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  • 1
    Electronic Resource
    Electronic Resource
    Production, purification and properties of a Pichia kluyveri killer toxin (1979)
    Springer
    Antonie van Leeuwenhoek 45 (1979), S. 437-450 
    Details
    ISSN: 1572-9699
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Production of the killer toxin of Pichia kluyveri 1002 was stimulated in the presence of yeast extract. In a minimal medium production was optimal at pH 3.8–4.0 and 22–25°C. Addition of gelatin and nonionic detergents, like Brij-58 (polyoxyethylene 20 cetyl ether) and Triton-X-100, to this medium enhanced production significantly. The killer toxin was purified 140-fold by use of a stepwise ethanol precipitation and butyl Sepharose column chromatography. The purified killer toxin, which still contained some carbohydrates, appeared to be glycoprotein with a mol wt of about 19000 and an isoelectric point of 4.3. It was stable between pH 2.5 and 4.7 and up to 40°C.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00443282
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    The effect of amino acids on the motile behavior of Bacillus subtilis (1977)
    Springer
    Archives of microbiology 113 (1977), S. 153-158 
    Details
    ISSN: 1432-072X
    Keywords: Chemotaxis ; Motility ; Velocity ; Methionine starvation ; Bacillus subtilis 60015
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Constant levels of amino acids enhanced the velocity of Bacillus subtilis 60015 cells about 2-fold and stimulated the response in motility assays. The stimulation of velocity did not occur via the receptors for chemotaxis. Cysteine and methionine, general inhibitors of chemotaxis, both completely inhibited the smooth response in a temporal gradient of attractant. After methionine starvation B. subtilis 60015 showed no measurable response in a temporal gradient of attractant, this in contrast to the effect observed with some other bacteria. Addition of methionine to starved cells restored the response toward attractant. Revertants of B. subtilis 60015 for methionine requirement could not be starved and showed a normal behavior toward temporal gradients of attractant.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00428596
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Polyribosomes in different stages of the life cycle of the water mold Allomyces arbuscula (1975)
    Springer
    Archives of microbiology 102 (1975), S. 117-122 
    Details
    ISSN: 1432-072X
    Keywords: Actinomycin ; Allomyces ; Gametogenesis ; Nuclear Cap ; Polyribosomes ; RNA Synthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Synchronous gametogenesis in the water mold Allomyces arbuscula is blocked by actinomycin D added at the onset of the process. Formation of the male gametangium can be selectively inhibited by administering actinomycin one hr after the induction of gametogenesis. The polyribosome pattern obtained after density gradient centrifugation remains virtually unchanged throughout gametogenesis until a stage immediately preceding maturation of the gametes. When ribosomes from gametes and swarming zygotes are analyzed on gradients, some RNase-sensitive material is found to band in the heavier portion of the gradient. Its presence suggests that some messenger RNA associated with ribosomes is conserved in the swarming cells. During gametogenesis RNA is de novo synthesized and becomes associated with the polyribosomes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00428355
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Induction of meiosis in Saccharomyces cerevisiae at different points in the mitotic cycle (1976)
    Springer
    Planta 130 (1976), S. 131-136 
    Details
    ISSN: 1432-2048
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Saccharomyces cells induced to undergo meiosis when in late G 1 or early S-phase, proceed mitotically until a point between completion of the S-phase and nuclear division. From that point, the cells start meiotic development without intervention of a round of premeiotic DNA replication. Cells induced at any other point in the cell cycle, enter meiosis from G 1.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00384409
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Chemotaxis and transport of amino acids in Allomyces arbuscula (1976)
    Springer
    Antonie van Leeuwenhoek 42 (1976), S. 203-209 
    Details
    ISSN: 1572-9699
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Among a number of amino acids tested, l-lysine and l-arginine are the principal attractants in the chemotaxis of the zygotes of Allomyces arbuscula. The reaction can be stimulated to a greater or lesser extent by a number of compounds chemically related to l-leucine. No relationship between transport of attracting amino acids and their effect on chemotaxis has been found.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00394116
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  • 6
    Electronic Resource
    Electronic Resource
    Killer-sensitive relationships in yeasts from natural habitats (1977)
    Springer
    Antonie van Leeuwenhoek 43 (1977), S. 125-128 
    Details
    ISSN: 1572-9699
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Yeast strains (157) belonging to at least 9 genera were isolated from natural habitats and screened for killer-sensitive relationships. Killer and sensitive characteristics were exhibited by 17 and 11 percent of the isolates, respectively. The strains belong to either one of two mutually exclusive killer-sensitive groups.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00395667
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    Paper (German National Licenses)
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