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  • 1
    Electronic Resource
    Electronic Resource
    Microdisc-electrophoretic study of deoxyribonucleases in cow snout epidermis (1983)
    Springer
    Archives of dermatological research 275 (1983), S. 213-217 
    Details
    ISSN: 1432-069X
    Keywords: Acid ; Neutral DNases ; Epidermis ; Microdisc ; Electrophoresis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Acid and neutral deoxyribonucleases (DNases) of the cow snout epidermis were investigated by the microdiscelectrophoresis of polyacrylamide gels containing highly polymerized DNA and by isoelectric focusing techniques. The nucleases were characterized with respect to their pH optimum. An acid DNase at pH 5.0 was detected as a single distinct band after the electrophoretic separation. After isoelectric focussing also, only one acid DNase activity with an isoelectric point (IP) of 6.2 was detectable. Neutral DNases at pH 7.4 were demonstrated as major and minor bands by their different electrophoretic mobilities. In the isoelectric focusing system also, two neutral DNases, a major one (IP, 4.6) and a minor one (IP, 6.4), were found. Characterization with respect to their histologic location showed acid and neutral DNases across the epidermal layers with the highest activities in the upper layers, where DNA concentration had been shown to be lowest. In correlation with their subcellular distribution, the highest activities of both acid and neutral DNase were found in the 105,00xg supernatant of the subcellular fractions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00416662
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  • 2
    Electronic Resource
    Electronic Resource
    Catalytic properties of ruthenium oxide on n-type semiconductors under illumination (1984)
    s.l. : American Chemical Society
    The @journal of physical chemistry 〈Washington, DC〉 88 (1984), S. 5214-5221 
    Details
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/j150666a020
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Electronic structure of a porphyrin solid film and energy transfer at the interface with a metal substrate (1980)
    s.l. : American Chemical Society
    The @journal of physical chemistry 〈Washington, DC〉 84 (1980), S. 751-756 
    Details
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/j100444a012
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  • 4
    Electronic Resource
    Electronic Resource
    Alterations in neutral amino acid transport across the blood-brain barrier in hepatic failure (1983)
    Springer
    Research in experimental medicine 183 (1983), S. 11-18 
    Details
    ISSN: 1433-8580
    Keywords: Blood-brain barrier ; Neutral amino acid ; Hepatic failure ; Amino acid transport into the brain
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The accelerated transport of the blood neutral amino acids into the brain in encephalopathic patients with fulminant hepatitis and advanced liver cirrhosis was demonstrated not only by determining the cerebrospinal fluid (CSF) aminogram but also by calculating the predicted velocity of the amino acid transport through the blood-brain barrier. Significant elevation in CSF aromatic amino acid (AAA) and methionine levels was observed in the encephalopathic patients. Arousal from hepatic encephalopathy by drip infusion of a branched chain amino acid (BCAA)-enriched solution was obtained coincidentally with the elevated BCAA levels and diminished concentrations of AAA and methionine in CSF. These clinical observations were confirmed experimentally in rats treated with carbon tetrachloride (CCl4) andd-galactosamine by obtaining the elevation of neutral amino acid contents in the brain and the slight increase in the brain uptake index (BUI) of a radiolabeled amino acid.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01851759
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  • 5
    Electronic Resource
    Electronic Resource
    Pleomorphism of hepatic regeneration (1984)
    Springer
    Research in experimental medicine 184 (1984), S. 49-57 
    Details
    ISSN: 1433-8580
    Keywords: Hepatic regeneration ; Partial hepatectomy ; Liver damage ; Repair of liver damage ; CCl4
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The pleomorphism of hepatic regeneration was studied in acute liver injury and partial hepatectomy of rats. Increases in3H-thymidine incorporation into the liver DNA fraction and in liver ornithine decarboxylase (ODC) activity were observed similarly in acute liver injury and following a two-thirds partial hepatectomy in normal rats. However, the increase in serum AFP levels was significant only in carbon tetrachloride (CCl4)-treated rats, and the portal-peripheral vein difference in plasma glucagon concentrations was great only in rats that underwent a partial hepatectomy. The weight of the remaining liver increased rapidly following a 67% hepatectomy in normal rats, but not in cirrhotic rats, of which three of five died within 6 days. Increases in liver prostaglandin E levels,14C-orotate incorporation into the liver RNA fraction and hepatic ODC activity were observed up to 10 h following a partial hepatectomy of normal liver but not after similar removal of cirrhotic liver. Three kinds of hepatic regeneration, i.e., normal and pathologic regeneration following surgical removal of either normal or injured liver and repair of liver damage following chemically induced liver deficiency, were proposed from the observations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01852222
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  • 6
    Electronic Resource
    Electronic Resource
    Influence of short-chain fatty acids and osmolality on mucin release in the rat colon (1981)
    Springer
    Cell & tissue research 219 (1981), S. 371-377 
    Details
    ISSN: 1432-0878
    Keywords: Short-chain fatty acids ; Osmolality ; Mucin release ; Colon (rat) ; Histochemistry ; Goblet cells ; Vacuolated cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The influence of short-chain fatty acids (SCFA) and osmolality on mucin release in the rat colon was studied histochemically by determining number of stained mucin-containing cells. SCFA did not significantly influence the number of cells staining for mucin. Hypertonic solutions (360 mosm/l) did not affect mucin release in the proximal colon, but stimulated mucin release in the distal colon. Solutions of lower osmolality (300 or 250 mosm/l) caused a considerable release of mucin from goblet cells as well as vacuolated cells in both the proximal and the distal colon; the lower the osmolality, the more mucin was released. The mucosa of the distal colon was conspicuously affected by solutions of lower osmolality. The influence of osmolality on mucin release was entirely local.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00210155
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  • 7
    Electronic Resource
    Electronic Resource
    Luminal mucin in the large intestine of mice, rats and guinea pigs (1981)
    Springer
    Cell & tissue research 219 (1981), S. 629-635 
    Details
    ISSN: 1432-0878
    Keywords: Cecum ; Colon ; Goblet cell ; Vacuolated cell ; Histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The luminal and epithelial mucin was studied histochemically in the large intestine of mice (Mus musculus), rats (Rattus rattus) and guinea pigs (Cavia porcellus) using freeze-substitution and vapor-fixation methods. Neutral mucin decreased and acid mucin increased in the epithelium from the cecum to the distal colon. Vacuolated cells contained more acid mucin than goblet cells. Luminal mucin always contained neutral mucin, which formed the main constituents in the cecum and in the proximal colon. Sialo-mucin increased from the cecum to the distal colon. Sulfo-mucin appeared only in the distal colon. Except in the cecum a luminal mucin layer (LML) was found at the epithelial surface. In the proximal colon LML was not entirely continuous and varied in composition and thickness (182.4 ± 170.1, 150.5 ± 110.4, 30.0 ± 28.9 (μm), in mice, rats and guinea pigs, respectively), and contained many bacteria. In the distal colon LML was compact, homogeneous and thin (33.6 ± 18.8, 16.1 ± 7.3, 29.1 ± 20.0 (μm), in mice, rats and guinea pigs, respectively) containing few bacteria. Possible functions of the luminal mucin and their regional differentiations were discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00210000
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