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  • 1975-1979  (3)
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Material
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Year
  • 1
    Electronic Resource
    Electronic Resource
    Simple differential Giemsa staining of sister chromatids after treatment with photosensitive dyes and exposure to light and the mechanism of staining (1975)
    Springer
    Chromosoma 53 (1975), S. 223-230 
    Details
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The essential steps of the 33258 Hoechst-Giemsa method for differential chromatid staining consist of (1) 33258 Hoechst treatment, (2) exposure to light, and (3) Giemsa staining. The staining was shown to be a function of the concentration of 33258 Hoechst and the light exposure. The dye was successfully replaced by various metachromatic dyes such as thionine. Two simple methods are proposed. Failure of the pale stained chromatids to restore Giemsa affinity with urea and trypsin and the diminished Feulgen reaction after light exposure suggest that not masking proteins but photolysis of the BrdU-incorporated chromatid components in the presence of photosensitive dyes play a role in the differential staining.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00329173
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Factors involved in differential giemsa-staining of sister chromatids (1978)
    Springer
    Chromosoma 66 (1978), S. 351-359 
    Details
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Microspectrophotometric evaluation of differentially stained sister chromatids made it possible to analyse precisely the factors involved in the Giemsa methods. The concentration of Hoechst 33258, pH of the mounting medium, temperature during UV-exposure and the quality (wavelength) of UV-light influenced the differential staining. Exposure of blacklight of 10−5 M Hoechst 33528-stained BrdU-labeled chromosome specimens mounted in McIlvaine buffer (pH 8.0) at 50° C reproducibly allowed differential staining of sister chromatids within 15 min. On the other hand, Korenberg-Freedlender's method using no Hoechst 33258 was also UV-light-dependent. Thus, photolysis of BrdU-substituted DNA was considered the basic mechanism of the Giemsa methods where the photosensitive Hoechst 33258 played a role as a sensitizer.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00328535
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    2.4-µm mapping of the galactic central region (1977)
    [s.l.] : Nature Publishing Group
    Nature 265 (1977), S. 515-516 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Our first observation by a balloon-borne telescope with wide spectral response (1.5-2.5 µm) was subject to unexpected temporal and spatial irregularities of the OH airglow emission1. For near infrared observation of the diffuse light at balloon altitudes we thus limited the wavelength range ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/265515a0
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    Paper (German National Licenses)
    Fulltext
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