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  • 1975-1979  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    International journal of immunogenetics 3 (1976), S. 0 
    ISSN: 1744-313X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Salivas from group B secretor or non-secretor, acting on O red cells in the presence of UDP-galactose, each converted them into B active cells, which were agglutinated against anti-B human serum (1:512) at the titre of thirty-two-fold, while secretor or non-secretor group AB salivas converted O red cells into B active cells, which were agglutinated by anti-B human serum (1:512) at the titre of eight- to sixteen-fold. The results indicate that the α-galactosyltransferases which participate in the biosynthesis of group B substance are secreted in group B or AB salivas of both secretor and non-secretor types as well as in their sera.Agglutinabilities of enzymatically converted B-active red cells against anti-B human serum indicate that α-galactosyltransferase activities of both serum and saliva from a weak B (Bw) individual, who has weak B antigens in red cells and saliva, were lower than those of normal group B.The α-galactosyltransferase activities in group Bm sera were lower than those of normal group B, while the enzyme activities in salivas of group Bm were demonstrated to the same degree in normal group B salivas.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of neurochemistry 30 (1978), S. 0 
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract— A simple fluorometric method for the estimation of endogenous 3,4-dihydroxyphenylethylene glycol sulphate (DOPEG-SO4) in brain tissue is described. DOPEG-SO4 was extracted from brain tissue with 0.4 n-perchloric acid and purified by column chromatography on QAE-Sephadex A-25, Cl− form. The isolated DOPEG-SO4 was hydrolysed by incubation with a purified preparation of sulphatase from Helix pomatia. The free DOPEG thus formed was further purified on a column of aluminium oxide and determined fluorometrically after conversion to a fluorescent compound by condensation with ethylenediamine.The sensitivity of this method was about 15 ng per perchloric acid extract applied on the column (3 ml) and the reproducibility was excellent, with a variation coefficient of 1.6% (n = 5). The concentration of DOPEG-SO4 in whole brains of rats, determined by the present method, was 79.3 ± 4.7 ng/g (mean ± S.D., n = 10) in terms of free DOPEG. No appreciable DOPEG-SO, was detected in the brains of mice and guinea-pigs.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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