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  • 1
    Digitale Medien
    Digitale Medien
    Cell wall enzymatic lysis of the yeast form of Pullularia pullulans and wall regeneration by protoplasts (1975)
    Springer
    Archives of microbiology 104 (1975), S. 271-277 
    Details
    ISSN: 1432-072X
    Schlagwort(e): Protoplasts ; Regeneration ; Wall Structure ; Pullularia ; Ultrastructure ; Membrane Splitting ; Aberrant Tubes
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract During the process of degradation of the cell wall of the yeast form of Pullularia pullulans by the lytic system of Micromonospora chalcea samples were withdrawn at different times and observed under phase contrast and electron microscope. The progressive lysis of the walls reveals a fibrillar component inside the apparently amorphous wall. Freeze etched preparations of cells during the formation and regeneration of protoplasts show that the cellular membrane is split and this method allows the smooth external face of the membrane and other internal face covered by particles to be seen. The fact that the smooth face of the membrane is only visible during the preparation or the regeneration of protoplasts and very rarely when intact cells are fractured, suggests a strong adherence between cell wall and this external layer of the membrane. During the regeneration which takes place as in most of the yeasts and moulds, a special study of the extension of the cell wall is made and a possible mechanism for this extension of the regenerated cell wall is proposed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00447336
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Protoplasts of Trichoderma viride (1975)
    Springer
    Archives of microbiology 103 (1975), S. 199-203 
    Details
    ISSN: 1432-072X
    Schlagwort(e): Trichoderma viride ; Fungal Protoplasts ; Protoplasts Formation ; Protoplasts Regeneration ; Abortive Tubes ; Lytic System ; Streptomyces venezuelae RA ; Micromonospora chalcea
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract High yields of protoplasts from the 18-hr old mycelium of Trichoderma viride were obtained by using the lytic system, produced by Streptomyces venezuelae RA and Micromonospora chalcea grown on a synthetic medium containing laminarin and chitin, when 0.7 M MgSO4 or (NH4)2SO4 were used as osmotic stabilizers. Regeneration of these protoplasts occurred through the production of an abortive tube and direct germination of the protoplasts. Regeneration could also take place in the medium used to produce protoplasts, but the process was different in many details.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00436350
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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    Artikel (Nationallizenzen)
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