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  • 1
    Electronic Resource
    Electronic Resource
    THE FATE OF AFLATOXINS IN THE PREPARATION OF PROTEIN CONCENTRATES AND ISOLATES FROM CONTAMINATED PEANUT AND COTTONSEED FLOURS (1976)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 41 (1976), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: With the assistance of the laboratories of six major soybean protein producers, simulated commercial protein concentrate and isolate processes were applied to aflatoxin-contaminated peanut and cottonseed flours to determine the fate of the aflatoxins. Processes for producing, concentrate by washing the flour at the protein isoelectric point (pH 4.5) were ineffective in separating the aflatoxin from the products, but a process using an aqueous alcohol wash accomplished a 90% reduction in the aflatoxin level of the concentrate compared to the flour. Processes for producing isolate by protein dissolution in alkali and its recovery by precipitation at the isoelectric point resulted in some aflatoxin loss but an increase in the aflatoxin concentration associated with the protein isolate compared to the original flour. However, a process applying carbon adsorbent to the alkaline solution accomplished a 90% reduction in the aflatoxin level of the isolate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.1976.tb14435.x
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  • 2
    Electronic Resource
    Electronic Resource
    The concentration of Cytochrome f and P700 in chlorophyll-deficient mutants of Chlorella fusca (1977)
    Springer
    Planta 136 (1977), S. 281-282 
    Details
    ISSN: 1432-2048
    Keywords: Chlorophyll-deficient mutants ; Cytochrome f ; P700 ; Photosynthetic unit
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The ratio of Chlorophyll: Cytochrome f and of Chlorophyll: P700 (reaction center pigment in photosystem I) is essentially lower in chlorophyll-deficient mutants than in the normal green strain. On a dry weight basis, the mutants have the same or a higher content of redox enzymes than the normal form. The size of the photosynthetic unit of the mutants is 4 to 7 times smaller than that of the normal strains, due mainly to a deficiency of the light-harvesting chlorophyll-protein complex.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00385997
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  • 3
    Electronic Resource
    Electronic Resource
    Development of the photosynthetic apparatus during light-dependent greening of a mutant of Chlorella fusca (1978)
    Springer
    Planta 140 (1978), S. 45-52 
    Details
    ISSN: 1432-2048
    Keywords: Cytochrome f ; Development photosynthetic apparatus ; Greening mutant ; Photochemical activities ; Photosynthetic unit ; P-700 ; Ribulose bisphosphate carboxylase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The formation of chlorophyll, cytochrome f, P-700, ribulose bisphosphate carboxylase as well as photosynthesis and Hill reaction activities were tested during the light-dependent greening process of the Chlorella fusca mutant G 10. Neither chlorophyll nor protochlorophyllide was detected in the darkgrown cells. When transferred to light the mutant cells developed chlorophyll and established its photosynthetic capacity after a short lag phase. In the in vivo absorption spectra a spectral shift of the red absorption peak position from 674 to 680 nm was indicated during the first 3 h of greening. Cytochrome f was already present in the dark-grown cells, but during the greening phase a threefold increase in the cytochrome f content could be seen. At the early stages of greening a characteristic primary oscillation in the content of cytochrome f was observed. P-700 was lacking in the dark and during the first 30 min of illumination. From the first to the second h of light a forced synthesis of P-700 took place and the time-course curve for the ratios of P-700/chlorophyll rose to a sharp maximum. The synthesis of P-700 started together with photosystem I activity and showed similar kinetics. We found the simultaneous appearance of photosystem II, photosystem I, and photosynthetic activities 30 min after the beginning of the illumination. Based on chlorophyll content they attained maximum activity after 2 h of light, but at this time photosystem I capacity proved to be remarkably higher than photosynthetic and photosystem II activities. Highest carboxylase activity existed in darkgrown cells. During the greening process the activity of the enzyme decreased continuously. After 2 h of illumination chlorophyll synthesis partially served to increase the size of the photosynthetic unit, which consequently led to a decrease in the light energy needed to saturate photosynthesis and also to a decrease of photosynthetic rate based on chlorophyll content.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00389378
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  • 4
    Electronic Resource
    Electronic Resource
    The intensification of absorbance changes in leaves by light-dispersion (1979)
    Springer
    Planta 146 (1979), S. 551-557 
    Details
    ISSN: 1432-2048
    Keywords: Cytochrome f ; High-light plants ; Low-light plants ; Light-dispersion ; P-700
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In dispersive samples, like leaves, the absorbance of pigments is intensified. The intensification is due to a longer optical path through the dispersive sample. However, in chloroplast suspensions the optical path is not much longer than in clear solutions. The factor of intensification β (=the lengthening of the optical path) is calculated by comparing the absorbance of leaves and the absorbance of chloroplast suspensions with equal pigment-content. This method also includes the influence of possible sieve effects which could decrease absorbance. The measurements are carried out with high- and low-light leaves of different thickness and pigment content. The intensification of absorbance was 2–2.5 fold. In highlight leaves it was somewhat less than in low-light leaves. The factor β is better correlated to the pigment content than to the thickness of the leaves. The plot of absorbance versus the pigment content of the leaves shows that β decreases with increasing pigment content. In contrast, chloroplast suspensions show a linear dependence as expected from Lambert-Beer's law. Thus, in leaves with very low pigment content the absorbance is intensified up to 6 fold while the intensification decreases with increasing absorbance. These results are in good agreement with measurements of Tsel'niker (1975) and with the theoretical predictions of Butler's formula (1960). Absorbance changes due to photooxidation of P-700 and cytochrome f in intact leaves are measured, and β is used to calculate the amount of the oxidized components. Without correction for β the values would be much greater than the amount actually present. The corrected data show that between 70 and 90% of the present P-700 and cytochrome f can be photooxidized in the intact leaf.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00388831
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  • 5
    Electronic Resource
    Electronic Resource
    Measurements of cytochrome f and P-700 in intact leaves of Sinapis alba grown under high-light and low-light conditions (1979)
    Springer
    Planta 146 (1979), S. 377-385 
    Details
    ISSN: 1432-2048
    Keywords: Cytochrome f ; Electron-transport ; High-light plants ; Low-light plants ; P-700 ; Photosynthetic unit
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The oxidation and reduction of cytochrome f and P-700 is measured spectrophotometrically in leaves of low-light and high-light plants. After illumination with red light, an induction phenomenon for cytochrome f oxidation is observed which indicates a regulation of photosystem I activity through energy distribution between the pigment systems by the energy state of the membrane. After far-red excitation the reduction of cytochrome f in the dark is much slower in low-light leaves. This shows that cyclic electron transport is not improved in low-light plants under these conditions. P-700 is oxidized on excitation with far-red light. However, with high intensities of far-red light, P-700 is partially reduced again which is due to a low extent of photosystem II excitation with the far-red used in the experiments. The low-light leaves show greater sensitivity of photosystem II to this excitation. The initial rate of the cytochrome f oxidation-rate is the same in low-light and high-light leaves. This shows that several P-700 are connected with only one electron transport chain. The consequences of these results concerning the tripartite concept and the photosynthetic unit are discussed. In the high-light plants the experimental data can be well explained by the tripartite organization of the photosynthetic unit. In low-light plants, however, a multipartite organization has to be postulated. In the partition regions of the grana, several antennae systems I, antennae systems II, and light-harvesting complexes can communicate with one electron transport chain.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00380848
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  • 6
    Electronic Resource
    Electronic Resource
    Evidence for Fc receptors on rabbit yolk sac endoderm (1977)
    [s.l.] : Nature Publishing Group
    Nature 268 (1977), S. 443-445 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] To obtain yolk sac endodermal cells, conceptuses at 24-26 d of gestation were removed intact from pregnant Dutch or New Zealand White rabbits and washed free of contaminating blood with Hanks Balanced Salt Solution (BSS), pH7.5. This was used free of calcium and magnesium to aid cell separation in ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/268443a0
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  • 7
    Electronic Resource
    Electronic Resource
    Lens regeneration from cornea in tail ectopic eyes of Xenopus laevis tadpoles (1977)
    Springer
    Cellular and molecular life sciences 33 (1977), S. 1210-1211 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Evidence was found that cornea in tail ectopic eyes of Xenopus tadpoles has the capacity to regenerate a new lens.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01922334
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  • 8
    Electronic Resource
    Electronic Resource
    Direct evidence for pH-dependent Fc receptors on proximal enterocytes of suckling rat gut (1979)
    Springer
    Cellular and molecular life sciences 35 (1979), S. 838-840 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary By means of an erythrocyte-antibody rosette technique, Fc receptors, functional at pH 6.0 but not at 7.2, were shown to be present on enterocytes isolated from duodenum and jejunum (but absent from ileum) of 12–20-day-old suckling rats.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01968284
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  • 9
    Electronic Resource
    Electronic Resource
    Rate of nitrification of ammoniated vermiculite (1977)
    Springer
    Plant and soil 46 (1977), S. 633-636 
    Details
    ISSN: 1573-5036
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Treatment of a commercial sample of vermiculite (1–2 mm grade) in its natural (non-exfoliated) state with ammonia gas gave a product which nitrified more slowly than ammonium sulphate. The product has potential as a slow-acting fertilizer although its maximum nitrogen content is only 5–6 per cent. The product formed from exfoliated vermiculite and ammonia gas should be useful as a medium for plant growth because it combines good physical properties with a high rate of nitrogen release. re]19760730
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00015922
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  • 10
    Electronic Resource
    Electronic Resource
    Localisation of proteins in coated micropinocytotic vesicles during transport across rabbit yolk sac endoderm (1976)
    Springer
    Cell & tissue research 171 (1976), S. 175-193 
    Details
    ISSN: 1432-0878
    Keywords: Yolk sac ; Coated vesicles ; Proteins ; Transport ; Electronmicroscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Rabbit yolk sac splanchnopleur exposed in utero to IgG-HRP and IgG-ferritin conjugates, rabbit and bovine anti-HRP antibodies, free HRP, ferritin and human IgG, was examined ultrastructurally in an attempt to determine whether or not coated micropinocytotic vesicles are involved in selectively transporting immunoglobulins across yolk sac endodermal cells. Human, rabbit and bovine IgG-HRP conjugates, rabbit anti-HRP antibodies, free HRP and human IgG, all become localised in coated micropinocytotic vesicles. Differences were observed in that only human IgG and rabbit anti-HRP antibodies could be located in the intercellular space and bovine IgG-HRP conjugate could not be detected in coated micropinocytotic vesicles in confluence with the lateral and basal plasmalemma. Bovine anti-HRP antibodies, IgG-ferritin conjugates, and free ferritin, could not be observed in coated micropinocytotic vesicles. All proteins were detected in macropinocytotic vesicles, and dense bodies resembling phagolysosomes. Results are discussed in the light of a proposal that selection occurs at the cell surface during formation of coated micropinocytotic vesicles and is not linked to intracellular proteolysis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00219405
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