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  • 1
    Digitale Medien
    Digitale Medien
    Isolation of bovine zonae pellucidae from ovaries with collagenase: Antigenic and sperm receptor properties (1979)
    New York, NY : Wiley-Blackwell
    Gamete Research 2 (1979), S. 187-192 
    Details
    ISSN: 0148-7280
    Schlagwort(e): zona isolation ; collagenase ; receptor for sperm ; Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie
    Notizen: Zonae pellucidae were collected from bovine ovaries by chopping, dispersing the chopped tissue with collagenase, sieving through nylon mesh screens, and pipetting. The zonae were free of corona cell processes when examined under the scanning electron microscope. Solutions of zonae obtained with collagenase exhibited the same antigenic and sperm receptor properties as those obtained without enzyme treatment.
    Zusätzliches Material: 3 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/mrd.1120020210
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Bovine and hamster zona solutions exhibit receptor activity for capacitated but not for noncapacitated sperm (1978)
    New York, NY : Wiley-Blackwell
    Gamete Research 1 (1978), S. 259-263 
    Details
    ISSN: 0148-7280
    Schlagwort(e): solubilized zona ; receptor ; capacitation ; hamster ; cow ; Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie
    Notizen: Hamster and bovine zonae pellucidae were dissolved by heating them in buffered saline for 35 min at 65°C and 80°C, respectively. Pretreatment of noncapacitated sperm with these solutions did not affect their binding to eggs, but the binding of cumulus-capacitated hamster sperm and of bovine sperm that had undergone a capacitation-like surface change in response to hypertonic-treatment was severely curtailed. These experiments demonstrate that such zona solutions exhibit receptor activity, but only for the capacitated sperm.
    Zusätzliches Material: 3 Tab.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/mrd.1120010305
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    Immunization of female rabbits with heat-solubilized bovine zonae: Production of anti-zona antibody and inhibition of fertility (1978)
    New York, NY : Wiley-Blackwell
    Gamete Research 1 (1978), S. 19-26 
    Details
    ISSN: 0148-7280
    Schlagwort(e): zona pellucida ; contraception ; Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie
    Notizen: Bovine zonae were solubilized by heating to 80°C in buffered saline. On injection of female rabbits with this solution, zona-specific antisera were produced that could be assayed on bovine eggs in vitro by immunofluorescence, coating, and inhibition of sperm-binding. When the latter was complete, the rabbits were found to be infertile. Immunofluorescence assays showed that the rabbit anti-bovine zona serum reacted as strongly with rabbit and rhesus monkey zonae as with homologous zonae. Strong cross-reaction was also observed with marmoset and dog zonae, but with hamster zonae the reaction was relatively weak.
    Zusätzliches Material: 3 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/mrd.1120010105
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 4
    Digitale Medien
    Digitale Medien
    Localization of zona antigen in bovine ovary sections by fluorescent antibody (1979)
    New York, NY : Wiley-Blackwell
    Gamete Research 2 (1979), S. 201-206 
    Details
    ISSN: 0148-7280
    Schlagwort(e): zona pellucida ; antibody ; ovary ; calf ; Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie
    Notizen: Treatment of bovine ovary sections with rabbit antibovine zona serum followed by fluorescein-conjugated goat antirabbit IgG produced a specific fluorescent staining only of the zonae pellucidae. Fluorescence was greater near the inner and outer surface of the zona, suggesting that either the same antigen occurs in higher concentration in these regions or that there is more than one antigen, the most immunogenic being located peripherally. In some atretic follicles fluorescent material appeared to diffuse into the degenerating oocyte and into the intercellular spaces of the cumulus oophorus.
    Zusätzliches Material: 3 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/mrd.1120020303
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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