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UPTAKE OF MANGANESE BY CHROMAFFIN GRANULES IN VITRO (1979)

Daniels, A. J. ; Johnson, L. N. ; Williams, R. J. P.

Oxford, UK : Blackwell Publishing Ltd

Journal of neurochemistry 33 (1979), S. 0

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ISSN: 1471-4159

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Medicine

Notes: Abstract– The distribution of metal ions in the chromaffin granule is anomalous. Calcium ions are accumulated but magnesium ions excluded. We have used the manganese ion as a convenient divalent element for following metal ion uptake because it can usefully be employed as a substitute for magnesium.The kinetics of the manganese incorporation were followed by radio tracer technique and its transport was characterized as being non-energy dependent and having a small thermal activation factor. The presence in the incubation medium of a membrane-bound ATPase inhibitor (NEM) or addition of CN− or NaN3 did not affect the metal incorporation into chromaffin granules. Little endogenous magnesium exchanges for manganese when the latter is incorporated, but magnesium competitively prevented manganese uptake. The binding of manganese inside the vesicle was strong and it was not exchangeable with magnesium even in the presence of ATP.Manganese when incorporated binds to ATP and displaces catecholamines resulting in a decrease in the CA/ATP ratio.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1111/j.1471-4159.1979.tb09922.x

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Crystallographic studies on the activity of glycogen phosphorylase b (1978)

Weber, I. T. ; Johnson, L. N. ; Wilson, K. S. ; [et al.]

[s.l.] : Nature Publishing Group

Nature 274 (1978), S. 433-437

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ISSN: 1476-4687

Source: Nature Archives 1869 - 2009

Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics

Notes: [Auszug] High resolution studies on the crystal structure of glycogen phosphorylase b have identified the catalytic site to which the substrate glucose-1-phosphate binds strongly with some local conformational changes. The site is situated 8 Å (phosphate-to-phosphate distance) from pyridoxal ...