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Digitale Medien

Detection of IgG Aggregates or Immune Complexes Using Solid-Phase C1q and Protein A-Rich Staphylococcus aureus as an Indicator System (1975)

FARRELL, C ; SØGAARD, H ; SVEHAG, S.-E.

Oxford, UK : Blackwell Publishing Ltd

Scandinavian journal of immunology 4 (1975), S. 0

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Details

ISSN: 1365-3083

Quelle: Blackwell Publishing Journal Backfiles 1879-2005

Thema: Medizin

Notizen: A radioimmunoassay for detection of Clq-binding IgG aggregates and antigen-IgG antibody complexes is described The assay makes use of solid-phase Clq and 32p-labelled protein A-rich Stphylococcusaureus as an indicator system. Both 19S and heavier IgG aggregates that fixed Clq were detected The sensitivity of the assay permitted detection of heavy (19–25S) IgG aggregates at a concentration of 8 μg/ml or less. The results indicated that detection of IgG in this assay is dependent on the degree of IgG polymerization and the molar ratio between the solid-phase Clq and the IgG polymers. Albumin-anti-albumin complexes, preformed at equilibrium with antibody to antigen molar ratios of 2:1 to 3:1 and at antigen concentrations of 25 to 40 μg/ml. were also detectable using the described radioimmunoassay

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1111/j.1365-3083.1975.tb02675.x

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