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  • 1
    Electronic Resource
    Electronic Resource
    Regeneration of Mesophyll Protoplasts Isolated from Dihaploid Clones of Solanum tuberosum (1978)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 43 (1978), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Protoplasts have been isolated from leaves of shoot cultures of six dihaploid clones of Solanum tuberosum L. (2n = 2x = 24). In the KM medium (Kao and Michayluk 1975), sustained cell divisions were obtained in up to 50% of the plated protoplasts of four clones, whereas only a few divisions occurred in the other two clones. The first mitosis appeared 2–8 days after plating, dependent on the clones. In the clones showing sustained cell divisions, a protoplast titre of about 5 × 103 per ml turned out to be optimal. The culture conditions for protoplasts of one of the poorly growing clones, clone H2 140, have been improved using modified KM media, plating at a concentration of as high as 5 × 104 cells per ml, and subsequent diluting at intervals 5 days. The dilutions were carried out with media containing 0.25% agar. Up to 60% of the plated protoplasts underwent divisions within 10 days under these conditions. After about 15 days, the regenerants were transferred onto media inducing organogenesis. Shoots and roots were formed on modified media MS (Murashige and Skoog 1962) and B5 (Gamborg et al. 1968). Plants have been regenerated in four of the investigated clones. Countings of chromosomes revealed a satisfactory stability of the karyotype in shoot culture and protoplast regeneration.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1978.tb01565.x
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  • 2
    Electronic Resource
    Electronic Resource
    Selection of somatic hybrids after fusion of protoplasts from Datura innoxia Mill. and Atropa belladonna L. (1979)
    Springer
    Planta 145 (1979), S. 371-375 
    Details
    ISSN: 1432-2048
    Keywords: Atropa ; Datura ; Morphogenesis ; Somatic hybridization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract After fusion of protoplasts from a diploid (2n=24) and a tetraploid (4n=48) chlorophyll-deficient mutant of Datura innoxia Mill. with diploid (2n=72) green wild-type protoplasts of Atropa belladonna L. thirteen somatic hybrids could be selected, most of which had already started to produce leaves and shoots. Hybrid calli were recognizable by the production of hairs, typical for Datura innoxia, and the green colour, derived from Atropa belladonna. Further proof for the hybrid nature was furnished by cytological investigations. The metaphase chromosomes of both species are easily distinguishable in their size: chromosomes of Datura innoxia are about twice as large as those of Atropa belladonna. The chromosome numbers of the hybrids varied from ca. 84 to ca. 175.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00388363
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  • 3
    Electronic Resource
    Electronic Resource
    Hybridisation experiments with protoplasts from chlorophyll-deficient mutants of some Solanaceous species (1977)
    Springer
    Planta 137 (1977), S. 253-257 
    Details
    ISSN: 1432-2048
    Keywords: Fusion ; Protoplast ; Solanaceae ; Somatic hybrid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Following fusion between protoplasts from two different chlorophyll-deficient diploid mutants of Datura innoxia Mill. it was possible to select 33 green hybrid calli on agar culture medium. Half of the somatic hybrids gave rise to leaves and some to shoots. The chromosome number of 20 somatic hybrids was determined: five were tetraploid, eight hexaploid, three octoploid, and four showed an aneuploid chromosome number. After transfer of the shoots of the five tetraploid hybrids to soil they developed roots. In control experiments in which protoplasts of the two mutants were cultured either as a mixture without being treated with the fusion agent, or cultured separately, no green callus could be obtained. Similar experiments involving protoplasts from one chlorophyll-deficient mutant of Datura innoxia, on the one hand, and those from similar mutants of Nicotiana sylvestris Spegazz. et Comes and Petunia hybrida, on the other, yielded no green somatic hybrid although hybrid protoplasts could be detected.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00388159
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  • 4
    Electronic Resource
    Electronic Resource
    Comparison of single cell culture derived Solanum tuberosum L. plants and a model for their application in breeding programs (1979)
    Springer
    Theoretical and applied genetics 55 (1979), S. 49-55 
    Details
    ISSN: 1432-2242
    Keywords: Microspore culture ; Protoplast culture ; Fusion of protoplasts ; Potato breeding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The techniques of microspore and protoplast regeneration starting from dihaploid Solanum tuberosum plants has been improved to such an extent that the production of more than 2000 microspore derived A1 plant lines and of several hundred protoplast derived plantlets has become possible. Further, from the dihaploid Solanum species S. phureja the regeneration of microspores to plants, and from the species S. infundibuliforme, S. sparsipilum and S. tarijense the regeneration of protoplasts to calluses, has been achieved. The plants descending from the two single cell culture systems are compared with reference to phenotypic markers and economic qualities. Some principles characteristic for either microspore or protoplast derived plants are examined and their significance is discussed. The results are compiled into an extended analytical synthetic breeding scheme based on a stepwise reduction of the autotetraploid to the monohaploid level and a subsequent controlled combination to a new synthetic completely heterozygous tetraploid potato.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00285189
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