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  • 1
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Journal of the American Chemical Society 100 (1978), S. 2389-2399 
    ISSN: 1520-5126
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Pediatric radiology 8 (1979), S. 165-167 
    ISSN: 1432-1998
    Keywords: Gamma camera renography ; Vesico-ureteral reflux ; Intravenous radionuclide cystography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract This study shows that Radionuclide imaging provides a simple method for detecting vesico-renal reflux in children, when an intravenous dose of 99mTc-D. T. P. A. is used as radioactive tracer. Forty-eight patients have been studied, of these twenty-eight also had intravenous pyelography with post-micturition films, micturating cystography, and cystoscopy. In this group of 28 patients the radionuclide imaging technique detected 25 refluxing ureters and 9 cases of bilateral vesico-renal reflux. Micturating cystography detected 12 refluxing ureters and only 3 cases were bilateral. Cystoscopy revealed 20 abnormal ureteric orifices and 6 patients had bilaterally abnormal orifices. Radionuclide imaging agreed with both the micturating cystograms and cystoscopic findings that reflux was occuring in 6 ureters. Radionuclide imaging agreed with the impression at cystoscopy that reflux was present in 18 ureters. Only 6 of the ureters diagnosed by micturating cystography as having reflux had abnormal ureteric openings at cystoscopy. The intravenous radionuclide imaging technique avoids the unpleasantness of catheterisation and its attendant risk of introducing infection. A lower dose of radiation is received than during radiological techniques. A renogram is obtained as part of the test. We believe this intravenous radionuclide imaging technique is a more “physiologically correct” test for vesico-renal reflux than any of the methods using catheterisation. Its limitations are that it is not so easy to use in hyperactive toddlers, nor does it give the anatomical definition that radiological techniques provide although improvements are expected with the latest gamma cameras and their associated equipment.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-0878
    Keywords: Median eminence ; Greenfinch (Chloris chloris L.) ; Anti-synthetic LHRH serum ; Binding sites ; Immunofluorescent localization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary With an indirect immunofluorescence technique and an antiserum raised against synthetic LHRH, immunofluorescent granules were observed in fine radially oriented fibres in the palisade layer of the zona externa in both the anterior and the posterior divisions of the median eminence in the greenfinch. There was no specific immunofluorescence in the tractus supraoptico-hypophyseus. It is concluded that the greenfinch median eminence contains material which is similar to mammalian LHRH.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1432-0878
    Keywords: Adenohypophysis, duck ; Gonadotrophes ; Immunofluorescence ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary An indirect immunofluorescence technique and an anti-chicken LH serum were used to localize cells in the adenohypophyses of drakes at different stages of their breeding cycle, after castration, and after castration combined with thyroxine treatment. Immunofluorescent cells were distributed throughout both lobes of the adenohypophyses from control and experimental birds and were shown to be alcian blue positive, PAS negative, basophiles. Immunofluorescent cells were as numerous in castrated birds as in castrated birds treated with thyroxine. Adjacent thin and semi-thin sections were used to study the cells binding anti-LH serum at light microscope and ultrastructural levels. The cells contained spherical granules with variable densities and diameters ranging between 40 and 280 nm in the rostral (= cephalic) lobe, and between 60 and 260 nm in the caudal lobe. The light microscope and ultrastructural observations showed that the anti-LH serum binds to cells which have been classified by other authors in the Pekin duck, quail and pigeon as TSH producing delta cells. The experimental technique used did not permit a distinction to be made between cells producing FSH and LH.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1432-0878
    Keywords: Immunocytochemistry ; Thyroid stimulating hormone (TSH) ; Pituitary gland ; Duck
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Cells binding anti-bovine TSH β serum were found exclusively in the rostral lobe of the adenohypophysis of the drake using the peroxidase-antiperoxidase complex unlabelled antibody method. The specificity of the binding of the anti-serum to TSH cells was established by relating the morphology and relative abundance of immunochemically stained cells to the TSH content of the adenohypophysis after experimentally altering the activity of the pituitary-thyroid axis. The TSH activity of the adenohypophysis was assessed indirectly, by the weight of the thyroid glands, and directly, by bioassay. As determined by bioassay, the TSH content of the rostral lobe of the adenohypohysis was much greater than that of the caudal lobe. Compared with control drakes, immunochemically stained cells in birds fed a goitrogen, methimazole, seemed to be enlarged and were closer together, while the stained cells in drakes injected with thyroxine were shrunken and less intensely stained. The TSH content of the adenohypophysis was increased in drakes fed methimazole. Castration did not alter the TSH content of the adenohypophysis or change the morphology of immunochemically stained cells. These observations suggest that in the drake: 1) anti-bovine TSH β serum binds specifically to TSH cells; 2) the TSH cells occur in the rostral and not in the caudal lobe of the adenohypophysis; and 3) the activity of TSH cells is not inhibited by the feedback effects of gonadal steroids.
    Type of Medium: Electronic Resource
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