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  • 1
    Digitale Medien
    Digitale Medien
    Intracellular localization of actin with fluorescently labelled heavy meromyosin (1975)
    Springer
    Cell & tissue research 161 (1975), S. 431-444 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Actin ; Fluorescent heavy meromyosin ; Non-muscle cells ; Motility ; Light microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary An improved technique for fluorescent labelling of heavy meromyosin has made it possible to detect on a light microscopic level the cellular sites of actin localization. Rabbit heavy meromyosin (HMM) was labelled with fluorescein isothiocyanate so that the actin binding site was protected during the reaction. The specificity of fluorescent HMM binding to cellular actin was tested by using glycerinated myofibrils. Staining was most intense in the I-Bands, and decreased at the edges of the A-Band, where actin filaments overlap with myosin. No staining occurred in the H-Zones or in the Z-Bands. The fluorescent HMM could be removed by washing with a relaxing solution. Similar sarcomeric patterns were obtained when embryonic chick skeletal and cardiac muscle cells were stained with fluorescent HMM. Localized fluorescent staining was also observed in smooth muscle fibers, axons and growth cones of nerves, acrosomal caps of sperm, cleavage furrows of dividing cells and pseudopods of various motile cells, all of which are known to contain actin. In sessile cells, the actin was found predominantly in fibrous bundles. This pattern of actin localization was altered when the cells underwent cleavage or became motile. The relationship between the intracellular distribution of actin and its function in the cell is discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00224134
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  • 2
    Digitale Medien
    Digitale Medien
    Cell surface changes during mitosis and cytokinesis of epithelial cells (1984)
    Springer
    Cell & tissue research 237 (1984), S. 409-417 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Mitosis ; Cytokinesis ; Microvilli ; Scanning electron microscopy ; Cell surface
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary PtK2 cells were studied with scanning electron microscopy to record changes on the cell surface during mitosis and cytokinesis. During prophase, prometaphase and metaphase, the cells remain very flat with few microvilli on their surfaces. In anaphase cells, there is a marked increase in the number of microvilli, most of which are clumped over the separating chromosomes and polar regions of the mitotic spindle leaving the surface of the interzonal spindle region relatively smooth. Microvilli appear over the interzonal spindle region in telophase and the cells also increase in height. At the beginning of cleavage, the distribution of microvilli is roughly uniform over the surface but it becomes asymmetric at the completion of cleav-age when the daughter cells begin to spread. At this time most microvilli are over the daughter nuclei and the surfaces that border the former cleavage furrow. The regions of the daughter cells distal to the furrow are the first to spread and their surfaces have very few microvilli. When chromosome movement is inhibited by either Nocodazole or Taxol, microvilli formation is inhibited on the arrested cells. Nevertheless cell rounding still takes place in the normal time period. It is concluded from these observations that the signal for the onset of chromosome movement in anaphase is accompanied by a signal for the formation of microvilli. It is suggested that there is also a separate signal for the cell-rounding event in mitosis and that microvilli do not play a role in this contractile process.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00228425
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  • 3
    Digitale Medien
    Digitale Medien
    Formation of myofibrils in spreading chick cardiac myocytes (1984)
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 4 (1984), S. 405-416 
    Details
    ISSN: 0886-1544
    Schlagwort(e): cardiac muscle ; myofibril ; cell spreading ; Z bands ; alpha-actinin ; Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Medizin
    Notizen: Cardiac myocytes were isolated from 5-6-day-old chick embryos and allowed to spread in culture. The distribution of alpha-actinin in the cells was followed for five days in culture by exposing permeabilized cells to rhodamine-labeled alpha-actinin and also by injecting the labeled alpha-actinin into living myocytes. In addition to labeling the Z bands of sarcomeres, the added alpha-actinin also labeled small particles that were usually arranged periodically in linear arrays with a spacing between particles of 0.3-2.0 μm. Actin was localized between the particles of alpha-actinin by means of fluorescein-labeled heavy meromyosin. The punctate localization of alpha-actinin was prominent in pseudopods, behind ruffles, and at the periphery of spreading cells. Long rows of particles of alpha-actinin were often parallel to one another with the alpha-actinin particles in register. These linear arrays appeared to merge laterally to form strands with broader concentrations of alpha-actinin. Other linear arrays were parallel to myofibrils in the cell and some extended outward from the ends of myofibrils. We conclude that during spreading of cardiac myocytes, myofibrils form at the cell periphery behind the extending margins of the cell, and that the aggregates of alpha-actinin found in these areas are nascent Z bands in the forming myofibrils.
    Zusätzliches Material: 7 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/cm.970040602
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  • 4
    Digitale Medien
    Digitale Medien
    Microinjection of Lucifer yellow CH into sea urchin eggs and embryos (1983)
    Springer
    Cell & tissue research 234 (1983), S. 309-318 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Division ; Dye coupling ; Development ; Embryo ; Microinjection
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Eggs and embryos of Arbacia punctulata were microinjected with the fluorescent dye, Lucifer yellow CH, using a simple pressure injection system. When injected into eggs that were subsequently fertilized, the dye was distributed throughout all cells of the developing embryo. If one cell of a two-cell embryo was injected, dye did not diffuse into the uninjected blastomere. During subsequent development, all progeny of the injected cell contained dye resulting in an embryo that was half-fluorescent. Blue light irradiation of a two-cell embryo, one cell of which had been injected with Lucifer yellow, caused the injected blastomere to stop further divisions while the uninjected blastomere developed normally and was free of dye. These results indicate that the first two blastomeres of Arbacia embryos are not electrically coupled, nor up to the time of hatching, is there any coupling between cells in one half of the first cleavage plane and cells in the other half.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00213770
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  • 5
    Digitale Medien
    Digitale Medien
    Close apposition of muscle cells in the longitudinal bands of the body wall of a holothurian, Isostichopus badionotus (1982)
    Springer
    Cell & tissue research 227 (1982), S. 465-473 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Electron microscopy ; Junctions ; Smooth muscle ; Echinodermata ; Holothuria, Aspidochirotida
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Electron microscopy reveals that sarcolemmata of adjacent muscle cells form pentalaminar junctions by fusion of apposed trilaminar double leaflet membranes. These junctions appear to be candidates for low resistance pathways between muscle fibers. The muscles depolarize slowly when bathed in solutions containing elevated concentrations of KCl, and the sucrose gap method can then be used to measure the potential difference between polarized and depolarized regions. Thus the junctions which we have observed may provide the structural basis for electrical transmission through the sucrose gap.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00204778
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  • 6
    Digitale Medien
    Digitale Medien
    Reduction of density and anisotropic distribution of intermediate filaments occur during avian skeletal myogenesis (1984)
    New York, NY [u.a.] : Wiley-Blackwell
    American Journal of Anatomy 171 (1984), S. 427-440 
    Details
    ISSN: 0002-9106
    Schlagwort(e): Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Medizin
    Notizen: Chicken skeletal muscle taken from embryos in ovo was examined by thin-section electron microscopy. Measurements of filament diameters reveal three nonoverlapping groups of filaments: thin (actin myofibrillar) filaments with mean diameters of 5.3 ± 0.6 nm (S.D.), thick (myosin myofibrillar) filaments with mean diameters of 15 ± 1.4 nm, and intermediate filaments with mean diameters of 9.3 ± 0.9 nm. During muscle development these diameters do not change. By counting the number of filaments observed in the sarcoplasm at different stages, we find that the spatial density of intermediate filaments decreases during avian myogenesis in ovo, from 91 intermediate filaments/μm2 at 6 days to 43 intermediate filaments/μm2 at 17 days in ovo. Initially randomly arranged, some intermediate filaments become associated with Z discs, sarcoplasmic reticulum, nuclear membrane, and the sarcolemma between 6 and 10 days in ovo. These associated intermediate filaments course both parallel and transverse to myofibrils, forming lateral connections between myofibrillar Z discs and longitudinal connections from Z disc to Z disc within myofibrils. Intermediate filaments also appear to connect Z discs with the nuclear membrane. The intermediate filament associations persist through day 17 of development, after which the presence of cytoskeletal filaments is obscured by the densely packed myofibrils and membranes. Intermediate filament distribution becomes anisotropic during development. A greater proportion of intermediate filaments in the immediate perimyofibrillar area are oriented parallel to myofibrils than in other areas, so that the majority of the intermediate filaments nearest the myofibrils course parallel to them. The longitudinal intramyofibrillar intermediate filaments persist throughout development, as shown by their existence in KI-extracted adult myofibrils.
    Zusätzliches Material: 9 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/aja.1001710407
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