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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Planta 146 (1979), S. 463-466 
    ISSN: 1432-2048
    Keywords: Legumin ; Pisum ; Protein (seeds) ; Storage proteins ; Sulphur ; Vicilin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract By means of crossed immunoelectrophoresis of the cotyledonary storage proteins of Pisum sativum L. it was shown that reduced accumulation of the legumin fraction, resulting from severe sulphur deficiency during growth, is accompanied by relative suppression of a quantitatively minor storage protein (Peak 3) shown previously by subunit analysis to be related to the vicilin series of holoproteins. The pattern of isotopic labelling of the storage proteins after injection of [35S]methionine into the pedicel during seed development under normal nutritional conditions indicated that Peak-3 protein, like legumin, has a relatively high content of sulphur amino-acids. Like certain of the vicilin molecules carrying the determinants responsible for Peak-4, Peak-3 protein binds selectively to concanavalin A.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-4927
    Keywords: Calliphora ; calliphorin ; storage protein ; hemolymph ; fat body ; mRNA ; in vitro translation ; in situ hybridization ; structural gene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract A major poly(A)-containing RNA fraction of the approximate size expected of a monocistronic mRNA for the storage protein calliphorin has been isolated from the larval fat bodies of Calliphora vicina during early instar 3. This 20 S RNA fraction programs the synthesis by cell-free wheat embryo extracts of polypeptides of 86,000 daltons identified by tryptic peptide fingerprinting as precursors of the authentic calliphorin subunits of 83,000 daltons. Complementary DNA synthesized by AMV reverse transcriptase using the same 20 S RNA as template hybridized in situ to a single segment of one or two bands in the salivary polytene chromosomes of C. vicina.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    European journal of applied physiology 36 (1977), S. 207-213 
    ISSN: 1439-6327
    Keywords: Anaerobic training ; Human skeletal muscle ; Phosphagens ; LDH ; Anaerobic capacity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The effects of a 6 week program of intense, intermittent hill running was investigated in 5 endurance-trained men (34–37 years). Venous lactate responses averaged 10.7 mM×1−1 during training. Biopsies were obtained from the vastus lateralis before and after training for determinations of histochemical classification, phosphagen concentrations (ATP and CP) and activities of total LDH and selected LDH isozymes. Indices of aerobic and anaerobic capacity were also measured. Significant improvements (P〈0.01) were noted in training distance and loads employed in leg presses, also part of the training: the treadmill test for anaerobic capacity improved by 16.7% and the terminal blood lactate level increased 14% (P〈0.05). No changes occurred in $$\dot V$$ O2 max, body fat per cent or anaerobic power. Muscle ATP concentration increased by 14.8% (P〈0.01) whereas the tissue level of CP remained unchanged. Total LDH activity and the LDH isozymes (LDH-1, LDH-5 and LDH-2, 3, 4) were not altered by training. No changes in fibre distribution were observed. That adaptations to the anaerobic training did occur is evident, but the cellular locus remains to be elucidated further.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1439-6327
    Keywords: Athletes ; High intensity training ; Muscle fiber types ; Fiber size and metabolic potential
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In order to determine the influence of an extensive history of participation in high intensity activity on muscle fiber type, fiber size, and metabolic profile, elite ice hockey players were selected for investigation from three different leagues. Biopsy samples from the vastus lateralis muscle were obtained from different groups of players prior to and following the season and compared with control subjects. No significant differences were found in the percentage (49.6 vs. 43.8%) or the size of the ST fibers between the elite athletes and the control group, nor was there any significant alteration following the season of play in these variables. For the FT fiber subgroups, a reduction in the FTb (12.2 vs. 3.9%) and an increase in FTa (38.0 to 45.2%) fiber populations occurred over the season. Similarly, increases in fiber area were observed for both FT subgroups pre to post season. Of the enzymes studied only 3-hydroxyacyl CoA dehydrogenase was elevated in the post season measures, while total phosphorylase and phosphofructokinase were significantly lower. The metabolic pattern exhibited does not appear to be substantially different from what would be expected from an untrained group of similar fiber distribution.
    Type of Medium: Electronic Resource
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