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  • 1
    Electronic Resource
    Electronic Resource
    Characterisation of human cell lines and differentiation from HeLa by enzyme typing (1976)
    [s.l.] : Nature Publishing Group
    Nature 264 (1976), S. 60-63 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Table 1 Enzyme loci useful in characterising human cultured cells Enzyme Population group* Refs N. European Black Locus Phenotype Frequency Chance of Frequency Chance of of common 2 people of common 2 people phenotypes being alike phenotypes ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/264060b0
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  • 2
    Electronic Resource
    Electronic Resource
    The selection of somatic cell hybrids between human lymphoma cell lines (1976)
    Springer
    Somatic cell and molecular genetics 2 (1976), S. 309-324 
    Details
    ISSN: 1572-9931
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Somatic cell hybrids have been selected between three pairs of established human lymphoid cell lines producing pure lines of proliferating hybrid cells: Raji/Namalwa, Raji/Daudi, and Raji/BJAB. The hybrid cell lines have been characterized with respect to isozyme pattern, volume, and karyotype.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01538836
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  • 3
    Electronic Resource
    Electronic Resource
    Human gene mapping using an X/autosome translocation (1976)
    Springer
    Somatic cell and molecular genetics 2 (1976), S. 125-140 
    Details
    ISSN: 1572-9931
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Human fibroblasts containing a translocation between the X chromosome and chromosome 15 were fused with the 6-thioguanine-resistant mouse cell line, IR. Resulting hybrids, selected in HAT medium, retained the X/15 chromosome. Hybrids which were counterselected in 6-thioguanine lost this chromosome. The X-linked markers glucose-6-phosphate dehydrogenase (G6PD), phosphoglycerate kinase (PGK), and hypoxanthine phosphoribosyl transferase (HPRT), and the non-X-linked markers pyruvate kinase (PKM2) mannose phosphate isomerase (MPI), N-acetyl hexosaminidase A (HEXA) and β2-microglubulin (β2-m) all segregated in concordance with the X/15 translocation chromosome. The latter markers have been assigned to chromosome 15. Selection against the X/15 chromosome was done using antihuman β2-m serum. Electrophoretic and immunochemical analyses of the N-acetyl hexosaminidases A and B in these hybrids were performed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01542626
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  • 4
    Electronic Resource
    Electronic Resource
    Human intraspecific somatic cell hybrids: A genetic and karyotypic analysis of crosses between lymphocytes and D98/AH-2 (1975)
    Springer
    Somatic cell and molecular genetics 1 (1975), S. 41-64 
    Details
    ISSN: 1572-9931
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract A number of human intraspecific hybrids were produced by fusing the 8-azaguanine-resistant cell line D98/AH -2 with PHA-stimulated lymphocytes from a normal human male, followed by selection in HAT medium. The parent cells differed in zymogram patterns for 4 enzyme systems. Hypoxanthine-guanine phophoribosyltransferase was missing in D98/AH -2 and was determined in the hybrids by the normal gene derived from the lymphocyte donor's X chromosome. The HL-A antigens of the lymphocyte donor as well as the W28 specificity from HeLa were easily recognized by a cytotoxicity assay on the hybrid cells, while D98/AH -2 itself was not killed in the normal way by any HL-A typing sera. The initial hybrid karyotype in all lines was relatively stable, but slow loss of chromosomes occurred following extended growth in culture. The importance of the culture conditions for the rate of chromosome loss was demonstrated. The behavior of several chromosomes was followed in the hybrids and their derivatives. There was relatively nonspecific loss of small numbers of chromosomes, showing that loss of chromosomes from both the D98/AH -2 and the normal lymphocyte parent can occur. Cell lines resistant to 6-thioguanine were selected from the sensitive hybrids. Most had lost the lymphocyte donor's X chromosome, thereby losing the only active allele for HGPRT present in the initial hybrids. However, one line, DMR41, apparently retained the X chromosome and may have a mutated allele for HGPRT. Two lines that are the products of spontaneous segregation are also described: DM4CS and DM17A.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01538731
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  • 5
    Electronic Resource
    Electronic Resource
    Detection after electrophoresis of enzymes involved in ammonia metabolism using L-glutamate dehydrogenase as a linking enzyme (1977)
    Springer
    Biochemical genetics 15 (1977), S. 1023-1035 
    Details
    ISSN: 1573-4927
    Keywords: ammonia metabolism, enzymes of ; L-glutamate dehydrogenase as a linking enzyme
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The use of L-glutamate dehydrogenase (GLUD) as a reagent in staining mixtures to detect the isozymes of enzymes which catalyze the production of ammonia has been investigated. Methods have been devised for the electrophoresis and detection, using GLUD, of seven enzymes: cytidine deaminase, adenosine deaminase, adenosine monophosphate deaminase, arginase, argininosuccinase, D-amino acid oxidase, and D-aspartate oxidase. GLUD-linked staining methods appear to be sensitive, specific, and of general application.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00484494
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