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  • 1975-1979  (3)
  • 1
    Electronic Resource
    Electronic Resource
    Initial Attempt to Develop an AFP Gene Regulation Analysis System (1978)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 8 (1978), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: This is a preliminary report on methods being developed for the isolation of rat AFP messenger RNA and the synthesis of DNA complementary to this mRNA. Using Morris hepatoma 7777 as source material, AFP-synthesizing polysomes have been isolated by indirect immunoprecipitation. The 18S Poly A + RNA (putative mRNAAFP) recovered from these polysomes by differential centrifugation and oligo-dT cellulose chromatography, was translated in a reticulocyte cell-free system into a peptide immunoprecipitable by anti-AFP sera (and not by anti-albumin sera) and co-migrating with native AFP on polyacrylamide gel electrophoresis. DNA complementary to the mRNA was synthesized by the use of avian myeloblastosis virus reverse transcriptase. The cDNA probe is highly homogeneous, as judged by alkaline sucrose gradient sedimentation, and yields a 1/2 C,t value of 8.5 times 10-3 with its template. Preliminary hybridization experiments indicate that sequences hybridizable to the cDNA probe constitute 3.0%, 0.5%, and 0.008% of the total poly A + polysomal RNA populations of 13 day old rat liver, Morris hepatoma 7777 and adult rat liver, respectively.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1978.tb03923.x
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    The hepatic transport of sodium [14C]taurocholate in foetal sheep (1979)
    Oxford, UK : Blackwell Publishing Ltd
    Clinical and experimental pharmacology and physiology 6 (1979), S. 0 
    Details
    ISSN: 1440-1681
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: 1. The biliary excretion of a tracer pulse of sodium [14C] taurocholate injected into the portal vein was compared in adult and near-term foetal sheep.2. Biliary excretion was virtually complete in both adult and foetus, although appreciably faster in the adult.3. These results indicate that at birth the mechanisms for bile salt uptake and excretion in the sheep liver are well established.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1440-1681.1979.tb00014.x
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Cloned fragments of the plasmid ColV,I-K94 specifying virulence and serum resistance (1979)
    [s.l.] : Nature Publishing Group
    Nature 279 (1979), S. 778-781 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] A cloned BamH1-generated fragment of ColV,I-K94 increased the virulence of Escherichia coli, causing an approximately 100-fold reduction in LD50 for chicks. A genetic determinant f or resistance to the bactericidal effects of serum was mapped to a 5,300 base-pair sequence within the ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/279778a0
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    Paper (German National Licenses)
    Fulltext
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