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  • 1
    Electronic Resource
    Electronic Resource
    Characterization of Catecholamine-Storage Organelles in Transplantable Phaeochromocytoma and Adrenal Glands of Rats (1982)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of neurochemistry 38 (1982), S. 0 
    Details
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract: The properties of the catecholamine-storing organelles from transplantable rat phaeochromocytoma and rat adrenal glands were compared by density gradient centrifugation. It was shown that tumour granules are more heterogeneous and less dense than adrenal granules. Both granule preparations can take up catecholamines and nucleotides by a process driven by an electrochemical proton gradient. Dopamine β-hydroxylase and glycoprotein III were analysed by immunological techniques. Glycoprotein III was shown to be a specific component of chromaffin granules. Tumour tissue (average weight 700 mg) contains amounts of these antigens comparable to those in 210 adrenals. The biosynthesis of granules in the tumour apparently occurs at a low rate, making turnover studies difficult. The transplantable rat phaeochromocytoma is very useful for studies on the uptake properties and the immunological characteristics of rat catecholamine storage granules because one tumour provides an amount of material that could otherwise be obtained only from a large number of adrenal glands.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1471-4159.1982.tb08675.x
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  • 2
    Electronic Resource
    Electronic Resource
    Dopamine β-Hydroxylase and Other Glycoproteins from the Soluble Content and the Membranes of Adrenal Chromaffin Granules: Isolation and Carbohydrate Analysis (1982)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of neurochemistry 38 (1982), S. 0 
    Details
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract: Chromogranin A and two other proteins (A1 and A2) of the soluble proteins of bovine chromaffin granules were isolated by extraction from polyacrylamide gels after electrophoresis. The carbohydrate content of these proteins was 5%, with galactose, N-acetylgalactosamine, and sialic acid as the main sugars. Membranes of chromaffin granules were solubilized with sodium dodecyl sulphate (SDS) and three glycoproteins were isolated by sequential affinity chromatography on Concanavalin A (Con A) and wheat germ lectin (WGL) Sepharose columns. Two glycoproteins, designated GP II and III, were found to have a high carbohydrate content of about 30%. Mannose, galactose, N-acetylgalactosamine, and sialic acid were the main sugars. In addition membrane-bound dopamine β-hydroxylase was isolated by this procedure. No significant differences between the carbohydrate composition of the membrane-bound and the soluble enzyme were revealed. It was shown that all four subunits of dopamine β-hydroxylase possess carbohydrate chains with an affinity for Con A. The isolation methods established in this study will be useful for immunological studies on these glycoproteins.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1471-4159.1982.tb08691.x
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  • 3
    Electronic Resource
    Electronic Resource
    Isolation and Immunological Characterization of a Glycoprotein from Adrenal Chromaffin Granules (1984)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of neurochemistry 42 (1984), S. 0 
    Details
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract: A glycoprotein (s-GP III) was isolated from the soluble lysate of chromaffin granules by chromatography with immunoaffinity and lectin columns. An identical protein (m-GP III) was shown to be present in the granule membranes. The apparent molecular weight of these glycoproteins as determined by the electrophoresis system of Laemmli (1970) was 43,000 under reducing conditions. In the absence of mercaptoethanol they aggregated to dimers. Antisera were raised against both the soluble and the membrane-bound forms of this glycoprotein. With these antisera GP III was further characterized: Immunoreplicas were obtained after two-dimensional electrophoresis of soluble and membrane-bound proteins of chromaffin granules. GP III was identified as a protein with a rather broad pI (4.6-5.3), indicating microhetero-geneity. As shown by subcellular fractionation, m-GP HI is specifically confined to chromaffin granules. GP III can therefore be used as a marker for the membranes of these organelles. The soluble form is secreted from adrenal medulla during stimulation with carbamylcholine chloride. An immunologically identical antigen was detected in adeno- and neurohypophysis. The physiological function of GP III is still unknown. It does not demonstrate any of the enzymatic activities so far known to occur in chromaffin granules.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1471-4159.1984.tb12704.x
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  • 4
    Electronic Resource
    Electronic Resource
    Uptake of Nucleotides and Catecholamines by Chromaffin Granules from Pig and Horse Adrenal Medulla (1980)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of neurochemistry 35 (1980), S. 0 
    Details
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract: The uptake of nucleotides and Catecholamines into chromaffin granules from adrenals of pigs and horses is similar to that previously seen in bovine chromaffin granules. The rate of [3H]ATP uptake at 2 mM-ATP concentration was 0.42 ± 0.06 and 0.15 ± 0.02 nmol/mg protein/min for pig and horse granules, respectively. The apparent Km's were 1.37 mM for pig granules, 0.89 mM for horse granules, and 1.2 mM for ox granules. The sensitivity of the uptake for nucleotides and catecholamine to specific inhibitors was found to be similar in granules from pig and ox, indicating that the same mechanisms of uptake are involved in both species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1471-4159.1980.tb12516.x
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  • 5
    Electronic Resource
    Electronic Resource
    The Proteins of Catecholamine-storing Organelles (1982)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 15 (1982), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1982.tb03759.x
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  • 6
    Electronic Resource
    Electronic Resource
    Exposure of an antigen of chromaffin granules on cell surface during exocytosis (1983)
    [s.l.] : Nature Publishing Group
    Nature 301 (1983), S. 610-611 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Rabbit antisera were raised against glycoprotein III (GP III) isolated from bovine chromaffin granules as described previously7. This antigen is specifically confined to chromaffin granules and its antigenic groups are present on the inner side of the granule membrane facing the interior (R.F.-C. ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/301610a0
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  • 7
    Electronic Resource
    Electronic Resource
    Pyruvate kinase “Göttingen1,2”: Congenital hemolytic anemia, evidence of double heterozygosity, and lack of enzyme cooperativity (1982)
    Springer
    Human genetics 〈Berlin〉 60 (1982), S. 381-386 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Double heterozygosity of pyruvate kinase (PK) deficiency associated with hereditary hemolytic anemia is emphasized by studies of a kindred harboring two distinct mutant forms of this enzyme. The hematologically unaffected parents exhibit slightly reduced PK activity, a normal Hill coefficient, and a normal thermodynamic dissociation constant for the overall reaction. The paternal enzyme is characterized by normal substrate affinities and decreased activities with the substrate analogues CDP and GDP, whereas the maternal enzyme shows normal affinity for PEP, but an increased affinity for ADP and low thermostability. It is assumed that the erythrocytes of the parents contain a mixture of normal PK and a functionally abnormal isoenzyme, the latter differing between the parents. The two children suffer from hereditary hemolytic anemia. Their PK must be a combination of the mutant paternal and maternal isoenzymes, and their activities are reduced to about 30%. These enzymes are characterized by an increased affinity for PEP and a decreased affinity for ADP, a Hill coefficient of about 1 (indicating lack of cooperativity due to a loss of its allosteric properties), a decreased overall catalytic activity, and a higher resistance to heat denaturation. Further differences are observed in the SDS-gel electrophoresis between the two patients' enzymes. From the enzymological point of view it is impossible to characterize true PK variants in such double heterozygous cases which contain a combination of two different isoenzymes. The cause of chronic hemolysis appears to depend mainly on the loss of the allosteric properties, i.e., the lack of enzyme cooperativity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00569226
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  • 8
    Electronic Resource
    Electronic Resource
    Erythrocyte pyruvate kinase deficiency: Characterization of a new variant (PK “Aarau”) (1984)
    Springer
    Annals of hematology 48 (1984), S. 123-129 
    Details
    ISSN: 1432-0584
    Keywords: Pyruvate kinase deficiency ; Allosteric regulation ; SDS-gel electrophoresis ; Erythrocyte glycolytic intermediates
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A new PK variant with moderate hemolytic anemia is described. The enzymes of the nonanemic parents show sigmoidal reaction kinetics, with normal kinetic parameters, but differ with respect to nucleotide specificity, thermostability, and the concentrations of the glycolytic intermediates in the erythrocytes. The most characteristic features of the patient's (daughter) enzyme are a 30% activity, hyperbolic reaction kinetics and only two bands in the SDS-gel electrophoresis instead of three bands observed with the parental enzymes. Moreover, the pH-optimum is shifted to the acidic range, the affinity for PEP and ADP is decreased, ATP inhibition is negligible and FDP-activation is roughly ten times smaller than with controls. The concentrations of 2.3-DPG, 2-PG and PEP in the erythrocyte are increased, but ATP decreased. As there is no consanguinity in the parents and their enzymes are different this PK mutant can be considered to be compound-heterozygous for two different mutant PK alleles.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00320334
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  • 9
    Electronic Resource
    Electronic Resource
    Elektrische Eigenschaften polymerer Festkörper-Physikalische Grundlagen und technische Anwendungen. Teil II. Polymere Isolatoren und DielektrikaTeil I: Acta Polymerica 34 (1983) 278.Die einzelnen Abschnitte wurden von folgenden Autoren bearbeitet: L. Brehmer (1., 5. und 6.), M. Eberhardt (2.), K. D. Konietzko, J. Böhm und L. Röhr (3.), C. Lerch und H. Winkler (4.). Gesamtredaktion: L. Brehmer (1983)
    Weinheim : Wiley-Blackwell
    Acta Polymerica 34 (1983), S. 287-298 
    Details
    ISSN: 0323-7648
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Description / Table of Contents: The relationships between charge carrier transport and breakdown mechanism are summarily discussed and essential aspects of the electric breakdown in solid polymer insulating materials, particularly the effect of defects on the field strength at breakdown and the life time characteristics, are considered. The most important polymers applied in cable insulation are characterized with respect to their parameters and their application facilities are explained.
    Notes: Die Zusammenhänge zwischen Ladungstransport- und Durchschlagmechanismen werden zusammenfassend eingeschätzt und wesentliche Aspekte des elektrischen Durchschlagverhaltens polymerer Feststoffisolierungen dargestellt. Dabei wird besonders auf den Einfluß von Fehlstellen, auf die Durchschlagfeldstärke und die Lebensdauerkennlinien eingegangen. Die für den Einsatz in der Kabelisoliertechnik wichtigsten Polymere werden bezüglich ihrer Materialkenndaten charakterisiert und ihr Einsatz in der Kabeltechnik erläutert. Ferner werden die Kenndaten wichtiger polymerer Dielektrika und die Vorteile und Grenzen ihres Einsatzes als Kondensatorfolie zusammengefaßt.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/actp.1983.010340509
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  • 10
    Electronic Resource
    Electronic Resource
    Inspection of the heat exchangers of the reactor saphir by Acoustic EmissionVorgetragen in Edinburg: “Symposium on Structural Faults: Inspection and Repair” (22-24 March, 1983) (1984)
    Weinheim : Wiley-Blackwell
    Materialwissenschaft und Werkstofftechnik 15 (1984), S. 246-250 
    Details
    ISSN: 0933-5137
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Description / Table of Contents: Schallemissionsprüfung von Wärmetauschern des Reaktors SaphirDie Schallemissionsanalyse ist eine zerstörungsfreie Prüfmethode, die zur Untersuchung und Überwachung von Strukturen und druckführenden Komponenten verwendet wird. Das Ziel der Messung am 5 MW Forschungsreaktor Saphir war die Untersuchung und Auswertung der hohen Betriebsgeräusche in den Wärmetauschern des Kühl-systems.Die Ergebnisse zeigten, daß von den 9 Wärmetauschern, die in 3 Gruppen angeordnet sind, 5 Wärmetauscher ein hohes Betriebsgeräusch aufweisen. Die Ursache ist auf die Schwingung der Rohrbündel in Wärmetauschern beim Durchfluß des Pool-Wassers zurückzuführen. Die axiale Ortung bestätigte, daß die Geräusche von mehreren Quellen verursacht werden.
    Notes: The acoustic emission technique is a non-destructive testing method used for inspection and surveillance of structures and pressurized components. In particular, the purpose of the measurement on the 5 MW research reactor SAPHIR was to investigate and evaluate the abnormally high noise levels during operation of the heat exchangers of the cooling system.The results showed that 5 heat exchangers out of the total of 9 (assembled in 3 groups) were responsible for the higher noise level. This is probably due to some abnormal vibration of the tube bundles therein, caused by the through-flow of the pool water. Indeed, attempts of localizing the source showed that there must be several, distributed sources.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mawe.19840150705
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