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  • 1
    Electronic Resource
    Electronic Resource
    Freeze-Preservation of Rice Cells Grown in Suspension Culture (1979)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 45 (1979), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: A simple procedure has been worked out for the freeze-preservation of rice (Oryza sativa L.) cells grown in suspension culture. The protocol differs in some interesting aspects from those established for other organisms. A peculiar feature of this procedure is that growth of freeze-recovered rice cells resumes after an extremely short lag period of 2–8 days and proceeds with a growth rate identical to that of untreated cells. This, together with data obtained with viability tests, rules out the possibility that selection of freeze-resistant mutant cells may occur, as postulated with other plant cells where growth resumption was considerably delayed in time.The viability of freeze-recovered rice cells, when assessed at time zero after thawing by measuring the mitochondrial respiratory efficiency, was 60–65% of that of untreated cells. However, the limits of this and other viability tests in determining the efficiency of the freeze preservation methods and the percentage of surviving cells were shown by experiments in which cell viability and cell growth were followed in cultures initiated with freeze-recovered rice cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1979.tb01682.x
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  • 2
    Electronic Resource
    Electronic Resource
    Effect of fusicoccin on plant cell cultures and protoplasts (1977)
    Springer
    Planta 135 (1977), S. 199-201 
    Details
    ISSN: 1432-2048
    Keywords: Cell suspension culture ; Fusicoccin ; 3-O-Methylglucose uptake ; Potassium ion uptake ; Proton extrusion ; Protoplasts
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have assayed the capacity of the fungal toxin fusicoccin to induce some of its characteristic effects (acidification of the medium, stimulation of K+, and of 3-O-methyl-D-glucose uptake) in cell suspensions of Parthenocissus tricuspidata (Siebold et Zucc.) Planchon, Acer pseudoplatanus L. and Oryza sativa L., and in protoplast suspensions prepared from leaves of Nicotiana tabacum L. and Spinacia oleracea L. or from cultures of P. tricuspidata. Evidence is presented showing that all tested biological materials respond to the addition of fusicoccin. The observation that the toxin is also active on protoplasts indicates that the cell wall is not involved in the mechanism of action of fusicoccin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00387171
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    The major DNA polymerase in cultured plant cells: Partial purification and correlation with cell multiplication (1979)
    Springer
    Planta 146 (1979), S. 521-527 
    Details
    ISSN: 1432-2048
    Keywords: DNA polymerase ; Plant cell suspension culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A DNA polymerase activity was isolated from cells of Oryza sativa L. grown in suspension culture. Molecular mass (∼ 180,000), optimal requirements for pH (neutral), Mg2+ (5–10 mM), Mn2+ (1 mM), template preference (activated DNA), lack of activity with native or denatured DNA, and sensitivity to N-ethylmaleimide and ionic strength are similar to those of the vertebrate α-polymerase. Like DNA polymerase α, the DNA polymerase described in this work is the most abundant in proliferating cells of Oryza sativa L., Parthenocissus tricuspidata (Siebold et Zucc.) Planchon, Acer pseudoplatanus L., and Medicago sativa L. and responds to changes in the rate of cell multiplication. We therefore postulate that this α-like DNA polymerase is the replicating enzyme of plant cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00388827
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    Paper (German National Licenses)
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