Electronic Resource
Oxford, UK
:
Blackwell Publishing Ltd
Physiologia plantarum
34 (1975), S. 0
ISSN:
1399-3054
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Biology
Notes:
A method is described for the isolation, purification and quantitation of free cytokinin bases and ribosides using ethyl acetate at pH 7.7 for the extraction. The extraction is almost complete (97.7%) as determined by using N6-(Δ2-isopentenyl)adenine-8-14C. The subsequent fast purification by chromatography on a standardized silica gel column in chloroform-methanol (7:3 v/v) is followed by thin layer chromatography (silica gel 60 F254) in chloroform-acetic acid (8:2 v/v). The recovery of N6-(Δ2-isopentenyl)adenine-8-14C after this two step purification was 78–82%. The efficiency of the method was determined by applying this procedure to N6-(Δ2-isopentenyl)adenine and N6(Δ2-isopentenyl)adenosine. Using gas liquid chromatography the recovery for N6-(Δ2-isopentenyl)adenosine was determined to be 61% and compared to 43% for N6-(Δ2-isopentenyl)adenosine, showing the suitability of the described method for gas liquid chromatography.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1399-3054.1975.tb03822.x
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