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Acridine orange staining of the mammalian fibroblast cell coat (1979)

Timár, J. ; Gyapay, G. ; Lapis, K.

Springer

Histochemistry and cell biology 64 (1979), S. 189-193

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ISSN: 1432-119X

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Medicine

Notes: Summary Acridine orange selectively binds to glycos- and galactosaminoglycan (GAG) compunds in the presence of Na+ in low concentrations. We have worked out a cytochemical method, which is suitable for the specific demonstration of surface GAG components. The method consists of a glutaraldehyde prefixation, an acridine orange block-staining for 48 h and an OsO4 postfixation for some hours. The specificity of the staining was verified with the help of fluorimetric-in vitro-measurements and enzymatic digestions, respectively.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00490098

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