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  • 2005-2009  (1)
  • 1970-1974  (1)
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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 124 (2005), S. 0 
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Apple is a temperate fruit species with several breeding disadvantages such as the highly heterozygous nature of the genome and a long reproductive cycle. It is expected that homozygous apple genotypes can increase breeding efficiency, but inbreeding is prevented by the gametophytic self-incompatibility mechanism. In this paper, the production and characterization of homozygous genotypes, generated from anther culture in Malus × domestica cv. ‘Braeburn’, is described as well as their potential for use in breeding programmes. After determination of large scale anther culture efficiency, anther culture-derived genotypes were simple sequence repeat (SSR)-fingerprinted to determine their homozygous nature and confirm their androgenic origin. Their value for breeding was estimated based on growth and fertility measurements. The use of homozygous androgenic genotypes from ‘Braeburn’ in apple breeding programmes is currently not a realistic approach, partly because of the low efficiency of anther culture, but mainly because of the reduced vigour and severe sterility of the androgenic genotypes produced.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Archives of virology 33 (1971), S. 171-176 
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A micro-color test is described in which destruction of monolayers of secondary pig thyroid cells in disposable plastic trays as visualized macroscopically after staining with crystal violet is used as an indicator of viral multiplication. This test proved to be sensitive and to give reproducible results when applied to the titration of transmissible gastroenteritis (TGE) virus-neutralizing antibodies. It was, however, considerable less sensitive than a macro procedure when used for the titration of TGE virus.
    Type of Medium: Electronic Resource
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