paralysis virus specified proteins (1983)
    Springer
    Archives of virology 76 (1983), S. 101-115 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Drosophila C virus RNA acted as mRNA in rabbit reticulocyte lysates and directed the synthesis of at least one capsid protein and a number of higher molecular weight proteins. Kinetic analysis by pulse-chase experiments showed that a number of high molecular weight products acted as precursors to the capsid protein(s). Various dilution experiments were performed which showed that the virus specified a protease activity essential for the correct processing of precursors to give the capsid protein(s). A similar result was obtained with Cricket paralysis virus, and mixing experiments showed that the protease activity specified by one virus could perform some of the cleavages resulting in the production of the capsid proteins of the other virus. Some of the cleavages involving the highest molecular weight precursors could not be performed by the protease activity of the other virus. We could find no evidence for intramolecular cleavage of the capsids precursors of either of the viruses.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01311694
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  • 8
    Electronic Resource
    Electronic Resource
    The small RNA-viruses of insects (1982)
    Springer
    Archives of virology 72 (1982), S. 229-245 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01315220
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  • 9
    Electronic Resource
    Electronic Resource
    Presence of adrenal medullary chromaffin cells in primary adrenal dispersions and their persistence in long-term culture (1982)
    Springer
    Cell & tissue research 227 (1982), S. 225-229 
    Details
    ISSN: 1432-0878
    Keywords: Chromaffin cells ; Adrenocortical cell suspensions ; Cell culture ; Catecholamine fluorescence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Cell suspensions prepared by enzymatic dispersion of whole rat adrenal glands for the purpose of studying adrenocortical cells were found to contain chromaffin cells even though they are commonly thought to not survive in such preparations. These cells fluoresced when treated with methods specific for catecholamines. The fluorescent cells persisted in the cultures of “cortical” cells, took on the morphology of neurons in the cultures, maintained their specific catecholamine fluorescence in long-term cultures, and ultrastructurally were identical to chromaffin cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00206344
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