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  • 1
    Electronic Resource
    Electronic Resource
    Morphology and biochemistry of small, intensely fluorescent cells of sympathetic ganglia (1975)
    [s.l.] : Nature Publishing Group
    Nature 256 (1975), S. 315-317 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Studies (T.C. and T.H.W., unpublished) have shown that the morphology of the sympathetic interneurone is considerably more complex and shows more species diversity than is sometimes appreciated, which raises the possibility ?of major species differences in its 'biochemistry and in the mechanisms ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/256315a0
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    A method for studying glyoxylic acid induced fluorescence and ultrastructure of monoamine neurons (1976)
    Springer
    Histochemistry and cell biology 49 (1976), S. 95-106 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Inasmuch as precise correlations of light- and electronmicroscopy are crucial for understanding biostructure, it seemed necessary to bring together the advantages of the glyoxylic acid (GA) method (for inducing monoamine fluorescence) and electron microscopy. A combined fluorescence and electron microscope method using GA is introduced. The brain is perfused by 2% GA in Krebs-Ringer bicarbonate buffer (pH 7.0) and this solution is followed by 4% paraformaldehyde containing 0.5% glutaraldehyde in Sorensen's phosphate buffer (pH 7.4). Sections are cut by cryostat or by vibratome and incubated in 2% GA in phosphate buffer (pH 7.0). Using fluorescence microscopy, features of interest are sketched and/or photographed. Afterwards, the same or subsequent section is processed for electron microscopy. Since axons of catecholamine-containing neurons (as well as their perikarya and terminals) are visualized by GA, the recommended procedure expands the range of studies concerning monoamine neurons that can now be carried out effectively.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00495673
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Histofluorescence characteristics and quantification of small intensely fluorescent (SIF) cells in sympathetic ganglia of several species (1975)
    Springer
    Cell & tissue research 162 (1975), S. 331-341 
    Details
    ISSN: 1432-0878
    Keywords: Sympathetic ganglia ; SIF cells ; Species variation ; Cell types ; Monoamine fluorescence method
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Small intensily fluorescent (SIF) cells in the superior cervical ganglion of the cow, cat, rabbit, rat, guinea pig and monkey were studied, using the glyoxylic acid monoamine fluorescence method. SIF cell populations per mg ganglion tissue showed great species variation. The greatest numbers of SIF cells per mg were found in the rat (380±30 per mg). Intermediate numbers (76±20 per mg) were found in the guinea pig; and SIF cells in other species were much more sparsely distributed (less than 10 per mg). Two types of SIF cell were identified. Type I cells have long (up to 200 μ) processes which ramify among the principal ganglionic neurons, and this type often occurs singly; whereas type II cells tend to occur in clusters near blood vessels in the interstitial or subcapsular regions of the ganglion. As a general hypothesis we propose that type I SIF cells are interneurons whereas type II SIF cells operate through a neurosecretory mechanism.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00220179
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    Paper (German National Licenses)
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